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Article: Involvement of microRNA lethal-7a in the regulation of embryo implantation in mice

TitleInvolvement of microRNA lethal-7a in the regulation of embryo implantation in mice
Authors
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2012, v. 7 n. 5 How to Cite?
AbstractMicroRNAs interact with multiple mRNAs resulting in their degradation and/or translational repression. This report used the delayed implantation model to determine the role of miRNAs in blastocysts. Dormant blastocysts in delayed implanting mice were activated by estradiol. Differential expression of 45 out of 238 miRNAs examined was found between the dormant and the activated blastocysts. Five of the nine members of the microRNA lethal-7 (let-7) family were down-regulated after activation. Human blastocysts also had a low expression of let-7 family. Forced-expression of a family member, let-7a in mouse blastocysts decreased the number of implantation sites (let-7a: 1.1±0.4; control: 3.8±0.4) in vivo, and reduced the percentages of blastocyst that attached (let-7a: 42.0±8.3%; control: 79.0±5.1%) and spreaded (let-7a: 33.5±2.9%; control: 67.3±3.8%) on fibronectin in vitro. Integrin-β3, a known implantation-related molecule, was demonstrated to be a target of let-7a by 3′-untranslated region reporter assay in cervical cancer cells HeLa, and Western blotting in mouse blastocysts. The inhibitory effect of forced-expression of let-7a on blastocyst attachment and outgrowth was partially nullified in vitro and in vivo by forced-expression of integrin-β3. This study provides the first direct evidence that let-7a is involved in regulating the implantation process partly via modulation of the expression of integrin-β3. (200 words). © 2012 Liu et al.
Persistent Identifierhttp://hdl.handle.net/10722/173376
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiu, WMen_HK
dc.contributor.authorPang, RTKen_HK
dc.contributor.authorCheong, AWYen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorLao, Ken_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2012-10-30T06:29:46Z-
dc.date.available2012-10-30T06:29:46Z-
dc.date.issued2012en_HK
dc.identifier.citationPlos One, 2012, v. 7 n. 5en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/173376-
dc.description.abstractMicroRNAs interact with multiple mRNAs resulting in their degradation and/or translational repression. This report used the delayed implantation model to determine the role of miRNAs in blastocysts. Dormant blastocysts in delayed implanting mice were activated by estradiol. Differential expression of 45 out of 238 miRNAs examined was found between the dormant and the activated blastocysts. Five of the nine members of the microRNA lethal-7 (let-7) family were down-regulated after activation. Human blastocysts also had a low expression of let-7 family. Forced-expression of a family member, let-7a in mouse blastocysts decreased the number of implantation sites (let-7a: 1.1±0.4; control: 3.8±0.4) in vivo, and reduced the percentages of blastocyst that attached (let-7a: 42.0±8.3%; control: 79.0±5.1%) and spreaded (let-7a: 33.5±2.9%; control: 67.3±3.8%) on fibronectin in vitro. Integrin-β3, a known implantation-related molecule, was demonstrated to be a target of let-7a by 3′-untranslated region reporter assay in cervical cancer cells HeLa, and Western blotting in mouse blastocysts. The inhibitory effect of forced-expression of let-7a on blastocyst attachment and outgrowth was partially nullified in vitro and in vivo by forced-expression of integrin-β3. This study provides the first direct evidence that let-7a is involved in regulating the implantation process partly via modulation of the expression of integrin-β3. (200 words). © 2012 Liu et al.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshBlastocyst - metabolism-
dc.subject.meshDNA Primers - genetics-
dc.subject.meshEmbryo Implantation - genetics - physiology-
dc.subject.meshGene Expression Regulation, Developmental - genetics-
dc.subject.meshMicroRNAs - genetics - metabolism-
dc.titleInvolvement of microRNA lethal-7a in the regulation of embryo implantation in miceen_HK
dc.typeArticleen_HK
dc.identifier.emailPang, RTK: rtkpang@hku.hken_HK
dc.identifier.emailNg, EHY: nghye@hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.authorityPang, RTK=rp01761en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1371/journal.pone.0037039en_HK
dc.identifier.pmid22623977-
dc.identifier.pmcidPMC3356405-
dc.identifier.scopuseid_2-s2.0-84862099830en_HK
dc.identifier.hkuros209439-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84862099830&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue5en_HK
dc.identifier.isiWOS:000305343500054-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiu, WM=55161670200en_HK
dc.identifier.scopusauthoridPang, RTK=7004376636en_HK
dc.identifier.scopusauthoridCheong, AWY=24576433900en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridLao, K=35285715500en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridYeung, WSB=55763794910en_HK

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