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Article: Cumulus-associated α2-macroglobulin derivative retains proconceptive glycodelin-C in the human cumulus matrix

TitleCumulus-associated α2-macroglobulin derivative retains proconceptive glycodelin-C in the human cumulus matrix
Authors
KeywordsΑ2-Macroglobulin
Cumulus Matrix
Glycodelin
Hyaluronic Acid
Spermatozoa
Issue Date2009
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2009, v. 24 n. 11, p. 2856-2867 How to Cite?
AbstractBACKGROUNDGlycodelin-C is a glycodelin isoform isolated from the cumulus matrix. It stimulates spermatozoa-zona pellucida binding. Here, we report the isolation and characterization of a novel glycodelin interacting protein (GIP) from human cumulus matrix.METHODSGIP was purified by liquid chromatograph and identified by mass spectrometry. The interaction of GIP with glycodelin, matrix molecule and spermatozoa were investigated. RESULTS Mass spectrometry analysis suggested that GIP contained the N-terminal region of α2-macroglobulin, confirmed by western blot with anti-α2-macroglobulin antibody. GIP bound to native but not deglycosylated glycodelin-C in native gel electrophoresis, suggesting that the binding was glycosylation-dependent. GIP did not bind to capacitated and uncapacitated human spermatozoa. The cumulus cells could convert exogenous labeled α2-macroglobulin into GIP in vitro. GIP interacted with hyaluronic acid, a major component of the cumulus matrix. Glycodelin-C bound to hyaluronic acid-coated agarose beads in the presence of GIP. Human spermatozoa acquired the hyaluronic acid-GIP-bound glycodelin-C during incubation in vitro. CONCLUSION The hyaluronic acid-GIP complex formed in the cumulus matrix retains and concentrates glycodelin-C in the cumulus matrix for displacing sperm-bound glycodelin-A and-F and stimulating the zona binding activity of the spermatozoa traversing through the cumulus mass.
Persistent Identifierhttp://hdl.handle.net/10722/173353
ISSN
2014 Impact Factor: 4.569
2014 SCImago Journal Rankings: 1.972
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChung, MKen_HK
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorLee, CLen_HK
dc.contributor.authorPang, RTKen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorKoistinen, Ren_HK
dc.contributor.authorKoistinen, Hen_HK
dc.contributor.authorSeppala, Men_HK
dc.contributor.authorYeung, WSBen_HK
dc.date.accessioned2012-10-30T06:29:33Z-
dc.date.available2012-10-30T06:29:33Z-
dc.date.issued2009en_HK
dc.identifier.citationHuman Reproduction, 2009, v. 24 n. 11, p. 2856-2867en_HK
dc.identifier.issn0268-1161en_HK
dc.identifier.urihttp://hdl.handle.net/10722/173353-
dc.description.abstractBACKGROUNDGlycodelin-C is a glycodelin isoform isolated from the cumulus matrix. It stimulates spermatozoa-zona pellucida binding. Here, we report the isolation and characterization of a novel glycodelin interacting protein (GIP) from human cumulus matrix.METHODSGIP was purified by liquid chromatograph and identified by mass spectrometry. The interaction of GIP with glycodelin, matrix molecule and spermatozoa were investigated. RESULTS Mass spectrometry analysis suggested that GIP contained the N-terminal region of α2-macroglobulin, confirmed by western blot with anti-α2-macroglobulin antibody. GIP bound to native but not deglycosylated glycodelin-C in native gel electrophoresis, suggesting that the binding was glycosylation-dependent. GIP did not bind to capacitated and uncapacitated human spermatozoa. The cumulus cells could convert exogenous labeled α2-macroglobulin into GIP in vitro. GIP interacted with hyaluronic acid, a major component of the cumulus matrix. Glycodelin-C bound to hyaluronic acid-coated agarose beads in the presence of GIP. Human spermatozoa acquired the hyaluronic acid-GIP-bound glycodelin-C during incubation in vitro. CONCLUSION The hyaluronic acid-GIP complex formed in the cumulus matrix retains and concentrates glycodelin-C in the cumulus matrix for displacing sperm-bound glycodelin-A and-F and stimulating the zona binding activity of the spermatozoa traversing through the cumulus mass.en_HK
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_HK
dc.relation.ispartofHuman Reproductionen_HK
dc.subjectΑ2-Macroglobulinen_US
dc.subjectCumulus Matrixen_US
dc.subjectGlycodelinen_US
dc.subjectHyaluronic Aciden_US
dc.subjectSpermatozoaen_US
dc.subject.meshChromatography, Liquiden_HK
dc.subject.meshCumulus Cells - metabolismen_HK
dc.subject.meshExtracellular Matrix Proteins - metabolismen_HK
dc.subject.meshFemaleen_HK
dc.subject.meshGlycoproteins - metabolismen_HK
dc.subject.meshGlycosylationen_HK
dc.subject.meshHumansen_HK
dc.subject.meshHyaluronic Acid - metabolismen_HK
dc.subject.meshMaleen_HK
dc.subject.meshMass Spectrometryen_HK
dc.subject.meshPregnancy Proteins - metabolismen_HK
dc.subject.meshProtein Isoforms - metabolismen_HK
dc.subject.meshSpermatozoa - metabolismen_HK
dc.subject.meshalpha-Macroglobulins - metabolismen_HK
dc.titleCumulus-associated α2-macroglobulin derivative retains proconceptive glycodelin-C in the human cumulus matrixen_HK
dc.typeArticleen_HK
dc.identifier.emailChiu, PCN: pchiucn@hku.hken_HK
dc.identifier.emailPang, RTK: rtkpang@hku.hken_HK
dc.identifier.emailNg, EHY: nghye@hku.hken_HK
dc.identifier.emailLee, KF: ckflee@hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityPang, RTK=rp01761en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1093/humrep/dep265en_HK
dc.identifier.pmid19625311en_HK
dc.identifier.scopuseid_2-s2.0-70350510673en_HK
dc.identifier.hkuros171468-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-70350510673&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue11en_HK
dc.identifier.spage2856en_HK
dc.identifier.epage2867en_HK
dc.identifier.isiWOS:000271107700025-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridChung, MK=8964203600en_HK
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridLee, CL=9277221100en_HK
dc.identifier.scopusauthoridPang, RTK=7004376636en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridKoistinen, R=7006574669en_HK
dc.identifier.scopusauthoridKoistinen, H=7003612125en_HK
dc.identifier.scopusauthoridSeppala, M=35475165300en_HK
dc.identifier.scopusauthoridYeung, WSB=55763794871en_HK
dc.identifier.citeulike5426223-

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