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Article: Correlation of maternal plasma total cell-free DNA and fetal DNA levels with short term outcome of first-trimester vaginal bleeding

TitleCorrelation of maternal plasma total cell-free DNA and fetal DNA levels with short term outcome of first-trimester vaginal bleeding
Authors
Issue Date2007
PublisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/
Citation
Human Reproduction, 2007, v. 22 n. 6, p. 1736-1743 How to Cite?
AbstractBackground: The current methods using sonographic parameters and/or maternal serum β-HCG levels to predict spontaneous abortion are not satisfactory. The aim of this study was to determine whether maternal plasma fetal DNA and total DNA levels could be used to predict spontaneous abortion. Methods: We prospectively studied pregnant women who presented with vaginal bleeding in the first trimester of pregnancy, and those who had no vaginal bleeding (controls). DYS14 and the β-globin gene were used to measure the maternal plasma levels of fetal and total DNA, respectively, by real-time PCR. Results: A total of 1114 women were studied. Both maternal plasma fetal and total DNA concentrations increased with gestation from 6 to 11.6 weeks in the controls. The multiple of medians (MoMs) of fetal and total DNA concentration in those who miscarried were significantly greater (P < 0.001) than in the normal controls by about 5- and 4-fold respectively. Using a cut-off value of 1.6 MoMs for total DNA to predict spontaneous abortion, the sensitivity was 98.2% and false positive rate was 4.7%. However, using a cut-off value of 1.8 MoMs for fetal DNA, the corresponding figures were 97% and 44.3%, respectively. Conclusions: Both maternal plasma fetal and total DNA concentrations increased throughout the first trimester. Significantly high levels of fetal and total DNA were found in those who miscarried. © The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/173323
ISSN
2015 Impact Factor: 4.621
2015 SCImago Journal Rankings: 2.271
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYin, Aen_US
dc.contributor.authorNg, EHYen_US
dc.contributor.authorZhang, Xen_US
dc.contributor.authorHe, Yen_US
dc.contributor.authorWu, Jen_US
dc.contributor.authorLeung, KYen_US
dc.date.accessioned2012-10-30T06:29:20Z-
dc.date.available2012-10-30T06:29:20Z-
dc.date.issued2007en_US
dc.identifier.citationHuman Reproduction, 2007, v. 22 n. 6, p. 1736-1743en_US
dc.identifier.issn0268-1161en_US
dc.identifier.urihttp://hdl.handle.net/10722/173323-
dc.description.abstractBackground: The current methods using sonographic parameters and/or maternal serum β-HCG levels to predict spontaneous abortion are not satisfactory. The aim of this study was to determine whether maternal plasma fetal DNA and total DNA levels could be used to predict spontaneous abortion. Methods: We prospectively studied pregnant women who presented with vaginal bleeding in the first trimester of pregnancy, and those who had no vaginal bleeding (controls). DYS14 and the β-globin gene were used to measure the maternal plasma levels of fetal and total DNA, respectively, by real-time PCR. Results: A total of 1114 women were studied. Both maternal plasma fetal and total DNA concentrations increased with gestation from 6 to 11.6 weeks in the controls. The multiple of medians (MoMs) of fetal and total DNA concentration in those who miscarried were significantly greater (P < 0.001) than in the normal controls by about 5- and 4-fold respectively. Using a cut-off value of 1.6 MoMs for total DNA to predict spontaneous abortion, the sensitivity was 98.2% and false positive rate was 4.7%. However, using a cut-off value of 1.8 MoMs for fetal DNA, the corresponding figures were 97% and 44.3%, respectively. Conclusions: Both maternal plasma fetal and total DNA concentrations increased throughout the first trimester. Significantly high levels of fetal and total DNA were found in those who miscarried. © The Author 2007. Published by Oxford University Press on behalf of the European Society of Human Reproduction and Embryology. All rights reserved.en_US
dc.languageengen_US
dc.publisherOxford University Press. The Journal's web site is located at http://humrep.oxfordjournals.org/en_US
dc.relation.ispartofHuman Reproductionen_US
dc.subject.meshAbortion, Spontaneous - Diagnosisen_US
dc.subject.meshAdulten_US
dc.subject.meshDna - Blooden_US
dc.subject.meshFemaleen_US
dc.subject.meshGlobins - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshMaternal-Fetal Exchangeen_US
dc.subject.meshPregnancyen_US
dc.subject.meshPregnancy Trimester, First - Blooden_US
dc.subject.meshUterine Hemorrhage - Diagnosisen_US
dc.titleCorrelation of maternal plasma total cell-free DNA and fetal DNA levels with short term outcome of first-trimester vaginal bleedingen_US
dc.typeArticleen_US
dc.identifier.emailNg, EHY:nghye@hkucc.hku.hken_US
dc.identifier.authorityNg, EHY=rp00426en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1093/humrep/dem058en_US
dc.identifier.pmid17416916-
dc.identifier.scopuseid_2-s2.0-34447330701en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34447330701&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume22en_US
dc.identifier.issue6en_US
dc.identifier.spage1736en_US
dc.identifier.epage1743en_US
dc.identifier.isiWOS:000247470700036-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridYin, A=36908388100en_US
dc.identifier.scopusauthoridNg, EHY=35238184300en_US
dc.identifier.scopusauthoridZhang, X=16837492700en_US
dc.identifier.scopusauthoridHe, Y=7404942268en_US
dc.identifier.scopusauthoridWu, J=35081471700en_US
dc.identifier.scopusauthoridLeung, KY=8247106900en_US

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