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Article: A novel approach to protect fatty liver graft against I/R injury

TitleA novel approach to protect fatty liver graft against I/R injury
Authors
Issue Date2009
PublisherPabst Science Publishers. The Journal's web site is located at http://www.pabst-publishers.de/txmed
Citation
Transplantationsmedizin: Organ Der Deutschen Transplantationsgesellschaft, 2009, v. 21 SUPPL. 2, p. 17 How to Cite?
AbstractBackgrounds: Fatty liver accelerates the I/R injury after reperfusion that significantly affects the outcome of liver transplantation. In the study, we established a novel protein transduction system to prevent I/R injury of fatty livers. Methods and results: Modified human heme oxygenase-1 (HO-1) or enhanced green fluorescent protein (EGFP) recombinant protein containing a cell-penetrating domain (PTD) was generated. Their biological activities were investigated in a syngeneic liver transplantation model. PTD/HO-1 or PTD/EGFP was administered through portal vein after the graft harvesting and the grafts were preserved in cold UW solution. The targeted protein was found in the vessel wall of portal areas and adjuvant liver cells at 10 min after administration. Histomorphological analysis of the fatty liver grafts (microvesicle 40%) with 12 hrs cold preservation and 2 hrs reperfusion showed significantly less apoptotic cells (TUNEL positive), less endothelial cell activation (ICAM-1 positive), less neutrophil (myeloperoxidase positive) and macrophage (ED-1 positive) infiltration in PTD/HO-1-treated fatty liver grafts as compared to controls. In addition, electron microscopy shows a less intrahepatic bile duct damage after reperfusion in the graft treated with PTD-HO-1. Profiling the intragraft gene expression by real-time PCR showed less IL-1 and IL-6 proinflammatory gene expressions in PTD-HO-1-treated liver grafts as compared to controls. Conclusions: We demonstrated a novel method to protect fatty liver graft against I/R injury with great potential for clinical application.
Persistent Identifierhttp://hdl.handle.net/10722/172993
ISSN
2015 SCImago Journal Rankings: 0.108

 

DC FieldValueLanguage
dc.contributor.authorTsui, TYen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorMan, Ken_US
dc.contributor.authorSchlitt, HJen_US
dc.contributor.authorNashan, Ben_US
dc.date.accessioned2012-10-30T06:26:19Z-
dc.date.available2012-10-30T06:26:19Z-
dc.date.issued2009en_US
dc.identifier.citationTransplantationsmedizin: Organ Der Deutschen Transplantationsgesellschaft, 2009, v. 21 SUPPL. 2, p. 17en_US
dc.identifier.issn0946-9648en_US
dc.identifier.urihttp://hdl.handle.net/10722/172993-
dc.description.abstractBackgrounds: Fatty liver accelerates the I/R injury after reperfusion that significantly affects the outcome of liver transplantation. In the study, we established a novel protein transduction system to prevent I/R injury of fatty livers. Methods and results: Modified human heme oxygenase-1 (HO-1) or enhanced green fluorescent protein (EGFP) recombinant protein containing a cell-penetrating domain (PTD) was generated. Their biological activities were investigated in a syngeneic liver transplantation model. PTD/HO-1 or PTD/EGFP was administered through portal vein after the graft harvesting and the grafts were preserved in cold UW solution. The targeted protein was found in the vessel wall of portal areas and adjuvant liver cells at 10 min after administration. Histomorphological analysis of the fatty liver grafts (microvesicle 40%) with 12 hrs cold preservation and 2 hrs reperfusion showed significantly less apoptotic cells (TUNEL positive), less endothelial cell activation (ICAM-1 positive), less neutrophil (myeloperoxidase positive) and macrophage (ED-1 positive) infiltration in PTD/HO-1-treated fatty liver grafts as compared to controls. In addition, electron microscopy shows a less intrahepatic bile duct damage after reperfusion in the graft treated with PTD-HO-1. Profiling the intragraft gene expression by real-time PCR showed less IL-1 and IL-6 proinflammatory gene expressions in PTD-HO-1-treated liver grafts as compared to controls. Conclusions: We demonstrated a novel method to protect fatty liver graft against I/R injury with great potential for clinical application.en_US
dc.languageengen_US
dc.publisherPabst Science Publishers. The Journal's web site is located at http://www.pabst-publishers.de/txmeden_US
dc.relation.ispartofTransplantationsmedizin: Organ der Deutschen Transplantationsgesellschaften_US
dc.titleA novel approach to protect fatty liver graft against I/R injuryen_US
dc.typeArticleen_US
dc.identifier.emailMan, K: kwanman@hkucc.hku.hken_US
dc.identifier.authorityMan, K=rp00417en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.scopuseid_2-s2.0-70450067890en_US
dc.identifier.volume21en_US
dc.identifier.issueSUPPL. 2en_US
dc.identifier.spage17en_US
dc.publisher.placeGermanyen_US
dc.identifier.scopusauthoridTsui, TY=7006622455en_US
dc.identifier.scopusauthoridLi, Y=35178001700en_US
dc.identifier.scopusauthoridMan, K=7101754072en_US
dc.identifier.scopusauthoridSchlitt, HJ=7005572464en_US
dc.identifier.scopusauthoridNashan, B=35415635000en_US

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