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Article: Regulatory role of vHL/HIF-1α in hypoxia-induced VEGF production in hepatic stellate cells

TitleRegulatory role of vHL/HIF-1α in hypoxia-induced VEGF production in hepatic stellate cells
Authors
KeywordsCOX-2
HIF-1α
Hypoxic T6-HSCs
VEGF
vHL
Issue Date2004
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/description
Citation
Biochemical And Biophysical Research Communications, 2004, v. 317 n. 2, p. 358-362 How to Cite?
AbstractActivated hepatic stellate cells (HSCs) produce cyclooxygenase-2 (COX-2) protein to induce vascular endothelial growth factor (VEGF) production that participates in angiogenesis in injured liver. To reveal the unknown regulatory mechanism, we used hypoxic atmosphere mimicking injured-tissue microenvironment to induce VEGF expression in a rat hepatic stellate cell line (T6-HSCs). The present study showed that hypoxia up-regulated the protein levels of COX-2 and hypoxia-inducible factor-1-α (HIF-1α), but rapidly effected degradation of von Hippel-Lindau (vHL) protein. As a result, expression of VEGF in HSCs was markedly elevated; and pretreatment with COX-2 inhibitors (nimesulide or indomethacin) could significantly ameliorate the angiogenic event. Collectively, hypoxic HSCs increased accumulation of HIF-1α protein and induced VEGF expression in a time-dependent manner. Inhibition of COX-2 activities would prevent vHL protein from degradation and suppress HIF-1α up-regulation. Thus, vHL/HIF-1α has a regulatory role in COX-2-mediated VEGF production in hypoxic stellate cells in injured liver. © 2004 Elsevier Inc. All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/172870
ISSN
2015 Impact Factor: 2.371
2015 SCImago Journal Rankings: 1.152
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWang, YQen_HK
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorIkeda, Ken_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorChu, ACen_HK
dc.contributor.authorKaneda, Ken_HK
dc.contributor.authorFan, STen_HK
dc.date.accessioned2012-10-30T06:25:25Z-
dc.date.available2012-10-30T06:25:25Z-
dc.date.issued2004en_HK
dc.identifier.citationBiochemical And Biophysical Research Communications, 2004, v. 317 n. 2, p. 358-362en_HK
dc.identifier.issn0006-291Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/172870-
dc.description.abstractActivated hepatic stellate cells (HSCs) produce cyclooxygenase-2 (COX-2) protein to induce vascular endothelial growth factor (VEGF) production that participates in angiogenesis in injured liver. To reveal the unknown regulatory mechanism, we used hypoxic atmosphere mimicking injured-tissue microenvironment to induce VEGF expression in a rat hepatic stellate cell line (T6-HSCs). The present study showed that hypoxia up-regulated the protein levels of COX-2 and hypoxia-inducible factor-1-α (HIF-1α), but rapidly effected degradation of von Hippel-Lindau (vHL) protein. As a result, expression of VEGF in HSCs was markedly elevated; and pretreatment with COX-2 inhibitors (nimesulide or indomethacin) could significantly ameliorate the angiogenic event. Collectively, hypoxic HSCs increased accumulation of HIF-1α protein and induced VEGF expression in a time-dependent manner. Inhibition of COX-2 activities would prevent vHL protein from degradation and suppress HIF-1α up-regulation. Thus, vHL/HIF-1α has a regulatory role in COX-2-mediated VEGF production in hypoxic stellate cells in injured liver. © 2004 Elsevier Inc. All rights reserved.en_HK
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/wps/find/journaldescription.cws_home/622790/descriptionen_HK
dc.relation.ispartofBiochemical and Biophysical Research Communicationsen_HK
dc.subjectCOX-2en_HK
dc.subjectHIF-1αen_HK
dc.subjectHypoxic T6-HSCsen_HK
dc.subjectVEGFen_HK
dc.subjectvHLen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshCarrier Proteins - Metabolismen_US
dc.subject.meshCell Hypoxia - Drug Effects - Physiologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCyclooxygenase 2en_US
dc.subject.meshDose-Response Relationship, Drugen_US
dc.subject.meshGene Expression Regulation - Drug Effects - Physiologyen_US
dc.subject.meshHepatocytes - Drug Effects - Metabolismen_US
dc.subject.meshHomeostasis - Physiologyen_US
dc.subject.meshHypoxia-Inducible Factor 1, Alpha Subuniten_US
dc.subject.meshIndomethacin - Pharmacologyen_US
dc.subject.meshIsoenzymes - Antagonists & Inhibitors - Metabolismen_US
dc.subject.meshProstaglandin-Endoperoxide Synthases - Metabolismen_US
dc.subject.meshRatsen_US
dc.subject.meshSulfonamides - Pharmacologyen_US
dc.subject.meshTranscription Factors - Metabolismen_US
dc.subject.meshVascular Endothelial Growth Factor A - Metabolismen_US
dc.titleRegulatory role of vHL/HIF-1α in hypoxia-induced VEGF production in hepatic stellate cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.emailChu, AC: bcccy@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityChu, AC=rp00505en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/j.bbrc.2004.03.050en_HK
dc.identifier.pmid15063765-
dc.identifier.scopuseid_2-s2.0-1842426689en_HK
dc.identifier.hkuros85943-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-1842426689&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume317en_HK
dc.identifier.issue2en_HK
dc.identifier.spage358en_HK
dc.identifier.epage362en_HK
dc.identifier.isiWOS:000220922200009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridWang, YQ=37074971500en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridIkeda, K=7404890758en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridChu, AC=24343085700en_HK
dc.identifier.scopusauthoridKaneda, K=7202540217en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK

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