Article: Induction of MCP1, CCR2, and iNOS expression in THP-1 macrophages by serum of children late after kawasaki disease

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TitleInduction of MCP1, CCR2, and iNOS expression in THP-1 macrophages by serum of children late after kawasaki disease
AuthorsCheung, YF2
Karmin, O1
Tam, SCF3
Siow, YL1
Issue Date2005
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/
CitationPediatric Research, 2005, v. 58 n. 6, p. 1306-1310 [How to Cite?]
DOI: http://dx.doi.org/10.1203/01.pdr.0000183360.79872.1c
AbstractEvidence of premature atherosclerosis late after Kawasaki disease (KD) is accumulating. Given the potential roles of monocyte chemoattractant protein-1 (MCP-1), chemokine receptor CCR-2, and inducible nitric oxide synthase (iNOS) in atherogenesis, we sought to determine whether serum obtained from children late after KD would induce expression of these genes in macrophages in vitro. A total of 79 subjects were studied, which comprised 57 KD patients, 33 of whom had coronary aneurysms, and 22 age-matched controls. Expression of MCP-1, CCR2, and iNOS mRNA in THP-1 macrophages in the presence of patient and control serum was quantified as a ratio to β-actin mRNA and expressed as a percentage of control. MCP-1 expression was significantly increased in the presence of serum from patients with coronary aneurysms. Expression of CCR2 and iNOS was significantly increased when THP-1 macrophages were incubated with serum from patients with and without coronary aneurysms. The magnitude of induction of MCP-1, CCR2, and iNOS or in combinations correlated positively with serum high-sensitivity C-reactive protein (hs-CRP), and low-density lipoprotein (LDL) cholesterol levels and negatively with high-density lipoprotein (HDL) cholesterol level. In conclusion, the serum of patients with a history of KD induces expression of MCP-1, CCR2, and iNOS in THP-1 macrophages in vitro. Induction of these genes in vivo may be related to chronic inflammation and may have important implications for premature atherosclerosis. Copyright © 2005 International Pediatric Research Foundation, Inc.
ISSN0031-3998
2011 Impact Factor: 2.7
2011 SCImago Journal Rankings: 0.238
DOIhttp://dx.doi.org/10.1203/01.pdr.0000183360.79872.1c
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorCheung, YF
dc.contributor.authorKarmin, O
dc.contributor.authorTam, SCF
dc.contributor.authorSiow, YL
dc.date.accessioned2012-10-30T06:07:44Z
dc.date.available2012-10-30T06:07:44Z
dc.date.issued2005
dc.description.abstractEvidence of premature atherosclerosis late after Kawasaki disease (KD) is accumulating. Given the potential roles of monocyte chemoattractant protein-1 (MCP-1), chemokine receptor CCR-2, and inducible nitric oxide synthase (iNOS) in atherogenesis, we sought to determine whether serum obtained from children late after KD would induce expression of these genes in macrophages in vitro. A total of 79 subjects were studied, which comprised 57 KD patients, 33 of whom had coronary aneurysms, and 22 age-matched controls. Expression of MCP-1, CCR2, and iNOS mRNA in THP-1 macrophages in the presence of patient and control serum was quantified as a ratio to β-actin mRNA and expressed as a percentage of control. MCP-1 expression was significantly increased in the presence of serum from patients with coronary aneurysms. Expression of CCR2 and iNOS was significantly increased when THP-1 macrophages were incubated with serum from patients with and without coronary aneurysms. The magnitude of induction of MCP-1, CCR2, and iNOS or in combinations correlated positively with serum high-sensitivity C-reactive protein (hs-CRP), and low-density lipoprotein (LDL) cholesterol levels and negatively with high-density lipoprotein (HDL) cholesterol level. In conclusion, the serum of patients with a history of KD induces expression of MCP-1, CCR2, and iNOS in THP-1 macrophages in vitro. Induction of these genes in vivo may be related to chronic inflammation and may have important implications for premature atherosclerosis. Copyright © 2005 International Pediatric Research Foundation, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationPediatric Research, 2005, v. 58 n. 6, p. 1306-1310 [How to Cite?]
DOI: http://dx.doi.org/10.1203/01.pdr.0000183360.79872.1c
dc.identifier.doihttp://dx.doi.org/10.1203/01.pdr.0000183360.79872.1c
dc.identifier.epage1310
dc.identifier.issn0031-3998
2011 Impact Factor: 2.7
2011 SCImago Journal Rankings: 0.238
dc.identifier.issue6
dc.identifier.pmid16306213
dc.identifier.scopuseid_2-s2.0-27644468219
dc.identifier.spage1306
dc.identifier.urihttp://hdl.handle.net/10722/170352
dc.identifier.volume58
dc.languageeng
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/
dc.publisher.placeUnited States
dc.relation.ispartofPediatric Research
dc.relation.referencesReferences in Scopus
dc.subject.meshAtherosclerosis - Genetics
dc.subject.meshCells, Cultured
dc.subject.meshChemokine Ccl2 - Genetics
dc.subject.meshChild
dc.subject.meshFemale
dc.subject.meshGene Expression
dc.subject.meshHumans
dc.subject.meshLipids - Blood
dc.subject.meshMacrophages - Metabolism
dc.subject.meshMale
dc.subject.meshMucocutaneous Lymph Node Syndrome - Complications - Genetics - Immunology
dc.subject.meshNitric Oxide Synthase Type Ii - Genetics
dc.subject.meshReceptors, Ccr2
dc.subject.meshReceptors, Chemokine - Genetics
dc.subject.meshSerum - Metabolism
dc.subject.meshUp-Regulation - Genetics
dc.titleInduction of MCP1, CCR2, and iNOS expression in THP-1 macrophages by serum of children late after kawasaki disease
dc.typeArticle
Author Affiliations
  1. University of Manitoba
  2. The University of Hong Kong
  3. Queen Mary Hospital Hong Kong