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Article: Induction of MCP1, CCR2, and iNOS expression in THP-1 macrophages by serum of children late after kawasaki disease
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TitleInduction of MCP1, CCR2, and iNOS expression in THP-1 macrophages by serum of children late after kawasaki disease
 
AuthorsCheung, YF2
Karmin, O1
Tam, SCF3
Siow, YL1
 
Issue Date2005
 
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/
 
CitationPediatric Research, 2005, v. 58 n. 6, p. 1306-1310 [How to Cite?]
DOI: http://dx.doi.org/10.1203/01.pdr.0000183360.79872.1c
 
AbstractEvidence of premature atherosclerosis late after Kawasaki disease (KD) is accumulating. Given the potential roles of monocyte chemoattractant protein-1 (MCP-1), chemokine receptor CCR-2, and inducible nitric oxide synthase (iNOS) in atherogenesis, we sought to determine whether serum obtained from children late after KD would induce expression of these genes in macrophages in vitro. A total of 79 subjects were studied, which comprised 57 KD patients, 33 of whom had coronary aneurysms, and 22 age-matched controls. Expression of MCP-1, CCR2, and iNOS mRNA in THP-1 macrophages in the presence of patient and control serum was quantified as a ratio to β-actin mRNA and expressed as a percentage of control. MCP-1 expression was significantly increased in the presence of serum from patients with coronary aneurysms. Expression of CCR2 and iNOS was significantly increased when THP-1 macrophages were incubated with serum from patients with and without coronary aneurysms. The magnitude of induction of MCP-1, CCR2, and iNOS or in combinations correlated positively with serum high-sensitivity C-reactive protein (hs-CRP), and low-density lipoprotein (LDL) cholesterol levels and negatively with high-density lipoprotein (HDL) cholesterol level. In conclusion, the serum of patients with a history of KD induces expression of MCP-1, CCR2, and iNOS in THP-1 macrophages in vitro. Induction of these genes in vivo may be related to chronic inflammation and may have important implications for premature atherosclerosis. Copyright © 2005 International Pediatric Research Foundation, Inc.
 
ISSN0031-3998
2012 Impact Factor: 2.673
2012 SCImago Journal Rankings: 1.153
 
DOIhttp://dx.doi.org/10.1203/01.pdr.0000183360.79872.1c
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorCheung, YF
 
dc.contributor.authorKarmin, O
 
dc.contributor.authorTam, SCF
 
dc.contributor.authorSiow, YL
 
dc.date.accessioned2012-10-30T06:07:44Z
 
dc.date.available2012-10-30T06:07:44Z
 
dc.date.issued2005
 
dc.description.abstractEvidence of premature atherosclerosis late after Kawasaki disease (KD) is accumulating. Given the potential roles of monocyte chemoattractant protein-1 (MCP-1), chemokine receptor CCR-2, and inducible nitric oxide synthase (iNOS) in atherogenesis, we sought to determine whether serum obtained from children late after KD would induce expression of these genes in macrophages in vitro. A total of 79 subjects were studied, which comprised 57 KD patients, 33 of whom had coronary aneurysms, and 22 age-matched controls. Expression of MCP-1, CCR2, and iNOS mRNA in THP-1 macrophages in the presence of patient and control serum was quantified as a ratio to β-actin mRNA and expressed as a percentage of control. MCP-1 expression was significantly increased in the presence of serum from patients with coronary aneurysms. Expression of CCR2 and iNOS was significantly increased when THP-1 macrophages were incubated with serum from patients with and without coronary aneurysms. The magnitude of induction of MCP-1, CCR2, and iNOS or in combinations correlated positively with serum high-sensitivity C-reactive protein (hs-CRP), and low-density lipoprotein (LDL) cholesterol levels and negatively with high-density lipoprotein (HDL) cholesterol level. In conclusion, the serum of patients with a history of KD induces expression of MCP-1, CCR2, and iNOS in THP-1 macrophages in vitro. Induction of these genes in vivo may be related to chronic inflammation and may have important implications for premature atherosclerosis. Copyright © 2005 International Pediatric Research Foundation, Inc.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationPediatric Research, 2005, v. 58 n. 6, p. 1306-1310 [How to Cite?]
DOI: http://dx.doi.org/10.1203/01.pdr.0000183360.79872.1c
 
dc.identifier.doihttp://dx.doi.org/10.1203/01.pdr.0000183360.79872.1c
 
dc.identifier.epage1310
 
dc.identifier.issn0031-3998
2012 Impact Factor: 2.673
2012 SCImago Journal Rankings: 1.153
 
dc.identifier.issue6
 
dc.identifier.pmid16306213
 
dc.identifier.scopuseid_2-s2.0-27644468219
 
dc.identifier.spage1306
 
dc.identifier.urihttp://hdl.handle.net/10722/170352
 
dc.identifier.volume58
 
dc.languageeng
 
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.pedresearch.org/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofPediatric Research
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshAtherosclerosis - Genetics
 
dc.subject.meshCells, Cultured
 
dc.subject.meshChemokine Ccl2 - Genetics
 
dc.subject.meshChild
 
dc.subject.meshFemale
 
dc.subject.meshGene Expression
 
dc.subject.meshHumans
 
dc.subject.meshLipids - Blood
 
dc.subject.meshMacrophages - Metabolism
 
dc.subject.meshMale
 
dc.subject.meshMucocutaneous Lymph Node Syndrome - Complications - Genetics - Immunology
 
dc.subject.meshNitric Oxide Synthase Type Ii - Genetics
 
dc.subject.meshReceptors, Ccr2
 
dc.subject.meshReceptors, Chemokine - Genetics
 
dc.subject.meshSerum - Metabolism
 
dc.subject.meshUp-Regulation - Genetics
 
dc.titleInduction of MCP1, CCR2, and iNOS expression in THP-1 macrophages by serum of children late after kawasaki disease
 
dc.typeArticle
 
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<description.abstract>Evidence of premature atherosclerosis late after Kawasaki disease (KD) is accumulating. Given the potential roles of monocyte chemoattractant protein-1 (MCP-1), chemokine receptor CCR-2, and inducible nitric oxide synthase (iNOS) in atherogenesis, we sought to determine whether serum obtained from children late after KD would induce expression of these genes in macrophages in vitro. A total of 79 subjects were studied, which comprised 57 KD patients, 33 of whom had coronary aneurysms, and 22 age-matched controls. Expression of MCP-1, CCR2, and iNOS mRNA in THP-1 macrophages in the presence of patient and control serum was quantified as a ratio to &#946;-actin mRNA and expressed as a percentage of control. MCP-1 expression was significantly increased in the presence of serum from patients with coronary aneurysms. Expression of CCR2 and iNOS was significantly increased when THP-1 macrophages were incubated with serum from patients with and without coronary aneurysms. The magnitude of induction of MCP-1, CCR2, and iNOS or in combinations correlated positively with serum high-sensitivity C-reactive protein (hs-CRP), and low-density lipoprotein (LDL) cholesterol levels and negatively with high-density lipoprotein (HDL) cholesterol level. In conclusion, the serum of patients with a history of KD induces expression of MCP-1, CCR2, and iNOS in THP-1 macrophages in vitro. Induction of these genes in vivo may be related to chronic inflammation and may have important implications for premature atherosclerosis. Copyright &#169; 2005 International Pediatric Research Foundation, Inc.</description.abstract>
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Author Affiliations
  1. University of Manitoba
  2. The University of Hong Kong
  3. Queen Mary Hospital Hong Kong