File Download

Conference Paper: Derivation of oligodendrocyte precursors from bone marrow stromal cells for mylination therapy

TitleDerivation of oligodendrocyte precursors from bone marrow stromal cells for mylination therapy
Authors
Issue Date2012
PublisherHKSN & BPHK.
Citation
The Hong Kong-Taiwan Physiology Symposium 2012 and Joint Scientific Meeting of Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book of the Joint Scientific Meeting, 2012, p. 72, abstract P56 How to Cite?
Abstract
Myelin damage and disorders caused by physical damage or diseases like leukodystrophies result in severe loss of function. With an aim towards remyelination therapy, we attempted to direct differentiation of bone marrow stromal cells (BMSCs, adult rats) along the oligodendroglial lineage in vitro. BMSCs were first cultured as non-adherent spheres until they expressed markers of neural/glial progenitors. The neural/glial progenitors were then maintained in adherent culture supplemented with β-heregulin, PDGF-AA and bFGF. Oligodendrocyte precursors expressing the markers - NG2, Olig2, PDGFRa and Sox10, were detectable within two weeks and can be expanded in culture for up to 3 months with no observable decline in marker expression. To test for myelination capability, BMSC-derived oligodendrocyte precursor cells (OPCs) in a 2-week co-culture with dorsal root ganglion neurons extended myelin basic protein-positive processes along neurites, suggesting maturation into myelinating oligodendrocytes. In vivo myelination by BM-OPCs was tested by exploitation of unmyelinated axons of retinal ganglion cells of adult rats. Myelin basic protein-positive processes were also observable along retinal axons by 8 weeks post-injection. Our findings indicate BMSCs as a possible source of OPCs for remyelination therapy.
DescriptionPoster Presentation: P56
Persistent Identifierhttp://hdl.handle.net/10722/166826

 

DC FieldValueLanguage
dc.contributor.authorTsui, YPen_US
dc.contributor.authorLi, RSYen_US
dc.contributor.authorLo, ACYen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2012-09-20T08:50:24Z-
dc.date.available2012-09-20T08:50:24Z-
dc.date.issued2012en_US
dc.identifier.citationThe Hong Kong-Taiwan Physiology Symposium 2012 and Joint Scientific Meeting of Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book of the Joint Scientific Meeting, 2012, p. 72, abstract P56en_US
dc.identifier.urihttp://hdl.handle.net/10722/166826-
dc.descriptionPoster Presentation: P56-
dc.description.abstractMyelin damage and disorders caused by physical damage or diseases like leukodystrophies result in severe loss of function. With an aim towards remyelination therapy, we attempted to direct differentiation of bone marrow stromal cells (BMSCs, adult rats) along the oligodendroglial lineage in vitro. BMSCs were first cultured as non-adherent spheres until they expressed markers of neural/glial progenitors. The neural/glial progenitors were then maintained in adherent culture supplemented with β-heregulin, PDGF-AA and bFGF. Oligodendrocyte precursors expressing the markers - NG2, Olig2, PDGFRa and Sox10, were detectable within two weeks and can be expanded in culture for up to 3 months with no observable decline in marker expression. To test for myelination capability, BMSC-derived oligodendrocyte precursor cells (OPCs) in a 2-week co-culture with dorsal root ganglion neurons extended myelin basic protein-positive processes along neurites, suggesting maturation into myelinating oligodendrocytes. In vivo myelination by BM-OPCs was tested by exploitation of unmyelinated axons of retinal ganglion cells of adult rats. Myelin basic protein-positive processes were also observable along retinal axons by 8 weeks post-injection. Our findings indicate BMSCs as a possible source of OPCs for remyelination therapy.-
dc.languageengen_US
dc.publisherHKSN & BPHK.-
dc.relation.ispartofProgram Book of the Joint Scientific Meetingen_US
dc.titleDerivation of oligodendrocyte precursors from bone marrow stromal cells for mylination therapyen_US
dc.typeConference_Paperen_US
dc.identifier.emailTsui, YP: h0694071@hku.hken_US
dc.identifier.emailLi, RSY: bobli@ie.cuhk.edu.hken_US
dc.identifier.emailLo, ACY: amylo@hkucc.hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hk-
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hk-
dc.identifier.authorityLo, ACY=rp00425en_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros209403en_US
dc.identifier.spage72-
dc.identifier.epage72-
dc.publisher.placeHong Kong-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats