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Conference Paper: Seeing is believing; tracking metalloproteins by fluorescent probe in vivo and in vitro

TitleSeeing is believing; tracking metalloproteins by fluorescent probe in vivo and in vitro
Authors
Issue Date2012
Citation
The 12th EuroAsia Conference on Chemical Sciences (EuAsC2S-12), Corfu, Greece, 16-21 April 2012. How to Cite?
AbstractExtensive genome research has shown that around 1/4 to 1/3 proteins are metalloproteins (or metal-binding proteins) with various metal ions incorporated with proteins for either structural or functional purposes. Thus, metalloproteomics/metallomics are developed to investigate the molecular mechanism of metal-related biological processes and the entirety of metal/metalloid species within a cell or tissue type[1]. Fluorescence labeling is probably the best method in view of its capability in providing rapid and sensitive identification in living biological systems. In spite of the development of fluorescent proteins, synthetic small-molecule fluorescence agents have been utilized to identify specific targets in cells, while metal-chelation methodology has been extensively applied to the study of metal-oriented biological process[2]. Although different types of metal-responsive sensors have been developed to label cellular metals[3], tracking of metal-binding proteins in living cells by fluorescence is still highly anticipated. In this work, novel fluorescent probe was designed to label metalloproteins both in vivo and in vitro. The protein partners of several metal ions such as Ni2+ (Histidine-rich proteins in particular), Bi3+, Cr3+ have been identified by the agent. The fluorescent agent exhibited “turnon” response to the targets in SDS-PAGE, and its excellent permeability enabled “lighting up” of targeted proteins in living cells, providing valuable information on metalloprotein spatial distribution in biology.
DescriptionAbstract no. EuAsC2S-12/S1-OP22
Persistent Identifierhttp://hdl.handle.net/10722/165124

 

DC FieldValueLanguage
dc.contributor.authorLai, YTen_US
dc.contributor.authorHu, Len_US
dc.contributor.authorCheng, Ten_US
dc.contributor.authorXia, Wen_US
dc.contributor.authorSun, Hen_US
dc.date.accessioned2012-09-20T08:15:07Z-
dc.date.available2012-09-20T08:15:07Z-
dc.date.issued2012en_US
dc.identifier.citationThe 12th EuroAsia Conference on Chemical Sciences (EuAsC2S-12), Corfu, Greece, 16-21 April 2012.en_US
dc.identifier.urihttp://hdl.handle.net/10722/165124-
dc.descriptionAbstract no. EuAsC2S-12/S1-OP22-
dc.description.abstractExtensive genome research has shown that around 1/4 to 1/3 proteins are metalloproteins (or metal-binding proteins) with various metal ions incorporated with proteins for either structural or functional purposes. Thus, metalloproteomics/metallomics are developed to investigate the molecular mechanism of metal-related biological processes and the entirety of metal/metalloid species within a cell or tissue type[1]. Fluorescence labeling is probably the best method in view of its capability in providing rapid and sensitive identification in living biological systems. In spite of the development of fluorescent proteins, synthetic small-molecule fluorescence agents have been utilized to identify specific targets in cells, while metal-chelation methodology has been extensively applied to the study of metal-oriented biological process[2]. Although different types of metal-responsive sensors have been developed to label cellular metals[3], tracking of metal-binding proteins in living cells by fluorescence is still highly anticipated. In this work, novel fluorescent probe was designed to label metalloproteins both in vivo and in vitro. The protein partners of several metal ions such as Ni2+ (Histidine-rich proteins in particular), Bi3+, Cr3+ have been identified by the agent. The fluorescent agent exhibited “turnon” response to the targets in SDS-PAGE, and its excellent permeability enabled “lighting up” of targeted proteins in living cells, providing valuable information on metalloprotein spatial distribution in biology.-
dc.languageengen_US
dc.relation.ispartofEuroAsia Conference on Chemical Sciences, EuAsC2S-12en_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleSeeing is believing; tracking metalloproteins by fluorescent probe in vivo and in vitroen_US
dc.typeConference_Paperen_US
dc.identifier.emailLai, YT: skytchk@hku.hken_US
dc.identifier.emailHu, L: liganghu@hku.hken_US
dc.identifier.emailCheng, T: tfccheng@hku.hk-
dc.identifier.emailXia, W: h0692113@hku.hk-
dc.identifier.emailSun, H: hsun@hku.hk-
dc.identifier.authoritySun, H=rp00777en_US
dc.description.naturepostprint-
dc.identifier.hkuros211155en_US
dc.description.otherThe 12th EuroAsia Conference on Chemical Sciences (EuAsC2S-12), Corfu, Greece, 16-21 April 2012.-

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