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Conference Paper: Preparation of chitosan fibers for nerve regeneration

TitlePreparation of chitosan fibers for nerve regeneration
Authors
Issue Date2012
PublisherHKSN & BPHK.
Citation
The Hong Kong-Taiwan Physiology Symposium 2012 and Joint Scientific Meeting of Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book of the Joint Scientific Meeting, 2012, p. 75, abstract P63 How to Cite?
AbstractSchwann cells have been exploited to guide axonal regrowth after nerve injury. However, mis-orientation of Schwann cells in nerve guidance channels can hinder axonal growth across the lesion site. We therefore propose to orient the growth of Schwann cells on uniaxially aligned fibres such that axonal growth can be guided along the designated direction towards the target. Chitosan was the choice scaffold material given its biocompatibility and the tunable susceptibility to biodegradation. With a conventional electrospinning setup and chitosan dissolved in trifluoroacetic acid/methylene chloride, our initial attempts resulted in a random orientation of chitosan fibers at the cathodic collector. By replacing the grounded plate collector with parallel collector plates placed 1.6 cm apart, the positively charged chitosan fibers became alternately attracted to the parallel plates and ended up uniaxially aligned as fibre suspension across the plates. Stability of the chitosan fibers in aqueous, physiological environment was achieved with use of sodium carbonate to neutralize residual acidity in the chitosan fiber preparation. Cell culture experiments are ongoing to test if Schwann cell behavior varies with fiber thickness of the stabilized chitosan fiber preparations.
DescriptionPoster Presentation: P63
Persistent Identifierhttp://hdl.handle.net/10722/165085

 

DC FieldValueLanguage
dc.contributor.authorTung, WTen_US
dc.contributor.authorTam, KWen_US
dc.contributor.authorChan, YSen_US
dc.contributor.authorShum, DKYen_US
dc.date.accessioned2012-09-20T08:14:41Z-
dc.date.available2012-09-20T08:14:41Z-
dc.date.issued2012en_US
dc.identifier.citationThe Hong Kong-Taiwan Physiology Symposium 2012 and Joint Scientific Meeting of Hong Kong Society of Neurosciences & The Biophysical Society of Hong Kong, Hong Kong, 14-15 June 2012. In Program Book of the Joint Scientific Meeting, 2012, p. 75, abstract P63en_US
dc.identifier.urihttp://hdl.handle.net/10722/165085-
dc.descriptionPoster Presentation: P63-
dc.description.abstractSchwann cells have been exploited to guide axonal regrowth after nerve injury. However, mis-orientation of Schwann cells in nerve guidance channels can hinder axonal growth across the lesion site. We therefore propose to orient the growth of Schwann cells on uniaxially aligned fibres such that axonal growth can be guided along the designated direction towards the target. Chitosan was the choice scaffold material given its biocompatibility and the tunable susceptibility to biodegradation. With a conventional electrospinning setup and chitosan dissolved in trifluoroacetic acid/methylene chloride, our initial attempts resulted in a random orientation of chitosan fibers at the cathodic collector. By replacing the grounded plate collector with parallel collector plates placed 1.6 cm apart, the positively charged chitosan fibers became alternately attracted to the parallel plates and ended up uniaxially aligned as fibre suspension across the plates. Stability of the chitosan fibers in aqueous, physiological environment was achieved with use of sodium carbonate to neutralize residual acidity in the chitosan fiber preparation. Cell culture experiments are ongoing to test if Schwann cell behavior varies with fiber thickness of the stabilized chitosan fiber preparations.-
dc.languageengen_US
dc.publisherHKSN & BPHK.-
dc.relation.ispartofProgram Book of the Joint Scientific Meetingen_US
dc.titlePreparation of chitosan fibers for nerve regenerationen_US
dc.typeConference_Paperen_US
dc.identifier.emailTung, WT: wingtaii@hku.hken_US
dc.identifier.emailTam, KW: tamkw@hku.hken_US
dc.identifier.emailChan, YS: yschan@hku.hken_US
dc.identifier.emailShum, DKY: shumdkhk@hkucc.hku.hken_US
dc.identifier.authorityChan, YS=rp00318en_US
dc.identifier.authorityShum, DKY=rp00321en_US
dc.description.naturelink_to_OA_fulltext-
dc.identifier.hkuros200593en_US
dc.identifier.hkuros209442-
dc.identifier.spage75-
dc.identifier.epage75-
dc.publisher.placeHong Kong-

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