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Article: Enhanced apoptosis and senescence of bone-marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus

TitleEnhanced apoptosis and senescence of bone-marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus
Authors
Issue Date2012
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/jht
Citation
Stem Cells and Development, 2012, v. 21 n. 13, p. 2387-2394 How to Cite?
AbstractPrevious studies indicated that bone marrow mesenchymal stem cells (BMSCs) from patients with systemic lupus erythematosus (SLE) exhibited impaired capacities of proliferation, differentiation, secretion of cytokines, and immune modulation. In this study, we aimed to investigate whether apoptosis and senescence of SLE BMSCs were dysregulated. We found that there were increased frequencies of apoptotic and aging SLE BMSCs in comparison with those of normal controls. Notably, levels of Bcl-2 expression in SLE BMSCs were markedly decreased both at mRNA and protein levels. When BMSCs were induced to apoptosis by tumor necrosis factor-α (TNF-α) stimulation in vitro, the Bax and caspase 8 expression in SLE BMSCs was significantly increased at mRNA levels. The activity of caspase 8 was also enhanced in SLE BMSCs. More cytochrome-C-positive pellets in the cytosolic fraction of BMSCs were detected in SLE patients than in normal controls. The expression of Fas and tumor necrosis factor-α receptor 1 in SLE BMSCs was significantly upregulated compared with normal controls, and the serum levels of FasL and TNF-α were also elevated. Moreover, intracellular reactive oxygen species levels of SLE BMSCs were higher than those of normal controls, with the activation of PI3K/AKT/FoxO3 signaling pathway. Taken together, our results demonstrate increased apoptosis and senescence in SLE BMSCs, which may be associated with the pathogenesis of SLE.
Persistent Identifierhttp://hdl.handle.net/10722/164850
ISSN
2017 Impact Factor: 3.315
2015 SCImago Journal Rankings: 1.703
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLi, X-
dc.contributor.authorLiu, L-
dc.contributor.authorMeng, D-
dc.contributor.authorWang, D-
dc.contributor.authorZhang, J-
dc.contributor.authorShi, D-
dc.contributor.authorLiu, H-
dc.contributor.authorXu, H-
dc.contributor.authorLu, L-
dc.contributor.authorSun, L-
dc.date.accessioned2012-09-20T08:10:58Z-
dc.date.available2012-09-20T08:10:58Z-
dc.date.issued2012-
dc.identifier.citationStem Cells and Development, 2012, v. 21 n. 13, p. 2387-2394-
dc.identifier.issn1547-3287-
dc.identifier.urihttp://hdl.handle.net/10722/164850-
dc.description.abstractPrevious studies indicated that bone marrow mesenchymal stem cells (BMSCs) from patients with systemic lupus erythematosus (SLE) exhibited impaired capacities of proliferation, differentiation, secretion of cytokines, and immune modulation. In this study, we aimed to investigate whether apoptosis and senescence of SLE BMSCs were dysregulated. We found that there were increased frequencies of apoptotic and aging SLE BMSCs in comparison with those of normal controls. Notably, levels of Bcl-2 expression in SLE BMSCs were markedly decreased both at mRNA and protein levels. When BMSCs were induced to apoptosis by tumor necrosis factor-α (TNF-α) stimulation in vitro, the Bax and caspase 8 expression in SLE BMSCs was significantly increased at mRNA levels. The activity of caspase 8 was also enhanced in SLE BMSCs. More cytochrome-C-positive pellets in the cytosolic fraction of BMSCs were detected in SLE patients than in normal controls. The expression of Fas and tumor necrosis factor-α receptor 1 in SLE BMSCs was significantly upregulated compared with normal controls, and the serum levels of FasL and TNF-α were also elevated. Moreover, intracellular reactive oxygen species levels of SLE BMSCs were higher than those of normal controls, with the activation of PI3K/AKT/FoxO3 signaling pathway. Taken together, our results demonstrate increased apoptosis and senescence in SLE BMSCs, which may be associated with the pathogenesis of SLE.-
dc.languageeng-
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/jht-
dc.relation.ispartofStem Cells and Development-
dc.rightsStem Cells and Development. Copyright © Mary Ann Liebert, Inc Publishers.-
dc.rightsFinal publication is available from Mary Ann Liebert, Inc., publishers http://dx.doi.org/[insert DOI]-
dc.titleEnhanced apoptosis and senescence of bone-marrow-derived mesenchymal stem cells in patients with systemic lupus erythematosus-
dc.typeArticle-
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1557-8534 (Electronic) 1547-3287 (Linkin&volume=&spage=&epage=&date=2012&atitle=Enhanced+Apoptosis+and+Senescence+of+Bone-Marrow-Derived+Mesenchymal+Stem+Cells+in+Patients+with+Systemic+Lupus+Erythematosusen_US
dc.identifier.emailLu, L: liweilu@hkucc.hku.hk-
dc.identifier.authorityLu, L=rp00477-
dc.description.naturepostprint-
dc.identifier.doi10.1089/scd.2011.0447-
dc.identifier.pmid22375903-
dc.identifier.scopuseid_2-s2.0-84865722353-
dc.identifier.hkuros208189-
dc.identifier.volume21-
dc.identifier.issue13-
dc.identifier.spage2387-
dc.identifier.epage2394-
dc.identifier.isiWOS:000308264900006-
dc.publisher.placeUnited States-

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