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Article: Regulatory dendritic cells program B cells to differentiate into CD19hiFcγIIbhi regulatory B cells through IFN-β and CD40L

TitleRegulatory dendritic cells program B cells to differentiate into CD19hiFcγIIbhi regulatory B cells through IFN-β and CD40L
Authors
Issue Date2012
PublisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/
Citation
Blood, 2012, v. 120 n. 3, p. 581-591 How to Cite?
Abstract
Regulatory dendritic cells (DCs) play important roles in the induction of peripheral tolerance and control of adaptive immune response. Our previous studies demonstrate that splenic stroma can drive mature DCs to proliferate and further differentiate into a unique subset of CD11b(hi)Ia(low) regulatory DCs, which could inhibit T-cell response, program generation of immunosuppressive memory CD4 T cells. However, the effect of regulatory DCs on B-cell function remains unclear. Here, we report that regulatory DCs can induce splenic B cells to differentiate into a distinct subtype of IL-10-producing regulatory B cells with unique phenotype CD19(hi)FcgammaIIb(hi). CD19(hi)FcgammaIIb(hi) B cells inhibit CD4 T-cell response via IL-10. CD19(hi)FcgammaIIb(hi) B cells have enhanced phagocytic capacity compared with conventional CD19(+) B cells, and FcgammaRIIb mediates the uptake of immune complex by CD19(hi)FcgammaIIb(hi) B cells. We found that regulatory DC-derived IFN-beta and CD40 ligand are responsible for the differentiation of CD19(hi)FcgammaIIb(hi) B cells. Furthermore, an in vivo counterpart of CD19(hi)FcgammaIIb(hi) B cells in the spleen and lymph nodes with similar phenotype and regulatory function has been identified. Our results demonstrate a new manner for regulatory DCs to down-regulate immune response by, at least partially, programming B cells into regulatory B cells.
Persistent Identifierhttp://hdl.handle.net/10722/164844
ISSN
2013 Impact Factor: 9.775
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorQian, Len_US
dc.contributor.authorQian, Cen_US
dc.contributor.authorChen, Yen_US
dc.contributor.authorBai, Yen_US
dc.contributor.authorBao, Yen_US
dc.contributor.authorLu, Len_US
dc.contributor.authorCao, Xen_US
dc.date.accessioned2012-09-20T08:10:49Z-
dc.date.available2012-09-20T08:10:49Z-
dc.date.issued2012en_US
dc.identifier.citationBlood, 2012, v. 120 n. 3, p. 581-591en_US
dc.identifier.issn0006-4971en_US
dc.identifier.urihttp://hdl.handle.net/10722/164844-
dc.description.abstractRegulatory dendritic cells (DCs) play important roles in the induction of peripheral tolerance and control of adaptive immune response. Our previous studies demonstrate that splenic stroma can drive mature DCs to proliferate and further differentiate into a unique subset of CD11b(hi)Ia(low) regulatory DCs, which could inhibit T-cell response, program generation of immunosuppressive memory CD4 T cells. However, the effect of regulatory DCs on B-cell function remains unclear. Here, we report that regulatory DCs can induce splenic B cells to differentiate into a distinct subtype of IL-10-producing regulatory B cells with unique phenotype CD19(hi)FcgammaIIb(hi). CD19(hi)FcgammaIIb(hi) B cells inhibit CD4 T-cell response via IL-10. CD19(hi)FcgammaIIb(hi) B cells have enhanced phagocytic capacity compared with conventional CD19(+) B cells, and FcgammaRIIb mediates the uptake of immune complex by CD19(hi)FcgammaIIb(hi) B cells. We found that regulatory DC-derived IFN-beta and CD40 ligand are responsible for the differentiation of CD19(hi)FcgammaIIb(hi) B cells. Furthermore, an in vivo counterpart of CD19(hi)FcgammaIIb(hi) B cells in the spleen and lymph nodes with similar phenotype and regulatory function has been identified. Our results demonstrate a new manner for regulatory DCs to down-regulate immune response by, at least partially, programming B cells into regulatory B cells.-
dc.languageengen_US
dc.publisherAmerican Society of Hematology. The Journal's web site is located at http://bloodjournal.hematologylibrary.org/-
dc.relation.ispartofBlooden_US
dc.rightsThis research was originally published in The Hematologist: ASH News and Reports. Author(s). Title. The Hematologist: ASH News and Reports. Year;Vol,Issue:pp-pp. © the American Society of Hematology.-
dc.subject.meshAntigens, CD19 - immunology - metabolism-
dc.subject.meshB-Lymphocytes - cytology - immunology - metabolism-
dc.subject.meshCD40 Ligand - immunology - metabolism-
dc.subject.meshDendritic Cells - cytology - immunology - metabolism-
dc.subject.meshInterferon-beta - immunology - metabolism-
dc.titleRegulatory dendritic cells program B cells to differentiate into CD19hiFcγIIbhi regulatory B cells through IFN-β and CD40Len_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1528-0020 (Electronic) 0006-4971 (Linkin&volume=120&issue=3&spage=581&epage=91&date=2012&atitle=Regulatory+dendritic+cells+program+B+cells+to+differentiate+into+CD19hiFcgammaIIbhi+regulatory+B+cells+through+IFN-beta+and+CD40Len_US
dc.identifier.emailLu, L: liweilu@hkucc.hku.hken_US
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1182/blood-2011-08-377242-
dc.identifier.pmid22692512-
dc.identifier.scopuseid_2-s2.0-84864126038-
dc.identifier.hkuros208182en_US
dc.identifier.volume120en_US
dc.identifier.issue3en_US
dc.identifier.spage581en_US
dc.identifier.epage591en_US
dc.identifier.isiWOS:000307440100015-
dc.publisher.placeUnited States-

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