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Article: Superior molecularly altered influenza virus hemagglutinin peptide 308-317 inhibits collagen-induced arthritis by inducing CD4+ treg cell expansion

TitleSuperior molecularly altered influenza virus hemagglutinin peptide 308-317 inhibits collagen-induced arthritis by inducing CD4+ treg cell expansion
Authors
KeywordsCollagen type 2
Gamma interferon
Influenza virus hemagglutinin[308-317]
Interleukin 10
Interleukin 6
Issue Date2012
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0004-3591/
Citation
Arthritis & Rheumatism, 2012, v. 64 n. 7, p. 2158-2168 How to Cite?
Abstract
OBJECTIVE: To investigate the inhibitory effect and possible mechanism of a novel influenza virus hemagglutinin 308-317 peptide (altered HA308-317 peptide) in collagen-induced arthritis (CIA). METHODS: CIA was induced in DBA/1 mice by immunization with type II collagen (CII). Altered HA308-317 peptide, wild HA308-317 peptide, wild CII263-272 peptide, and irrelevant peptide were administered intranasally beginning at arthritis onset. Clinical and histologic scores were assessed, and cytokine levels were determined in the serum or in supernatants from splenocytes. Characteristics of T cell subsets in response to different peptides were analyzed both in vivo and in vitro. RESULTS: Intranasal administration of wild CII263-272 peptide, wild HA308-317 peptide, or altered HA308-317 peptide could significantly ameliorate CIA, but altered HA308-317 peptide showed greater therapeutic effects than wild CII263-272 peptide and wild HA308-317 peptide. The effect of altered HA308-317 peptide was associated with a substantial decrease in production of interleukin-17 (IL-17) and interferon-gamma (IFNgamma) and with a marked increase in production of IL-10 and transforming growth factor beta, both in serum and in supernatants from splenocytes treated with altered HA308-317 peptide. Both the number and function of CD4+ Treg cells were significantly up-regulated by altered HA308-317 peptide, with a decreased induction of Th1 cells (CD4+IFNgamma+) and Th17 cells (CD4+IL-17+). Adoptive transfer of CD4+CD25+ T cells from altered HA308-317 peptide-treated mice resulted in greater suppressive capacity in ameliorating CIA severity than did adoptive transfer of CD4+CD25+ T cells from wild HA308-317 peptide-treated, wild CII263-272 peptide-treated, or irrelevant peptide-treated mice. CONCLUSION: Intranasal administration of altered HA308-317 peptide potently suppressed the severity of CIA by increasing the number and function of CD4+ Treg cells, suggesting that altered HA308-317 peptide might be a promising candidate for treatment of rheumatoid arthritis. © 2012, American College of Rheumatology.
Persistent Identifierhttp://hdl.handle.net/10722/164841
ISSN
2013 Impact Factor: 7.871
ISI Accession Number ID

 

Author Affiliations
  1. The University of Hong Kong
  2. Imperial College London
  3. Peking University
DC FieldValueLanguage
dc.contributor.authorSun, Jen_US
dc.contributor.authorLi, Ren_US
dc.contributor.authorGuo, Jen_US
dc.contributor.authorJia, Yen_US
dc.contributor.authorSun, Xen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorLi, Yen_US
dc.contributor.authorHuang, Fen_US
dc.contributor.authorLu, Len_US
dc.contributor.authorLi, Zen_US
dc.date.accessioned2012-09-20T08:10:45Z-
dc.date.available2012-09-20T08:10:45Z-
dc.date.issued2012en_US
dc.identifier.citationArthritis & Rheumatism, 2012, v. 64 n. 7, p. 2158-2168en_US
dc.identifier.issn0004-3591en_US
dc.identifier.urihttp://hdl.handle.net/10722/164841-
dc.description.abstractOBJECTIVE: To investigate the inhibitory effect and possible mechanism of a novel influenza virus hemagglutinin 308-317 peptide (altered HA308-317 peptide) in collagen-induced arthritis (CIA). METHODS: CIA was induced in DBA/1 mice by immunization with type II collagen (CII). Altered HA308-317 peptide, wild HA308-317 peptide, wild CII263-272 peptide, and irrelevant peptide were administered intranasally beginning at arthritis onset. Clinical and histologic scores were assessed, and cytokine levels were determined in the serum or in supernatants from splenocytes. Characteristics of T cell subsets in response to different peptides were analyzed both in vivo and in vitro. RESULTS: Intranasal administration of wild CII263-272 peptide, wild HA308-317 peptide, or altered HA308-317 peptide could significantly ameliorate CIA, but altered HA308-317 peptide showed greater therapeutic effects than wild CII263-272 peptide and wild HA308-317 peptide. The effect of altered HA308-317 peptide was associated with a substantial decrease in production of interleukin-17 (IL-17) and interferon-gamma (IFNgamma) and with a marked increase in production of IL-10 and transforming growth factor beta, both in serum and in supernatants from splenocytes treated with altered HA308-317 peptide. Both the number and function of CD4+ Treg cells were significantly up-regulated by altered HA308-317 peptide, with a decreased induction of Th1 cells (CD4+IFNgamma+) and Th17 cells (CD4+IL-17+). Adoptive transfer of CD4+CD25+ T cells from altered HA308-317 peptide-treated mice resulted in greater suppressive capacity in ameliorating CIA severity than did adoptive transfer of CD4+CD25+ T cells from wild HA308-317 peptide-treated, wild CII263-272 peptide-treated, or irrelevant peptide-treated mice. CONCLUSION: Intranasal administration of altered HA308-317 peptide potently suppressed the severity of CIA by increasing the number and function of CD4+ Treg cells, suggesting that altered HA308-317 peptide might be a promising candidate for treatment of rheumatoid arthritis. © 2012, American College of Rheumatology.-
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.interscience.wiley.com/jpages/0004-3591/-
dc.relation.ispartofArthritis & Rheumatismen_US
dc.rightsArthritis & Rheumatism. Copyright © John Wiley & Sons, Inc.-
dc.subjectCollagen type 2-
dc.subjectGamma interferon-
dc.subjectInfluenza virus hemagglutinin[308-317]-
dc.subjectInterleukin 10-
dc.subjectInterleukin 6-
dc.subject.meshArthritis, Experimental - drug therapy - immunology - pathology-
dc.subject.meshCell proliferation - drug effects-
dc.subject.meshHemagglutinins, Viral - pharmacology - therapeutic use-
dc.subject.meshInterleukin-10 - metabolism-
dc.subject.meshT-Lymphocytes, Regulatory - drug effects - immunology - pathology-
dc.subject.meshInterferon-gamma - metabolism-
dc.subject.meshInterleukin-17 - metabolism-
dc.subject.meshTransforming growth factor beta - metabolism-
dc.titleSuperior molecularly altered influenza virus hemagglutinin peptide 308-317 inhibits collagen-induced arthritis by inducing CD4+ treg cell expansionen_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1529-0131 (Electronic) 0004-3591 (Linkin&volume=64&issue=7&spage=2158&epage=68&date=2012&atitle=Superior+molecularly+altered+influenza+virus+hemagglutinin+peptide+308-317+inhibits+collagen-induced+arthritis+by+inducing+CD4++Treg+cell+expansionen_US
dc.identifier.emailLu, L: liweilu@hkucc.hku.hken_US
dc.identifier.emailLi, Z: zgli@yahoo.cn-
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1002/art.34372-
dc.identifier.pmid22231228-
dc.identifier.scopuseid_2-s2.0-84863197516-
dc.identifier.hkuros208156en_US
dc.identifier.volume64en_US
dc.identifier.issue7en_US
dc.identifier.spage2158en_US
dc.identifier.epage2168en_US
dc.identifier.isiWOS:000305742800012-
dc.publisher.placeUnited States-

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