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Article: Quantification of BK viral load in asymptomatic renal allograft recipients

TitleQuantification of BK viral load in asymptomatic renal allograft recipients
Authors
KeywordsImmunosuppression
Nephropathy
Polyoma BK virus
Quantitative PCR
Renal transplantation
Issue Date2012
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/0886022x.asp
Citation
Renal Failure, 2012, v. 34 n. 5, p. 550-554 How to Cite?
Abstract
Introduction: Polyoma BK virus (BKV) has recently been identified to cause renal allograft dysfunction, which manifests as polyomavirus-associated nephropathy (PVAN). However, the presence and level of BKV DNA in renal allograft patients with good and stable renal function have remained undetermined. Methods: In this prospective study, serum samples were collected from a total of 45 renal allograft recipients with serum creatinine <155 μmol/L. In 17 patients, whose duration of transplantation was under 2 years, samples were collected at 34-month intervals for up to 2 years after transplantation. BK viral load was quantified using quantitative polymerase chain reaction (Q-PCR). Results: The BK viral load in asymptomatic renal allograft recipients was independent of the duration of transplantation and did not correlate with allograft function. The mean (± SD) level of viremia was 552.80 ± 1931.00 genome copies/mL, with 92.9 of patients having low levels of viremia corresponding to <1 × 10 3 copies/mL. In contrast, patients with proven PVAN had levels in the range of 10 6 copies/mL. Conclusions: The prevailing BK viral load in asymptomatic renal allograft patients is quantifiably low. Our findings may guide optimal immunosuppressive modulation in PVAN cases, where judicious manipulation of immunosuppression is required without inciting allograft rejection. Copyright © Informa Healthcare USA, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/163488
ISSN
2013 Impact Factor: 0.775
ISI Accession Number ID
References

 

Author Affiliations
  1. The University of Hong Kong
DC FieldValueLanguage
dc.contributor.authorChan, GCWen_HK
dc.contributor.authorLeung, AYHen_HK
dc.contributor.authorWong, ASYen_HK
dc.contributor.authorChan, KWen_HK
dc.contributor.authorKwong, YLen_HK
dc.contributor.authorLai, KNen_HK
dc.contributor.authorTang, SCWen_HK
dc.date.accessioned2012-09-05T05:32:05Z-
dc.date.available2012-09-05T05:32:05Z-
dc.date.issued2012en_HK
dc.identifier.citationRenal Failure, 2012, v. 34 n. 5, p. 550-554en_HK
dc.identifier.issn0886-022Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/163488-
dc.description.abstractIntroduction: Polyoma BK virus (BKV) has recently been identified to cause renal allograft dysfunction, which manifests as polyomavirus-associated nephropathy (PVAN). However, the presence and level of BKV DNA in renal allograft patients with good and stable renal function have remained undetermined. Methods: In this prospective study, serum samples were collected from a total of 45 renal allograft recipients with serum creatinine <155 μmol/L. In 17 patients, whose duration of transplantation was under 2 years, samples were collected at 34-month intervals for up to 2 years after transplantation. BK viral load was quantified using quantitative polymerase chain reaction (Q-PCR). Results: The BK viral load in asymptomatic renal allograft recipients was independent of the duration of transplantation and did not correlate with allograft function. The mean (± SD) level of viremia was 552.80 ± 1931.00 genome copies/mL, with 92.9 of patients having low levels of viremia corresponding to <1 × 10 3 copies/mL. In contrast, patients with proven PVAN had levels in the range of 10 6 copies/mL. Conclusions: The prevailing BK viral load in asymptomatic renal allograft patients is quantifiably low. Our findings may guide optimal immunosuppressive modulation in PVAN cases, where judicious manipulation of immunosuppression is required without inciting allograft rejection. Copyright © Informa Healthcare USA, Inc.en_HK
dc.languageengen_US
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/0886022x.aspen_HK
dc.relation.ispartofRenal Failureen_HK
dc.rightsRenal Failure. Copyright © Informa Healthcare.-
dc.subjectImmunosuppressionen_HK
dc.subjectNephropathyen_HK
dc.subjectPolyoma BK virusen_HK
dc.subjectQuantitative PCRen_HK
dc.subjectRenal transplantationen_HK
dc.subject.meshBK Virus - genetics - isolation and purification-
dc.subject.meshDNA, Viral - analysis-
dc.subject.meshGraft Rejection - virology-
dc.subject.meshKidney Diseases - virology-
dc.subject.meshKidney Transplantation-
dc.titleQuantification of BK viral load in asymptomatic renal allograft recipientsen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, KW: hrmtckw@hku.hken_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailTang, SCW: scwtang@hku.hken_HK
dc.identifier.authorityChan, KW=rp00330en_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityTang, SCW=rp00480en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.3109/0886022X.2012.664808en_HK
dc.identifier.pmid22390257en_HK
dc.identifier.scopuseid_2-s2.0-84860786016en_HK
dc.identifier.hkuros203970-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84860786016&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume34en_HK
dc.identifier.issue5en_HK
dc.identifier.spage550en_HK
dc.identifier.epage554en_HK
dc.identifier.isiWOS:000303834600002-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChan, GCW=55211294300en_HK
dc.identifier.scopusauthoridLeung, AYH=55091367300en_HK
dc.identifier.scopusauthoridWong, ASY=7403144356en_HK
dc.identifier.scopusauthoridChan, KW=16444133100en_HK
dc.identifier.scopusauthoridKwong, YL=55158018200en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridTang, SCW=7403437082en_HK

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