Article: Quantification of BK viral load in asymptomatic renal allograft recipients

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TitleQuantification of BK viral load in asymptomatic renal allograft recipients
AuthorsChan, GCW1
Leung, AYH1
Wong, ASY1
Chan, KW1
Kwong, YL1
Lai, KN1
Tang, SCW1
KeywordsImmunosuppression
Nephropathy
Polyoma BK virus
Quantitative PCR
Renal transplantation
Issue Date2012
PublisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/0886022x.asp
CitationRenal Failure, 2012, v. 34 n. 5, p. 550-554 [How to Cite?]
DOI: http://dx.doi.org/10.3109/0886022X.2012.664808
AbstractIntroduction: Polyoma BK virus (BKV) has recently been identified to cause renal allograft dysfunction, which manifests as polyomavirus-associated nephropathy (PVAN). However, the presence and level of BKV DNA in renal allograft patients with good and stable renal function have remained undetermined. Methods: In this prospective study, serum samples were collected from a total of 45 renal allograft recipients with serum creatinine <155 μmol/L. In 17 patients, whose duration of transplantation was under 2 years, samples were collected at 34-month intervals for up to 2 years after transplantation. BK viral load was quantified using quantitative polymerase chain reaction (Q-PCR). Results: The BK viral load in asymptomatic renal allograft recipients was independent of the duration of transplantation and did not correlate with allograft function. The mean (± SD) level of viremia was 552.80 ± 1931.00 genome copies/mL, with 92.9 of patients having low levels of viremia corresponding to <1 × 10 3 copies/mL. In contrast, patients with proven PVAN had levels in the range of 10 6 copies/mL. Conclusions: The prevailing BK viral load in asymptomatic renal allograft patients is quantifiably low. Our findings may guide optimal immunosuppressive modulation in PVAN cases, where judicious manipulation of immunosuppression is required without inciting allograft rejection. Copyright © Informa Healthcare USA, Inc.
ISSN0886-022X
2011 Impact Factor: 0.824
2011 SCImago Journal Rankings: 0.082
DOIhttp://dx.doi.org/10.3109/0886022X.2012.664808
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorChan, GCW
dc.contributor.authorLeung, AYH
dc.contributor.authorWong, ASY
dc.contributor.authorChan, KW
dc.contributor.authorKwong, YL
dc.contributor.authorLai, KN
dc.contributor.authorTang, SCW
dc.date.accessioned2012-09-05T05:32:05Z
dc.date.available2012-09-05T05:32:05Z
dc.date.issued2012
dc.description.abstractIntroduction: Polyoma BK virus (BKV) has recently been identified to cause renal allograft dysfunction, which manifests as polyomavirus-associated nephropathy (PVAN). However, the presence and level of BKV DNA in renal allograft patients with good and stable renal function have remained undetermined. Methods: In this prospective study, serum samples were collected from a total of 45 renal allograft recipients with serum creatinine <155 μmol/L. In 17 patients, whose duration of transplantation was under 2 years, samples were collected at 34-month intervals for up to 2 years after transplantation. BK viral load was quantified using quantitative polymerase chain reaction (Q-PCR). Results: The BK viral load in asymptomatic renal allograft recipients was independent of the duration of transplantation and did not correlate with allograft function. The mean (± SD) level of viremia was 552.80 ± 1931.00 genome copies/mL, with 92.9 of patients having low levels of viremia corresponding to <1 × 10 3 copies/mL. In contrast, patients with proven PVAN had levels in the range of 10 6 copies/mL. Conclusions: The prevailing BK viral load in asymptomatic renal allograft patients is quantifiably low. Our findings may guide optimal immunosuppressive modulation in PVAN cases, where judicious manipulation of immunosuppression is required without inciting allograft rejection. Copyright © Informa Healthcare USA, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationRenal Failure, 2012, v. 34 n. 5, p. 550-554 [How to Cite?]
DOI: http://dx.doi.org/10.3109/0886022X.2012.664808
dc.identifier.doihttp://dx.doi.org/10.3109/0886022X.2012.664808
dc.identifier.epage554
dc.identifier.hkuros203970
dc.identifier.issn0886-022X
2011 Impact Factor: 0.824
2011 SCImago Journal Rankings: 0.082
dc.identifier.issue5
dc.identifier.pmid22390257
dc.identifier.scopuseid_2-s2.0-84860786016
dc.identifier.spage550
dc.identifier.urihttp://hdl.handle.net/10722/163488
dc.identifier.volume34
dc.languageeng
dc.publisherInforma Healthcare. The Journal's web site is located at http://www.tandf.co.uk/journals/titles/0886022x.asp
dc.publisher.placeUnited States
dc.relation.ispartofRenal Failure
dc.relation.referencesReferences in Scopus
dc.rightsRenal Failure. Copyright © Informa Healthcare.
dc.subject.meshBK Virus - genetics - isolation and purification
dc.subject.meshDNA, Viral - analysis
dc.subject.meshGraft Rejection - virology
dc.subject.meshKidney Diseases - virology
dc.subject.meshKidney Transplantation
dc.subjectImmunosuppression
dc.subjectNephropathy
dc.subjectPolyoma BK virus
dc.subjectQuantitative PCR
dc.subjectRenal transplantation
dc.titleQuantification of BK viral load in asymptomatic renal allograft recipients
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong