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Article: In vitro enhanced chemotaxis of CD25+ mononuclear cells in patients with familial IgAN through glomerulotubular interactions

TitleIn vitro enhanced chemotaxis of CD25+ mononuclear cells in patients with familial IgAN through glomerulotubular interactions
Authors
KeywordsCD25
Glomerulotubular interaction
IgA nephropathy
Multiplex IgAN family
Issue Date2010
Citation
American Journal Of Physiology - Renal Physiology, 2010, v. 299 n. 2, p. F359-F368 How to Cite?
AbstractTubulointerstitial infiltration of immunocompetent cells is often associated with a more rapid progression in IgA nephropathy (IgAN). Using an in vitro Transwell coculture system, we examined the chemotactic response of peripheral blood mononuclear cells to proximal tubular epithelial cells (PTEC) following activation by conditioned medium prepared from mesangial cells cultured with macromolecular IgA1 from 60 patients with multiplex familial IgAN (MpIgAN) and 91 of their asymptomatic relatives; 43 patients with sporadic IgAN (SpIgAN) and 90 of their asymptomatic relatives; and 43 healthy subjects. Compared with SpIgAN patients, PTEC activated by conditioned medium from patients with MpIgAN had elevated gene expression of a spectrum of C-C, C-X-C chemokines and proinflammatory cytokines, with prominent expressions of interleukin-6, interleukin-8, and tumor necrosis factor-α. In response to conditioned medium from patients with familial IgAN, there was a significant increase in chemotaxis of CD45+ cells, CD3+, CD4+, CD8+, CD20+ lymphocytes, and monocytes with CD25 expression. Our findings suggest that compared with SpIgAN patients, macromolecular IgA1 taken from MpIgAN patients is more pathogenic to cultured PTEC through a glomerulotubular interaction. A long-term follow-up is needed to better define the prognostic course for familial IgAN and to clarify the risk of developing IgAN in initially asymptomatic relatives from a multiplex IgAN family. Copyright © 2010 the American Physiological Society.
Persistent Identifierhttp://hdl.handle.net/10722/163325
ISSN
2008 Impact Factor: 3.89
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CommitteeHKU 7697/07M
7669/08M
L & T Charitable Foundation
House of INDOCAFE
Funding Information:

The study was supported by the Hong Kong Research Grant Committee (HKU 7697/07M and 7669/08M), and L. Y. Y. Chan was partly supported by the L & T Charitable Foundation and the House of INDOCAFE.

References

 

DC FieldValueLanguage
dc.contributor.authorTam, KYen_US
dc.contributor.authorLeung, JCKen_US
dc.contributor.authorChan, LYYen_US
dc.contributor.authorLam, MFen_US
dc.contributor.authorTang, SCWen_US
dc.contributor.authorLai, KNen_US
dc.date.accessioned2012-09-05T05:30:04Z-
dc.date.available2012-09-05T05:30:04Z-
dc.date.issued2010en_US
dc.identifier.citationAmerican Journal Of Physiology - Renal Physiology, 2010, v. 299 n. 2, p. F359-F368en_US
dc.identifier.issn0363-6127en_US
dc.identifier.urihttp://hdl.handle.net/10722/163325-
dc.description.abstractTubulointerstitial infiltration of immunocompetent cells is often associated with a more rapid progression in IgA nephropathy (IgAN). Using an in vitro Transwell coculture system, we examined the chemotactic response of peripheral blood mononuclear cells to proximal tubular epithelial cells (PTEC) following activation by conditioned medium prepared from mesangial cells cultured with macromolecular IgA1 from 60 patients with multiplex familial IgAN (MpIgAN) and 91 of their asymptomatic relatives; 43 patients with sporadic IgAN (SpIgAN) and 90 of their asymptomatic relatives; and 43 healthy subjects. Compared with SpIgAN patients, PTEC activated by conditioned medium from patients with MpIgAN had elevated gene expression of a spectrum of C-C, C-X-C chemokines and proinflammatory cytokines, with prominent expressions of interleukin-6, interleukin-8, and tumor necrosis factor-α. In response to conditioned medium from patients with familial IgAN, there was a significant increase in chemotaxis of CD45+ cells, CD3+, CD4+, CD8+, CD20+ lymphocytes, and monocytes with CD25 expression. Our findings suggest that compared with SpIgAN patients, macromolecular IgA1 taken from MpIgAN patients is more pathogenic to cultured PTEC through a glomerulotubular interaction. A long-term follow-up is needed to better define the prognostic course for familial IgAN and to clarify the risk of developing IgAN in initially asymptomatic relatives from a multiplex IgAN family. Copyright © 2010 the American Physiological Society.en_US
dc.languageengen_US
dc.relation.ispartofAmerican Journal of Physiology - Renal Physiologyen_US
dc.subjectCD25-
dc.subjectGlomerulotubular interaction-
dc.subjectIgA nephropathy-
dc.subjectMultiplex IgAN family-
dc.subject.meshCase-Control Studiesen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshChemotaxis, Leukocyteen_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshCulture Media, Conditioned - Metabolismen_US
dc.subject.meshCytokines - Genetics - Metabolismen_US
dc.subject.meshEpithelial Cells - Immunology - Pathologyen_US
dc.subject.meshGene Expression Regulationen_US
dc.subject.meshGenetic Predisposition To Diseaseen_US
dc.subject.meshGlomerulonephritis, Iga - Genetics - Immunology - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoglobulin A - Metabolismen_US
dc.subject.meshInflammation Mediators - Metabolismen_US
dc.subject.meshInterleukin-2 Receptor Alpha Subunit - Analysisen_US
dc.subject.meshKidney Tubules, Proximal - Immunology - Pathologyen_US
dc.subject.meshLeukocytes, Mononuclear - Immunologyen_US
dc.subject.meshMesangial Cells - Immunology - Pathologyen_US
dc.subject.meshPedigreeen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshTime Factorsen_US
dc.titleIn vitro enhanced chemotaxis of CD25+ mononuclear cells in patients with familial IgAN through glomerulotubular interactionsen_US
dc.typeArticleen_US
dc.identifier.emailLeung, JCK:jckleung@hku.hken_US
dc.identifier.emailTang, SCW:scwtang@hku.hken_US
dc.identifier.emailLai, KN:knlai@hku.hken_US
dc.identifier.authorityLeung, JCK=rp00448en_US
dc.identifier.authorityTang, SCW=rp00480en_US
dc.identifier.authorityLai, KN=rp00324en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1152/ajprenal.00664.2009en_US
dc.identifier.pmid20484297-
dc.identifier.scopuseid_2-s2.0-77955366397en_US
dc.identifier.hkuros173993-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955366397&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume299en_US
dc.identifier.issue2en_US
dc.identifier.spageF359en_US
dc.identifier.epageF368en_US
dc.identifier.isiWOS:000280566600009-
dc.identifier.scopusauthoridTam, KY=25930206700en_US
dc.identifier.scopusauthoridLeung, JCK=7202180349en_US
dc.identifier.scopusauthoridChan, LYY=35336076700en_US
dc.identifier.scopusauthoridLam, MF=7202630163en_US
dc.identifier.scopusauthoridTang, SCW=7403437082en_US
dc.identifier.scopusauthoridLai, KN=7402135706en_US

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