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Article: Quantitation of covalently closed circular hepatitis B virus DNA in chronic B patients
Title | Quantitation of covalently closed circular hepatitis B virus DNA in chronic B patients |
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Authors | |
Issue Date | 2004 |
Publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/ |
Citation | Hepatology, 2004, v. 40 n. 3, p. 727-737 How to Cite? |
Abstract | This study examined a signal amplification assay, the Invader assay, for the quantitation of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in liver biopsies and sera. DNA was extracted from liver biopsy and serum samples were collected from 16 hepatitis B e antigen (HBeAg)-positive and 36 antibody-to-HBeAg-positive (anti-HBe-positive) chronic hepatitis B patients. The amount of total HBV DNA and cccDNA was measured using the Invader assay. Anti-HBe-positive patients had lower median total intrahepatic HBV DNA (P < .001) and intrahepatic cccDNA levels (P = .001) than HBeAg-positive patients. Intrahepatic cccDNA correlated positively with the total intrahepatic HBV DNA (r = 0.950, P < .001). However, the proportion of intrahepatic HBV DNA in the form of cccDNA was inversely related to the amount of total intrahepatic HBV DNA (r = -0.822, P < .001). A small amount of cccDNA was detected in 39 of 52 (75%) serum samples. Anti-HBe-positive patients had lower median serum cccDNA levels than HBeAg-positive patients (P = .002). Serum HBV DNA correlated positively with intrahepatic total HBV DNA (r = 0.778, P < .001) and intrahepatic cccDNA (r = 0.481, P = .002). In conclusion, the Invader assay is a reliable assay for the quantitation of cccDNA. Serum and intrahepatic total HBV DNA and cccDNA levels become lower as the disease progresses from HBeAg-positive to anti-HBe-positive phase, with cccDNA becoming the predominant form of intrahepatic HBV DNA. |
Persistent Identifier | http://hdl.handle.net/10722/163181 |
ISSN | 2021 Impact Factor: 17.298 2020 SCImago Journal Rankings: 5.488 |
ISI Accession Number ID | |
References |
DC Field | Value | Language |
---|---|---|
dc.contributor.author | Wong, DKH | en_US |
dc.contributor.author | Yuen, MF | en_US |
dc.contributor.author | Yuan, H | en_US |
dc.contributor.author | Sum, SSM | en_US |
dc.contributor.author | Hui, GK | en_US |
dc.contributor.author | Hall, J | en_US |
dc.contributor.author | Lai, CL | en_US |
dc.date.accessioned | 2012-09-05T05:28:28Z | - |
dc.date.available | 2012-09-05T05:28:28Z | - |
dc.date.issued | 2004 | en_US |
dc.identifier.citation | Hepatology, 2004, v. 40 n. 3, p. 727-737 | en_US |
dc.identifier.issn | 0270-9139 | en_US |
dc.identifier.uri | http://hdl.handle.net/10722/163181 | - |
dc.description.abstract | This study examined a signal amplification assay, the Invader assay, for the quantitation of hepatitis B virus (HBV) covalently closed circular DNA (cccDNA) in liver biopsies and sera. DNA was extracted from liver biopsy and serum samples were collected from 16 hepatitis B e antigen (HBeAg)-positive and 36 antibody-to-HBeAg-positive (anti-HBe-positive) chronic hepatitis B patients. The amount of total HBV DNA and cccDNA was measured using the Invader assay. Anti-HBe-positive patients had lower median total intrahepatic HBV DNA (P < .001) and intrahepatic cccDNA levels (P = .001) than HBeAg-positive patients. Intrahepatic cccDNA correlated positively with the total intrahepatic HBV DNA (r = 0.950, P < .001). However, the proportion of intrahepatic HBV DNA in the form of cccDNA was inversely related to the amount of total intrahepatic HBV DNA (r = -0.822, P < .001). A small amount of cccDNA was detected in 39 of 52 (75%) serum samples. Anti-HBe-positive patients had lower median serum cccDNA levels than HBeAg-positive patients (P = .002). Serum HBV DNA correlated positively with intrahepatic total HBV DNA (r = 0.778, P < .001) and intrahepatic cccDNA (r = 0.481, P = .002). In conclusion, the Invader assay is a reliable assay for the quantitation of cccDNA. Serum and intrahepatic total HBV DNA and cccDNA levels become lower as the disease progresses from HBeAg-positive to anti-HBe-positive phase, with cccDNA becoming the predominant form of intrahepatic HBV DNA. | en_US |
dc.language | eng | en_US |
dc.publisher | John Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/ | en_US |
dc.relation.ispartof | Hepatology | en_US |
dc.rights | Hepatology. Copyright © John Wiley & Sons, Inc. | - |
dc.title | Quantitation of covalently closed circular hepatitis B virus DNA in chronic B patients | en_US |
dc.type | Article | en_US |
dc.identifier.email | Wong, DKH:danywong@hku.hk | en_US |
dc.identifier.email | Yuen, MF:mfyuen@hkucc.hku.hk | en_US |
dc.identifier.email | Lai, CL:hrmelcl@hku.hk | en_US |
dc.identifier.authority | Wong, DKH=rp00492 | en_US |
dc.identifier.authority | Yuen, MF=rp00479 | en_US |
dc.identifier.authority | Lai, CL=rp00314 | en_US |
dc.description.nature | link_to_OA_fulltext | en_US |
dc.identifier.doi | 10.1002/hep.20353 | en_US |
dc.identifier.pmid | 15349913 | - |
dc.identifier.scopus | eid_2-s2.0-4644296081 | en_US |
dc.identifier.hkuros | 97685 | - |
dc.relation.references | http://www.scopus.com/mlt/select.url?eid=2-s2.0-4644296081&selection=ref&src=s&origin=recordpage | en_US |
dc.identifier.volume | 40 | en_US |
dc.identifier.issue | 3 | en_US |
dc.identifier.spage | 727 | en_US |
dc.identifier.epage | 737 | en_US |
dc.identifier.isi | WOS:000223684500030 | - |
dc.publisher.place | United States | en_US |
dc.identifier.scopusauthorid | Wong, DKH=7401535819 | en_US |
dc.identifier.scopusauthorid | Yuen, MF=7102031955 | en_US |
dc.identifier.scopusauthorid | Yuan, H=7402446707 | en_US |
dc.identifier.scopusauthorid | Sum, SSM=6603889128 | en_US |
dc.identifier.scopusauthorid | Hui, GK=7005017737 | en_US |
dc.identifier.scopusauthorid | Hall, J=7407379623 | en_US |
dc.identifier.scopusauthorid | Lai, CL=7403086396 | en_US |
dc.identifier.issnl | 0270-9139 | - |