Article: Peritoneal mesothelial cell culture and biology
| Title | Peritoneal mesothelial cell culture and biology |
|---|---|
| Authors | Yung, S1 Li, FK1 Chan, TM1 |
| Issue Date | 2006 |
| Publisher | Multimed, Inc. The Journal's web site is located at http://pdiconnect.com |
| Citation | Peritoneal Dialysis International, 2006, v. 26 n. 2, p. 162-173 [How to Cite?] |
| Abstract | The peritoneal mesothelium is composed of an extensive monolayer of mesothelial cells that lines the body's serous cavity and internal organs and was previously thought to act principally as a protective nonadhesive lubricating surface to facilitate intracoelomic movement. With the introduction of peritoneal dialysis over three decades ago, there has been much interest in the cell biology of peritoneal mesothelial cells. Independent studies have highlighted specific properties of the peritoneal mesothelial cell, including antigen presentation, regenerative properties, clearance of fibrin; synthesis of cytokines, growth factors, and matrix proteins; and secretion of lubricants to protect the tissue from abrasion, adhesion, infection, and tumor dissemination. It is now evident that the mesothelium is not merely a passive membrane but, rather, a dynamic membrane that contributes substantially to the structural, functional, and homeostatic properties of the pentoneum. Since peritoneal mesothelial cells in culture possess immunohistochemical markers identical to mesothelial stem cells, the culture of mesothelial cells offers researchers an essential tool to assess their morphologic, structural, and functional properties. This review will discuss current procedures to isolate peritoneal mesothelial cells from human omental specimens, animal sources, and spent dialysate. Furthermore, the functional and morphologic properties of mesothelial cells are discussed, together with the potential use of mesothelial cell culture in research and clinical applications. Copyright © 2006 International Society for Peritoneal Dialysis. |
| ISSN | 0896-8608 2011 Impact Factor: 2.097 2011 SCImago Journal Rankings: 0.091 |
| References | References in Scopus |
| dc.contributor.author | Yung, S |
|---|---|
| dc.contributor.author | Li, FK |
| dc.contributor.author | Chan, TM |
| dc.date.accessioned | 2012-09-05T05:26:30Z |
| dc.date.available | 2012-09-05T05:26:30Z |
| dc.date.issued | 2006 |
| dc.description.abstract | The peritoneal mesothelium is composed of an extensive monolayer of mesothelial cells that lines the body's serous cavity and internal organs and was previously thought to act principally as a protective nonadhesive lubricating surface to facilitate intracoelomic movement. With the introduction of peritoneal dialysis over three decades ago, there has been much interest in the cell biology of peritoneal mesothelial cells. Independent studies have highlighted specific properties of the peritoneal mesothelial cell, including antigen presentation, regenerative properties, clearance of fibrin; synthesis of cytokines, growth factors, and matrix proteins; and secretion of lubricants to protect the tissue from abrasion, adhesion, infection, and tumor dissemination. It is now evident that the mesothelium is not merely a passive membrane but, rather, a dynamic membrane that contributes substantially to the structural, functional, and homeostatic properties of the pentoneum. Since peritoneal mesothelial cells in culture possess immunohistochemical markers identical to mesothelial stem cells, the culture of mesothelial cells offers researchers an essential tool to assess their morphologic, structural, and functional properties. This review will discuss current procedures to isolate peritoneal mesothelial cells from human omental specimens, animal sources, and spent dialysate. Furthermore, the functional and morphologic properties of mesothelial cells are discussed, together with the potential use of mesothelial cell culture in research and clinical applications. Copyright © 2006 International Society for Peritoneal Dialysis. |
| dc.description.nature | Link_to_subscribed_fulltext |
| dc.identifier.citation | Peritoneal Dialysis International, 2006, v. 26 n. 2, p. 162-173 [How to Cite?] |
| dc.identifier.epage | 173 |
| dc.identifier.hkuros | 117899 |
| dc.identifier.issn | 0896-8608 2011 Impact Factor: 2.097 2011 SCImago Journal Rankings: 0.091 |
| dc.identifier.issue | 2 |
| dc.identifier.pmid | 16623418 |
| dc.identifier.scopus | eid_2-s2.0-33747135979 |
| dc.identifier.spage | 162 |
| dc.identifier.uri | http://hdl.handle.net/10722/163010 |
| dc.identifier.volume | 26 |
| dc.language | eng |
| dc.publisher | Multimed, Inc. The Journal's web site is located at http://pdiconnect.com |
| dc.publisher.place | Canada |
| dc.relation.ispartof | Peritoneal Dialysis International |
| dc.relation.references | References in Scopus |
| dc.subject.mesh | Cell Culture Techniques - Methods |
| dc.subject.mesh | Cells, Cultured |
| dc.subject.mesh | Epithelial Cells - Physiology |
| dc.subject.mesh | Humans |
| dc.subject.mesh | Peritoneum - Cytology |
| dc.title | Peritoneal mesothelial cell culture and biology |
| dc.type | Article |
Author Affiliations
- The University of Hong Kong