File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Peritoneal mesothelial cell culture and biology
  • Basic View
  • Metadata View
  • XML View
TitlePeritoneal mesothelial cell culture and biology
 
AuthorsYung, S1
Li, FK1
Chan, TM1
 
Issue Date2006
 
PublisherMultimed, Inc. The Journal's web site is located at http://pdiconnect.com
 
CitationPeritoneal Dialysis International, 2006, v. 26 n. 2, p. 162-173 [How to Cite?]
 
AbstractThe peritoneal mesothelium is composed of an extensive monolayer of mesothelial cells that lines the body's serous cavity and internal organs and was previously thought to act principally as a protective nonadhesive lubricating surface to facilitate intracoelomic movement. With the introduction of peritoneal dialysis over three decades ago, there has been much interest in the cell biology of peritoneal mesothelial cells. Independent studies have highlighted specific properties of the peritoneal mesothelial cell, including antigen presentation, regenerative properties, clearance of fibrin; synthesis of cytokines, growth factors, and matrix proteins; and secretion of lubricants to protect the tissue from abrasion, adhesion, infection, and tumor dissemination. It is now evident that the mesothelium is not merely a passive membrane but, rather, a dynamic membrane that contributes substantially to the structural, functional, and homeostatic properties of the pentoneum. Since peritoneal mesothelial cells in culture possess immunohistochemical markers identical to mesothelial stem cells, the culture of mesothelial cells offers researchers an essential tool to assess their morphologic, structural, and functional properties. This review will discuss current procedures to isolate peritoneal mesothelial cells from human omental specimens, animal sources, and spent dialysate. Furthermore, the functional and morphologic properties of mesothelial cells are discussed, together with the potential use of mesothelial cell culture in research and clinical applications. Copyright © 2006 International Society for Peritoneal Dialysis.
 
ISSN0896-8608
2013 Impact Factor: 2.199
2013 SCImago Journal Rankings: 1.189
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorYung, S
 
dc.contributor.authorLi, FK
 
dc.contributor.authorChan, TM
 
dc.date.accessioned2012-09-05T05:26:30Z
 
dc.date.available2012-09-05T05:26:30Z
 
dc.date.issued2006
 
dc.description.abstractThe peritoneal mesothelium is composed of an extensive monolayer of mesothelial cells that lines the body's serous cavity and internal organs and was previously thought to act principally as a protective nonadhesive lubricating surface to facilitate intracoelomic movement. With the introduction of peritoneal dialysis over three decades ago, there has been much interest in the cell biology of peritoneal mesothelial cells. Independent studies have highlighted specific properties of the peritoneal mesothelial cell, including antigen presentation, regenerative properties, clearance of fibrin; synthesis of cytokines, growth factors, and matrix proteins; and secretion of lubricants to protect the tissue from abrasion, adhesion, infection, and tumor dissemination. It is now evident that the mesothelium is not merely a passive membrane but, rather, a dynamic membrane that contributes substantially to the structural, functional, and homeostatic properties of the pentoneum. Since peritoneal mesothelial cells in culture possess immunohistochemical markers identical to mesothelial stem cells, the culture of mesothelial cells offers researchers an essential tool to assess their morphologic, structural, and functional properties. This review will discuss current procedures to isolate peritoneal mesothelial cells from human omental specimens, animal sources, and spent dialysate. Furthermore, the functional and morphologic properties of mesothelial cells are discussed, together with the potential use of mesothelial cell culture in research and clinical applications. Copyright © 2006 International Society for Peritoneal Dialysis.
 
dc.description.natureLink_to_subscribed_fulltext
 
dc.identifier.citationPeritoneal Dialysis International, 2006, v. 26 n. 2, p. 162-173 [How to Cite?]
 
dc.identifier.epage173
 
dc.identifier.hkuros117899
 
dc.identifier.issn0896-8608
2013 Impact Factor: 2.199
2013 SCImago Journal Rankings: 1.189
 
dc.identifier.issue2
 
dc.identifier.pmid16623418
 
dc.identifier.scopuseid_2-s2.0-33747135979
 
dc.identifier.spage162
 
dc.identifier.urihttp://hdl.handle.net/10722/163010
 
dc.identifier.volume26
 
dc.languageeng
 
dc.publisherMultimed, Inc. The Journal's web site is located at http://pdiconnect.com
 
dc.publisher.placeCanada
 
dc.relation.ispartofPeritoneal Dialysis International
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshCell Culture Techniques - Methods
 
dc.subject.meshCells, Cultured
 
dc.subject.meshEpithelial Cells - Physiology
 
dc.subject.meshHumans
 
dc.subject.meshPeritoneum - Cytology
 
dc.titlePeritoneal mesothelial cell culture and biology
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Yung, S</contributor.author>
<contributor.author>Li, FK</contributor.author>
<contributor.author>Chan, TM</contributor.author>
<date.accessioned>2012-09-05T05:26:30Z</date.accessioned>
<date.available>2012-09-05T05:26:30Z</date.available>
<date.issued>2006</date.issued>
<identifier.citation>Peritoneal Dialysis International, 2006, v. 26 n. 2, p. 162-173</identifier.citation>
<identifier.issn>0896-8608</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/163010</identifier.uri>
<description.abstract>The peritoneal mesothelium is composed of an extensive monolayer of mesothelial cells that lines the body&apos;s serous cavity and internal organs and was previously thought to act principally as a protective nonadhesive lubricating surface to facilitate intracoelomic movement. With the introduction of peritoneal dialysis over three decades ago, there has been much interest in the cell biology of peritoneal mesothelial cells. Independent studies have highlighted specific properties of the peritoneal mesothelial cell, including antigen presentation, regenerative properties, clearance of fibrin; synthesis of cytokines, growth factors, and matrix proteins; and secretion of lubricants to protect the tissue from abrasion, adhesion, infection, and tumor dissemination. It is now evident that the mesothelium is not merely a passive membrane but, rather, a dynamic membrane that contributes substantially to the structural, functional, and homeostatic properties of the pentoneum. Since peritoneal mesothelial cells in culture possess immunohistochemical markers identical to mesothelial stem cells, the culture of mesothelial cells offers researchers an essential tool to assess their morphologic, structural, and functional properties. This review will discuss current procedures to isolate peritoneal mesothelial cells from human omental specimens, animal sources, and spent dialysate. Furthermore, the functional and morphologic properties of mesothelial cells are discussed, together with the potential use of mesothelial cell culture in research and clinical applications. Copyright &#169; 2006 International Society for Peritoneal Dialysis.</description.abstract>
<language>eng</language>
<publisher>Multimed, Inc. The Journal&apos;s web site is located at http://pdiconnect.com</publisher>
<relation.ispartof>Peritoneal Dialysis International</relation.ispartof>
<subject.mesh>Cell Culture Techniques - Methods</subject.mesh>
<subject.mesh>Cells, Cultured</subject.mesh>
<subject.mesh>Epithelial Cells - Physiology</subject.mesh>
<subject.mesh>Humans</subject.mesh>
<subject.mesh>Peritoneum - Cytology</subject.mesh>
<title>Peritoneal mesothelial cell culture and biology</title>
<type>Article</type>
<description.nature>Link_to_subscribed_fulltext</description.nature>
<identifier.pmid>16623418</identifier.pmid>
<identifier.scopus>eid_2-s2.0-33747135979</identifier.scopus>
<identifier.hkuros>117899</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-33747135979&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>26</identifier.volume>
<identifier.issue>2</identifier.issue>
<identifier.spage>162</identifier.spage>
<identifier.epage>173</identifier.epage>
<publisher.place>Canada</publisher.place>
</item>
Author Affiliations
  1. The University of Hong Kong