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Article: Proteomic analysis of adipocyte differentiation: Evidence that α2 macroglobulin is involved in the adipose conversion of 3T3 L1 preadipocytes

TitleProteomic analysis of adipocyte differentiation: Evidence that α2 macroglobulin is involved in the adipose conversion of 3T3 L1 preadipocytes
Authors
KeywordsAdipogenesis
Differentiation
Obesity
Protease Inhibitors
Two-dimensional gel electrophoresis
Issue Date2004
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomics
Citation
Proteomics, 2004, v. 4 n. 6, p. 1840-1848 How to Cite?
AbstractAdipogenesis is an important aspect of energy homeostasis. Here we have used a differential proteome mapping strategy to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3 L1 preadipocytes. Two-dimensional gel electrophoresis analysis identified 8 proteins that are induced following hormone-evoked differentiation. In addition, we found that a α2 macroglobulin fragment was abundantly present in 3T3 L1 preadipocytes, but was virtually undetectable in fully differentiated adipocytes. Metabolic radio-labeling with (35S)methionine and Northern blot analysis indicated that the intracellular α2 macroglobulin fragment in preadipocytes was derived from the extracellular culture medium, not de novo synthesis. Incubation of preadipocytes with an antiα2 macroglobulin polyclonal antibody caused depletion of the intracellular α2 macroglobulin fragments, and also enhanced spontaneous adipose conversion. These results suggest that intracellular α2 macroglobulin fragment inhibits adipocyte differentiation, and that hormone treatment induces differentiation at least in part by suppression of intracellular α2 macroglobulin activity in 3T3 L1 preadipocytes.
Persistent Identifierhttp://hdl.handle.net/10722/162912
ISSN
2015 Impact Factor: 4.079
2015 SCImago Journal Rankings: 1.476
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChoi, KLen_HK
dc.contributor.authorWang, Yen_HK
dc.contributor.authorTse, CAen_HK
dc.contributor.authorLam, KSLen_HK
dc.contributor.authorCooper, GJSen_HK
dc.contributor.authorXu, Aen_HK
dc.date.accessioned2012-09-05T05:25:11Z-
dc.date.available2012-09-05T05:25:11Z-
dc.date.issued2004en_HK
dc.identifier.citationProteomics, 2004, v. 4 n. 6, p. 1840-1848en_HK
dc.identifier.issn1615-9853en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162912-
dc.description.abstractAdipogenesis is an important aspect of energy homeostasis. Here we have used a differential proteome mapping strategy to identify intracellular proteins that are differentially expressed during adipose conversion of 3T3 L1 preadipocytes. Two-dimensional gel electrophoresis analysis identified 8 proteins that are induced following hormone-evoked differentiation. In addition, we found that a α2 macroglobulin fragment was abundantly present in 3T3 L1 preadipocytes, but was virtually undetectable in fully differentiated adipocytes. Metabolic radio-labeling with (35S)methionine and Northern blot analysis indicated that the intracellular α2 macroglobulin fragment in preadipocytes was derived from the extracellular culture medium, not de novo synthesis. Incubation of preadipocytes with an antiα2 macroglobulin polyclonal antibody caused depletion of the intracellular α2 macroglobulin fragments, and also enhanced spontaneous adipose conversion. These results suggest that intracellular α2 macroglobulin fragment inhibits adipocyte differentiation, and that hormone treatment induces differentiation at least in part by suppression of intracellular α2 macroglobulin activity in 3T3 L1 preadipocytes.en_HK
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.wiley-vch.de/home/proteomicsen_HK
dc.relation.ispartofProteomicsen_HK
dc.subjectAdipogenesisen_HK
dc.subjectDifferentiationen_HK
dc.subjectObesityen_HK
dc.subjectProtease Inhibitorsen_HK
dc.subjectTwo-dimensional gel electrophoresisen_HK
dc.subject.mesh3T3 Cellsen_US
dc.subject.meshAdipocytes - Physiologyen_US
dc.subject.meshAdipose Tissue - Metabolismen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Northernen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCulture Media - Chemistryen_US
dc.subject.meshElectrophoresis, Gel, Two-Dimensionalen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshMesenchymal Stem Cells - Chemistry - Physiologyen_US
dc.subject.meshMethionine - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMolecular Weighten_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshProteome - Analysisen_US
dc.subject.meshAlpha-Macroglobulins - Physiologyen_US
dc.titleProteomic analysis of adipocyte differentiation: Evidence that α2 macroglobulin is involved in the adipose conversion of 3T3 L1 preadipocytesen_HK
dc.typeArticleen_HK
dc.identifier.emailWang, Y: yuwanghk@hku.hken_HK
dc.identifier.emailLam, KSL: ksllam@hku.hken_HK
dc.identifier.emailXu, A: amxu@hkucc.hku.hken_HK
dc.identifier.authorityWang, Y=rp00239en_HK
dc.identifier.authorityLam, KSL=rp00343en_HK
dc.identifier.authorityXu, A=rp00485en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/pmic.200300697en_HK
dc.identifier.pmid15174150-
dc.identifier.scopuseid_2-s2.0-2942559000en_HK
dc.identifier.hkuros114928-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-2942559000&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume4en_HK
dc.identifier.issue6en_HK
dc.identifier.spage1840en_HK
dc.identifier.epage1848en_HK
dc.identifier.isiWOS:000221981200030-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridChoi, KL=7403949320en_HK
dc.identifier.scopusauthoridWang, Y=34973733700en_HK
dc.identifier.scopusauthoridTse, CA=7103295215en_HK
dc.identifier.scopusauthoridLam, KSL=8082870600en_HK
dc.identifier.scopusauthoridCooper, GJS=7402355946en_HK
dc.identifier.scopusauthoridXu, A=7202655409en_HK

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