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Article: Comparison of the COBAS TaqMan™ HBV test with the COBAS Amplicor Monitor™ Test for measurement of hepatitis B virus DNA in serum

TitleComparison of the COBAS TaqMan™ HBV test with the COBAS Amplicor Monitor™ Test for measurement of hepatitis B virus DNA in serum
Authors
Issue Date2005
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 2005, v. 77 n. 4, p. 486-490 How to Cite?
AbstractQuantitation of low hepatitis B virus (HBV) DNA levels in patients with chronic hepatitis B is important for monitoring natural history of disease and treatment efficacy. This study aimed to compare the quantitation range and analytical sensitivity of the newly developed COBAS TaqMan™ HBV test (TaqMan test) with the COBAS Amplicor™ HBV Monitor Test (Amplicor test), using the Eurohep HBV reference plasma and serum samples from patients. Serial dilutions (2.7 × 101-2.7 × 108 copies/ml) of the Eurohep HBV reference plasma and 50 serum samples from chronic hepatitis B patients were tested by both assays. The TaqMan test could detect seven (2.7 × 102-2.7 × 108 copies/ml) of eight dilutions of the reference plasma, while the Amplicor test could only detect three of them (2.7 × 103-2.7 × 105 copies/ml). The HBV DNA values measured by the TaqMan test correlated very well with the theoretical Eurohep standard values (r= 0.998, P < 0.001). There were good correlations between the HBV DNA levels measured by the two assays on both the Eurohep reference plasma (r = 0.993, P< 0.001) and serum samples from patients (r = 0.904, P< 0.001). Compared to the Amplicor test, the TaqMan test had a higher sensitivity (50 vs. 300 copies/ml), shorter assay time (6 vs. 10 hr), and wider dynamic range (8 vs. 3 logs), and was more cost-effective in a clinical setting. These data indicate that the TaqMan test is an excellent tool for HBV DNA quantitation. © 2005 Wiley-Liss, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/162901
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorSum, SSMen_US
dc.contributor.authorWong, DKHen_US
dc.contributor.authorYuen, JCHen_US
dc.contributor.authorLai, CLen_US
dc.contributor.authorYuen, MFen_US
dc.date.accessioned2012-09-05T05:25:05Z-
dc.date.available2012-09-05T05:25:05Z-
dc.date.issued2005en_US
dc.identifier.citationJournal Of Medical Virology, 2005, v. 77 n. 4, p. 486-490en_US
dc.identifier.issn0146-6615en_US
dc.identifier.urihttp://hdl.handle.net/10722/162901-
dc.description.abstractQuantitation of low hepatitis B virus (HBV) DNA levels in patients with chronic hepatitis B is important for monitoring natural history of disease and treatment efficacy. This study aimed to compare the quantitation range and analytical sensitivity of the newly developed COBAS TaqMan™ HBV test (TaqMan test) with the COBAS Amplicor™ HBV Monitor Test (Amplicor test), using the Eurohep HBV reference plasma and serum samples from patients. Serial dilutions (2.7 × 101-2.7 × 108 copies/ml) of the Eurohep HBV reference plasma and 50 serum samples from chronic hepatitis B patients were tested by both assays. The TaqMan test could detect seven (2.7 × 102-2.7 × 108 copies/ml) of eight dilutions of the reference plasma, while the Amplicor test could only detect three of them (2.7 × 103-2.7 × 105 copies/ml). The HBV DNA values measured by the TaqMan test correlated very well with the theoretical Eurohep standard values (r= 0.998, P < 0.001). There were good correlations between the HBV DNA levels measured by the two assays on both the Eurohep reference plasma (r = 0.993, P< 0.001) and serum samples from patients (r = 0.904, P< 0.001). Compared to the Amplicor test, the TaqMan test had a higher sensitivity (50 vs. 300 copies/ml), shorter assay time (6 vs. 10 hr), and wider dynamic range (8 vs. 3 logs), and was more cost-effective in a clinical setting. These data indicate that the TaqMan test is an excellent tool for HBV DNA quantitation. © 2005 Wiley-Liss, Inc.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_US
dc.relation.ispartofJournal of Medical Virologyen_US
dc.rightsJournal of Medical Virology. Copyright © John Wiley & Sons, Inc.-
dc.subject.meshAdolescenten_US
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAutomation - Instrumentation - Methodsen_US
dc.subject.meshDna Primersen_US
dc.subject.meshDna, Viral - Blooden_US
dc.subject.meshFemaleen_US
dc.subject.meshHepatitis B - Blood - Diagnosisen_US
dc.subject.meshHepatitis B Virus - Genetics - Isolation & Purification - Physiologyen_US
dc.subject.meshHumansen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPolymerase Chain Reaction - Methodsen_US
dc.subject.meshReagent Kits, Diagnosticen_US
dc.subject.meshSensitivity And Specificityen_US
dc.titleComparison of the COBAS TaqMan™ HBV test with the COBAS Amplicor Monitor™ Test for measurement of hepatitis B virus DNA in serumen_US
dc.typeArticleen_US
dc.identifier.emailWong, DKH:danywong@hku.hken_US
dc.identifier.emailLai, CL:hrmelcl@hku.hken_US
dc.identifier.emailYuen, MF:mfyuen@hkucc.hku.hken_US
dc.identifier.authorityWong, DKH=rp00492en_US
dc.identifier.authorityLai, CL=rp00314en_US
dc.identifier.authorityYuen, MF=rp00479en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jmv.20481en_US
dc.identifier.pmid16254975-
dc.identifier.scopuseid_2-s2.0-27744512499en_US
dc.identifier.hkuros115275-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-27744512499&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume77en_US
dc.identifier.issue4en_US
dc.identifier.spage486en_US
dc.identifier.epage490en_US
dc.identifier.isiWOS:000233128100006-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSum, SSM=6603889128en_US
dc.identifier.scopusauthoridWong, DKH=7401535819en_US
dc.identifier.scopusauthoridYuen, JCH=7102620480en_US
dc.identifier.scopusauthoridLai, CL=7403086396en_US
dc.identifier.scopusauthoridYuen, MF=7102031955en_US

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