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Article: Significance of monocytic cytokines at single cell level for the immune responsiveness in renal transplant recipients

TitleSignificance of monocytic cytokines at single cell level for the immune responsiveness in renal transplant recipients
Authors
KeywordsCytokines
Interleukin-10
Interleukin-6
Intracellular
Monocyte
Whole blood
Issue Date2002
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0934-0874&site=1
Citation
Transplant International, 2002, v. 15 n. 8, p. 415-420 How to Cite?
AbstractCytokine dysregulation is an important factor underlying the immune unresponsiveness to hepatitis B vaccination (HBV) in renal transplant recipients. This study investigated the relationship between monocyte-derived interleukin-6 (IL-6) and interleukin-10 (IL-10) production and the immune responsiveness using flow cytometry (cytoflow) after whole blood culture. According to their previous response to hepatitis B vaccination, 40 renal transplant recipients were divided into two groups of 20 patients. The percentage of CD14+ monocytes stained positive for intracellular IL-6 or IL-10 was measured using flow cytometry after 4 and 20 h of whole blood culture with lipopolysaccharide stimulation. The percentage of CD14+/IL-6+ cells after incubation in vitro for 4 h was lower in the responders compared to the non-responders and controls (27.15 ± 8.93 vs 35.47 ± 9.95, P = NS; and 37.06 ± 10.89, P < 0.05 respectively). The staining intensity of IL-6 at 4 h for responders was also significantly reduced. At 20 h, there were a significantly higher percentage of CD14+/IL-10+ positive cells in the responders compared to the non-responders (41.87 ± 18.39 vs 27.55 ± 17.25, P < 0.05). These results indicate that alteration of intracellular cytokine profile in activated monocytes distinguishes the HBV vaccination responders from the non-responders among renal transplant recipients. The capacity to upregulate monocyte IL-10 production in this subset of patients may modulate the immune responsiveness and effectively assists in mounting a positive response to HBV vaccination.
Persistent Identifierhttp://hdl.handle.net/10722/162649
ISSN
2015 Impact Factor: 2.835
2015 SCImago Journal Rankings: 1.107
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLi, FKen_HK
dc.contributor.authorHo, SKNen_HK
dc.contributor.authorYip, TPSen_HK
dc.contributor.authorLai, KNen_HK
dc.contributor.authorChan, TMen_HK
dc.date.accessioned2012-09-05T05:22:03Z-
dc.date.available2012-09-05T05:22:03Z-
dc.date.issued2002en_HK
dc.identifier.citationTransplant International, 2002, v. 15 n. 8, p. 415-420en_HK
dc.identifier.issn0934-0874en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162649-
dc.description.abstractCytokine dysregulation is an important factor underlying the immune unresponsiveness to hepatitis B vaccination (HBV) in renal transplant recipients. This study investigated the relationship between monocyte-derived interleukin-6 (IL-6) and interleukin-10 (IL-10) production and the immune responsiveness using flow cytometry (cytoflow) after whole blood culture. According to their previous response to hepatitis B vaccination, 40 renal transplant recipients were divided into two groups of 20 patients. The percentage of CD14+ monocytes stained positive for intracellular IL-6 or IL-10 was measured using flow cytometry after 4 and 20 h of whole blood culture with lipopolysaccharide stimulation. The percentage of CD14+/IL-6+ cells after incubation in vitro for 4 h was lower in the responders compared to the non-responders and controls (27.15 ± 8.93 vs 35.47 ± 9.95, P = NS; and 37.06 ± 10.89, P < 0.05 respectively). The staining intensity of IL-6 at 4 h for responders was also significantly reduced. At 20 h, there were a significantly higher percentage of CD14+/IL-10+ positive cells in the responders compared to the non-responders (41.87 ± 18.39 vs 27.55 ± 17.25, P < 0.05). These results indicate that alteration of intracellular cytokine profile in activated monocytes distinguishes the HBV vaccination responders from the non-responders among renal transplant recipients. The capacity to upregulate monocyte IL-10 production in this subset of patients may modulate the immune responsiveness and effectively assists in mounting a positive response to HBV vaccination.en_HK
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journal.asp?ref=0934-0874&site=1en_HK
dc.relation.ispartofTransplant Internationalen_HK
dc.rightsTransplant International. Copyright © Blackwell Publishing Ltd.-
dc.subjectCytokinesen_HK
dc.subjectInterleukin-10en_HK
dc.subjectInterleukin-6en_HK
dc.subjectIntracellularen_HK
dc.subjectMonocyteen_HK
dc.subjectWhole blooden_HK
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshAntigens, Cd14 - Blooden_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshInterleukin-10 - Biosynthesisen_US
dc.subject.meshInterleukin-6 - Biosynthesisen_US
dc.subject.meshKidney Transplantation - Immunologyen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshMonocytes - Metabolismen_US
dc.titleSignificance of monocytic cytokines at single cell level for the immune responsiveness in renal transplant recipientsen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailChan, TM: dtmchan@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityChan, TM=rp00394en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1432-2277.2002.tb00191.xen_HK
dc.identifier.pmid12221461-
dc.identifier.scopuseid_2-s2.0-0036942486en_HK
dc.identifier.hkuros79055-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0036942486&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume15en_HK
dc.identifier.issue8en_HK
dc.identifier.spage415en_HK
dc.identifier.epage420en_HK
dc.identifier.isiWOS:000178583600006-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLi, FK=8219093900en_HK
dc.identifier.scopusauthoridHo, SKN=36839065300en_HK
dc.identifier.scopusauthoridYip, TPS=7004283977en_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridChan, TM=7402687700en_HK

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