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Article: Influence of low density lipoprotein (LDL) subfraction profile and LDL oxidation on endothelium-dependent and independent vasodilation in patients with type 2 diabetes

TitleInfluence of low density lipoprotein (LDL) subfraction profile and LDL oxidation on endothelium-dependent and independent vasodilation in patients with type 2 diabetes
Authors
Issue Date1999
PublisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.org
Citation
Journal Of Clinical Endocrinology And Metabolism, 1999, v. 84 n. 9, p. 3212-3216 How to Cite?
AbstractRecent studies have suggested that hypercholesterolemia is associated with endothelial dysfunction. In patients with type 2 diabetes mellitus, dyslipidemia is mainly characterized by hypertriglyceridemia, low high density lipoprotein, and a preponderance of small dense low density lipoprotein (LDL) particles. We have examined the relationships among LDL subfractions, the susceptibility of LDL to oxidation in vitro, and endothelial function in type 2 diabetes mellitus. LDL subfractions were measured by density gradient ultracentrifugation. The susceptibility of LDL to oxidation was determined by measuring the kinetics of conjugated dienes formation during copper-mediated oxidation of LDL. Endothelium-dependent and independent vasodilation of the brachial artery were assessed by high resolution vascular ultrasound. Diabetic patients had a higher concentration of small dense LDL-III than matched controls (P <0.01). The lag phase of conjugated dienes formation was shorter in the diabetic patients (P < 0.05), and the rate of LDL oxidation was faster (P < 0.05). Both endothelium-dependent (P < 0.01) and independent dilation of the brachial artery (P < 0.01) were impaired in the diabetic patients. On multivariate analysis, the rate of oxidation and LDL-III concentration accounted for 12% and 6%, respectively, of the variation in endothelium-dependent vasodilation (adjusted r 2 = 0.18; P < 0.05), whereas LDL-III concentration and the maximum amount of conjugated dienes formed accounted for 27% and 5%, respectively, of the variation in endothelium-independent vasodilation (adjusted r 2 = 0.32; P < 0.01) in the diabetic patients. In conclusion, endothelial and smooth muscle cell dysfunction in type 2 diabetes were related to abnormalities in LDL subfractions and in LDL oxidation.
Persistent Identifierhttp://hdl.handle.net/10722/162357
ISSN
2014 Impact Factor: 6.209
2014 SCImago Journal Rankings: 2.625
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorTan, KCBen_US
dc.contributor.authorAi, VHGen_US
dc.contributor.authorChow, WSen_US
dc.contributor.authorChau, MTen_US
dc.contributor.authorLeong, Len_US
dc.contributor.authorLam, KSLen_US
dc.date.accessioned2012-09-05T05:19:16Z-
dc.date.available2012-09-05T05:19:16Z-
dc.date.issued1999en_US
dc.identifier.citationJournal Of Clinical Endocrinology And Metabolism, 1999, v. 84 n. 9, p. 3212-3216en_US
dc.identifier.issn0021-972Xen_US
dc.identifier.urihttp://hdl.handle.net/10722/162357-
dc.description.abstractRecent studies have suggested that hypercholesterolemia is associated with endothelial dysfunction. In patients with type 2 diabetes mellitus, dyslipidemia is mainly characterized by hypertriglyceridemia, low high density lipoprotein, and a preponderance of small dense low density lipoprotein (LDL) particles. We have examined the relationships among LDL subfractions, the susceptibility of LDL to oxidation in vitro, and endothelial function in type 2 diabetes mellitus. LDL subfractions were measured by density gradient ultracentrifugation. The susceptibility of LDL to oxidation was determined by measuring the kinetics of conjugated dienes formation during copper-mediated oxidation of LDL. Endothelium-dependent and independent vasodilation of the brachial artery were assessed by high resolution vascular ultrasound. Diabetic patients had a higher concentration of small dense LDL-III than matched controls (P <0.01). The lag phase of conjugated dienes formation was shorter in the diabetic patients (P < 0.05), and the rate of LDL oxidation was faster (P < 0.05). Both endothelium-dependent (P < 0.01) and independent dilation of the brachial artery (P < 0.01) were impaired in the diabetic patients. On multivariate analysis, the rate of oxidation and LDL-III concentration accounted for 12% and 6%, respectively, of the variation in endothelium-dependent vasodilation (adjusted r 2 = 0.18; P < 0.05), whereas LDL-III concentration and the maximum amount of conjugated dienes formed accounted for 27% and 5%, respectively, of the variation in endothelium-independent vasodilation (adjusted r 2 = 0.32; P < 0.01) in the diabetic patients. In conclusion, endothelial and smooth muscle cell dysfunction in type 2 diabetes were related to abnormalities in LDL subfractions and in LDL oxidation.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.orgen_US
dc.relation.ispartofJournal of Clinical Endocrinology and Metabolismen_US
dc.rightsJournal of Clinical Endocrinology and Metabolism. Copyright © The Endocrine Society.-
dc.subject.meshAdulten_US
dc.subject.meshBrachial Artery - Physiopathologyen_US
dc.subject.meshCentrifugation, Density Gradienten_US
dc.subject.meshCholesterol - Blooden_US
dc.subject.meshCopper - Chemistryen_US
dc.subject.meshDiabetes Mellitus, Type 2 - Blood - Physiopathologyen_US
dc.subject.meshEndothelium, Vascular - Physiopathologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshHemoglobin A, Glycosylated - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshKineticsen_US
dc.subject.meshLipid Peroxidationen_US
dc.subject.meshLipoproteins, Ldl - Blooden_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNitroglycerin - Pharmacologyen_US
dc.subject.meshVasodilationen_US
dc.subject.meshVasodilator Agents - Pharmacologyen_US
dc.titleInfluence of low density lipoprotein (LDL) subfraction profile and LDL oxidation on endothelium-dependent and independent vasodilation in patients with type 2 diabetesen_US
dc.typeArticleen_US
dc.identifier.emailTan, KCB:kcbtan@hku.hken_US
dc.identifier.emailLam, KSL:ksllam@hku.hken_US
dc.identifier.authorityTan, KCB=rp00402en_US
dc.identifier.authorityLam, KSL=rp00343en_US
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1210/jc.84.9.3212-
dc.identifier.pmid10487689-
dc.identifier.scopuseid_2-s2.0-0033304535en_US
dc.identifier.hkuros51185-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0033304535&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume84en_US
dc.identifier.issue9en_US
dc.identifier.spage3212en_US
dc.identifier.epage3216en_US
dc.identifier.isiWOS:000082360600035-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTan, KCB=8082703100en_US
dc.identifier.scopusauthoridAi, VHG=6603342063en_US
dc.identifier.scopusauthoridChow, WS=7402281153en_US
dc.identifier.scopusauthoridChau, MT=7006073758en_US
dc.identifier.scopusauthoridLeong, L=36853257400en_US
dc.identifier.scopusauthoridLam, KSL=8082870600en_US

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