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Article: Influence of low density lipoprotein (LDL) subfraction profile and LDL oxidation on endothelium-dependent and independent vasodilation in patients with type 2 diabetes
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TitleInfluence of low density lipoprotein (LDL) subfraction profile and LDL oxidation on endothelium-dependent and independent vasodilation in patients with type 2 diabetes
 
AuthorsTan, KCB1
Ai, VHG1
Chow, WS1
Chau, MT1
Leong, L1
Lam, KSL1
 
Issue Date1999
 
PublisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.org
 
CitationJournal Of Clinical Endocrinology And Metabolism, 1999, v. 84 n. 9, p. 3212-3216 [How to Cite?]
 
AbstractRecent studies have suggested that hypercholesterolemia is associated with endothelial dysfunction. In patients with type 2 diabetes mellitus, dyslipidemia is mainly characterized by hypertriglyceridemia, low high density lipoprotein, and a preponderance of small dense low density lipoprotein (LDL) particles. We have examined the relationships among LDL subfractions, the susceptibility of LDL to oxidation in vitro, and endothelial function in type 2 diabetes mellitus. LDL subfractions were measured by density gradient ultracentrifugation. The susceptibility of LDL to oxidation was determined by measuring the kinetics of conjugated dienes formation during copper-mediated oxidation of LDL. Endothelium-dependent and independent vasodilation of the brachial artery were assessed by high resolution vascular ultrasound. Diabetic patients had a higher concentration of small dense LDL-III than matched controls (P <0.01). The lag phase of conjugated dienes formation was shorter in the diabetic patients (P < 0.05), and the rate of LDL oxidation was faster (P < 0.05). Both endothelium-dependent (P < 0.01) and independent dilation of the brachial artery (P < 0.01) were impaired in the diabetic patients. On multivariate analysis, the rate of oxidation and LDL-III concentration accounted for 12% and 6%, respectively, of the variation in endothelium-dependent vasodilation (adjusted r 2 = 0.18; P < 0.05), whereas LDL-III concentration and the maximum amount of conjugated dienes formed accounted for 27% and 5%, respectively, of the variation in endothelium-independent vasodilation (adjusted r 2 = 0.32; P < 0.01) in the diabetic patients. In conclusion, endothelial and smooth muscle cell dysfunction in type 2 diabetes were related to abnormalities in LDL subfractions and in LDL oxidation.
 
ISSN0021-972X
2013 Impact Factor: 6.310
2013 SCImago Journal Rankings: 3.169
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorTan, KCB
 
dc.contributor.authorAi, VHG
 
dc.contributor.authorChow, WS
 
dc.contributor.authorChau, MT
 
dc.contributor.authorLeong, L
 
dc.contributor.authorLam, KSL
 
dc.date.accessioned2012-09-05T05:19:16Z
 
dc.date.available2012-09-05T05:19:16Z
 
dc.date.issued1999
 
dc.description.abstractRecent studies have suggested that hypercholesterolemia is associated with endothelial dysfunction. In patients with type 2 diabetes mellitus, dyslipidemia is mainly characterized by hypertriglyceridemia, low high density lipoprotein, and a preponderance of small dense low density lipoprotein (LDL) particles. We have examined the relationships among LDL subfractions, the susceptibility of LDL to oxidation in vitro, and endothelial function in type 2 diabetes mellitus. LDL subfractions were measured by density gradient ultracentrifugation. The susceptibility of LDL to oxidation was determined by measuring the kinetics of conjugated dienes formation during copper-mediated oxidation of LDL. Endothelium-dependent and independent vasodilation of the brachial artery were assessed by high resolution vascular ultrasound. Diabetic patients had a higher concentration of small dense LDL-III than matched controls (P <0.01). The lag phase of conjugated dienes formation was shorter in the diabetic patients (P < 0.05), and the rate of LDL oxidation was faster (P < 0.05). Both endothelium-dependent (P < 0.01) and independent dilation of the brachial artery (P < 0.01) were impaired in the diabetic patients. On multivariate analysis, the rate of oxidation and LDL-III concentration accounted for 12% and 6%, respectively, of the variation in endothelium-dependent vasodilation (adjusted r 2 = 0.18; P < 0.05), whereas LDL-III concentration and the maximum amount of conjugated dienes formed accounted for 27% and 5%, respectively, of the variation in endothelium-independent vasodilation (adjusted r 2 = 0.32; P < 0.01) in the diabetic patients. In conclusion, endothelial and smooth muscle cell dysfunction in type 2 diabetes were related to abnormalities in LDL subfractions and in LDL oxidation.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationJournal Of Clinical Endocrinology And Metabolism, 1999, v. 84 n. 9, p. 3212-3216 [How to Cite?]
 
dc.identifier.epage3216
 
dc.identifier.hkuros51185
 
dc.identifier.issn0021-972X
2013 Impact Factor: 6.310
2013 SCImago Journal Rankings: 3.169
 
dc.identifier.issue9
 
dc.identifier.pmid10487689
 
dc.identifier.scopuseid_2-s2.0-0033304535
 
dc.identifier.spage3212
 
dc.identifier.urihttp://hdl.handle.net/10722/162357
 
dc.identifier.volume84
 
dc.languageeng
 
dc.publisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.org
 
dc.publisher.placeUnited States
 
dc.relation.ispartofJournal of Clinical Endocrinology and Metabolism
 
dc.relation.referencesReferences in Scopus
 
dc.rightsJournal of Clinical Endocrinology and Metabolism. Copyright © The Endocrine Society.
 
dc.subject.meshAdult
 
dc.subject.meshBrachial Artery - Physiopathology
 
dc.subject.meshCentrifugation, Density Gradient
 
dc.subject.meshCholesterol - Blood
 
dc.subject.meshCopper - Chemistry
 
dc.subject.meshDiabetes Mellitus, Type 2 - Blood - Physiopathology
 
dc.subject.meshEndothelium, Vascular - Physiopathology
 
dc.subject.meshFemale
 
dc.subject.meshHemoglobin A, Glycosylated - Metabolism
 
dc.subject.meshHumans
 
dc.subject.meshKinetics
 
dc.subject.meshLipid Peroxidation
 
dc.subject.meshLipoproteins, Ldl - Blood
 
dc.subject.meshMale
 
dc.subject.meshMiddle Aged
 
dc.subject.meshNitroglycerin - Pharmacology
 
dc.subject.meshVasodilation
 
dc.subject.meshVasodilator Agents - Pharmacology
 
dc.titleInfluence of low density lipoprotein (LDL) subfraction profile and LDL oxidation on endothelium-dependent and independent vasodilation in patients with type 2 diabetes
 
dc.typeArticle
 
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<subject.mesh>Middle Aged</subject.mesh>
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Author Affiliations
  1. Queen Mary Hospital Hong Kong