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Article: Increased production of hyaluronan by peritoneal cells and its significance in patients on CAPD

TitleIncreased production of hyaluronan by peritoneal cells and its significance in patients on CAPD
Authors
KeywordsCAPD
Hyaluronan
Macrophages
Mesothelial cells
Peritoneal transport
Peritonitis
Issue Date1999
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/ajkd
Citation
American Journal Of Kidney Diseases, 1999, v. 33 n. 2, p. 318-324 How to Cite?
AbstractHyaluronan (HA) is a polysaccharide that forms a critical component of extracellular matrices. HA is present in high concentrations in tissues undergoing remodeling and morphogenesis, and it appears to have an important role in the early stages of wound healing. Here, we studied the level of HA in the peritoneal dialysate effluent (PDE) from 116 stable continuous ambulatory peritoneal dialysis (CAPD) patients. Longitudinal studies over a period of 6 weeks were performed in seven of these patients who developed peritonitis. The median HA level in PDE from these patients was 154.6 μg/L (range, 29.7 to 820.2 μg/L). Dialysate level of HA increased with age of the patients, but no such correlation was shown between HA level in PDE and duration of CAPD treatment or previous episodes of peritonitis. Patients with high or average peritoneal membrane transport of small solutes had a higher HA level in the PDE than those with a low peritoneal membrane transport (P = 0.046). A significant correlation was observed between PDE level of HA and interleukin-1β (IL-1β) or IL-6. The plasma level of HA in these patients was significantly greater than that of healthy controls (P < 0.0001), yet the plasma concentration of HA was only 85% that of the PDE concentration. In CAPD patients with peritonitis, there was a sharp increase in the PDE levels of HA, IL-1β, and IL-6. These values decreased progressively with resolution of peritonitis. The changes in the PDE levels of HA closely followed those of IL-1β or IL-6. In vitro [3H]-glucosamine incorporation studies suggest that the main bulk of HA is derived from synthesis by the peritoneal mesothelial cells, whereas the amount synthesized by macrophages is trivial. We conclude that elevated levels of HA found in the PDE of stable CAPD patients originate from increased synthesis by peritoneal mesothelial cells. This event may serve as a marker of regeneration and remodeling of the peritoneal lining.
Persistent Identifierhttp://hdl.handle.net/10722/162332
ISSN
2015 Impact Factor: 6.269
2015 SCImago Journal Rankings: 2.313
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLai, KNen_HK
dc.contributor.authorSzeto, CCen_HK
dc.contributor.authorLai, KBen_HK
dc.contributor.authorLam, CWKen_HK
dc.contributor.authorChan, DTMen_HK
dc.contributor.authorLeung, JCKen_HK
dc.date.accessioned2012-09-05T05:19:04Z-
dc.date.available2012-09-05T05:19:04Z-
dc.date.issued1999en_HK
dc.identifier.citationAmerican Journal Of Kidney Diseases, 1999, v. 33 n. 2, p. 318-324en_HK
dc.identifier.issn0272-6386en_HK
dc.identifier.urihttp://hdl.handle.net/10722/162332-
dc.description.abstractHyaluronan (HA) is a polysaccharide that forms a critical component of extracellular matrices. HA is present in high concentrations in tissues undergoing remodeling and morphogenesis, and it appears to have an important role in the early stages of wound healing. Here, we studied the level of HA in the peritoneal dialysate effluent (PDE) from 116 stable continuous ambulatory peritoneal dialysis (CAPD) patients. Longitudinal studies over a period of 6 weeks were performed in seven of these patients who developed peritonitis. The median HA level in PDE from these patients was 154.6 μg/L (range, 29.7 to 820.2 μg/L). Dialysate level of HA increased with age of the patients, but no such correlation was shown between HA level in PDE and duration of CAPD treatment or previous episodes of peritonitis. Patients with high or average peritoneal membrane transport of small solutes had a higher HA level in the PDE than those with a low peritoneal membrane transport (P = 0.046). A significant correlation was observed between PDE level of HA and interleukin-1β (IL-1β) or IL-6. The plasma level of HA in these patients was significantly greater than that of healthy controls (P < 0.0001), yet the plasma concentration of HA was only 85% that of the PDE concentration. In CAPD patients with peritonitis, there was a sharp increase in the PDE levels of HA, IL-1β, and IL-6. These values decreased progressively with resolution of peritonitis. The changes in the PDE levels of HA closely followed those of IL-1β or IL-6. In vitro [3H]-glucosamine incorporation studies suggest that the main bulk of HA is derived from synthesis by the peritoneal mesothelial cells, whereas the amount synthesized by macrophages is trivial. We conclude that elevated levels of HA found in the PDE of stable CAPD patients originate from increased synthesis by peritoneal mesothelial cells. This event may serve as a marker of regeneration and remodeling of the peritoneal lining.en_HK
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/ajkden_HK
dc.relation.ispartofAmerican Journal of Kidney Diseasesen_HK
dc.subjectCAPDen_HK
dc.subjectHyaluronanen_HK
dc.subjectMacrophagesen_HK
dc.subjectMesothelial cellsen_HK
dc.subjectPeritoneal transporten_HK
dc.subjectPeritonitisen_HK
dc.subject.meshAdulten_US
dc.subject.meshAgeden_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCross-Sectional Studiesen_US
dc.subject.meshEpithelial Cells - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshHumansen_US
dc.subject.meshHyaluronic Acid - Biosynthesis - Blood - Metabolismen_US
dc.subject.meshInterleukin-1 - Blooden_US
dc.subject.meshInterleukin-6 - Blooden_US
dc.subject.meshMacrophages, Peritoneal - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPeritoneal Cavity - Cytologyen_US
dc.subject.meshPeritoneal Dialysis, Continuous Ambulatory - Adverse Effectsen_US
dc.subject.meshPeritonitis - Blood - Etiology - Metabolismen_US
dc.titleIncreased production of hyaluronan by peritoneal cells and its significance in patients on CAPDen_HK
dc.typeArticleen_HK
dc.identifier.emailLai, KN: knlai@hku.hken_HK
dc.identifier.emailChan, DTM: dtmchan@hku.hken_HK
dc.identifier.emailLeung, JCK: jckleung@hku.hken_HK
dc.identifier.authorityLai, KN=rp00324en_HK
dc.identifier.authorityChan, DTM=rp00394en_HK
dc.identifier.authorityLeung, JCK=rp00448en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0272-6386(99)70307-0-
dc.identifier.pmid10023645-
dc.identifier.scopuseid_2-s2.0-0033006996en_HK
dc.identifier.hkuros41518-
dc.identifier.volume33en_HK
dc.identifier.issue2en_HK
dc.identifier.spage318en_HK
dc.identifier.epage324en_HK
dc.identifier.isiWOS:000078403100014-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLai, KN=7402135706en_HK
dc.identifier.scopusauthoridSzeto, CC=35495407200en_HK
dc.identifier.scopusauthoridLai, KB=7402135525en_HK
dc.identifier.scopusauthoridLam, CWK=8531362100en_HK
dc.identifier.scopusauthoridChan, DTM=7402687700en_HK
dc.identifier.scopusauthoridLeung, JCK=7202180349en_HK

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