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Article: Identification of endothelial cell membrane proteins that bind anti- DNA antibodies from patients with systemic lupus erythematosus by direct or indirect mechanisms

TitleIdentification of endothelial cell membrane proteins that bind anti- DNA antibodies from patients with systemic lupus erythematosus by direct or indirect mechanisms
Authors
Issue Date1997
PublisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjaut
Citation
Journal Of Autoimmunity, 1997, v. 10 n. 5, p. 433-439 How to Cite?
AbstractA subgroup of murine monoclonal anti-DNA antibodies bind to vascular endothelial cells either directly as a result of cross-reactivity, or indirectly through immunoglobulin-bound DNA and DNA-binding proteins on the endothelial cell membrane. To determine whether these mechanisms apply in human systemic lupus erythematosus (SLE), and to identify endothelial cell membrane protein(s) that bind human anti-DNA antibodies, we examined, by Western blotting, the binding of human polyclonal anti-DNA antibodies (PoAb) isolated from eight patients with SLE to human umbilical vein endothelial cell membrane proteins. PoAbs bind to endothelial membrane proteins With M(r) 84,000 and 46,000, which correspond to the DNA-binding proteins previously reported. Such binding is diminished after removal of DNA by DNase treatment. In addition, PoAbs bind to membrane proteins with M(r) 180,000, 110,000, 68,000, 44,000, and 35,000-30,000. Such binding is unaffected by alterations in DNA concentration. Anti-dsDNA and anti-ssDNA PoAbs from individual patients exhibit identical binding patterns, as are PoAbs isolated during active disease or remission. The results show that human anti-DNA antibodies can bind to endothelial cells both indirectly via immunoglobulin-bound DNA, and directly due to cross-reactivity. These mechanisms of cellular binding by anti-DNA antibodies may depict pathogenetic steps in human SLE.
Persistent Identifierhttp://hdl.handle.net/10722/162188
ISSN
2015 Impact Factor: 7.76
2015 SCImago Journal Rankings: 1.971
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorChan, TMen_US
dc.contributor.authorCheng, IKPen_US
dc.date.accessioned2012-09-05T05:17:54Z-
dc.date.available2012-09-05T05:17:54Z-
dc.date.issued1997en_US
dc.identifier.citationJournal Of Autoimmunity, 1997, v. 10 n. 5, p. 433-439en_US
dc.identifier.issn0896-8411en_US
dc.identifier.urihttp://hdl.handle.net/10722/162188-
dc.description.abstractA subgroup of murine monoclonal anti-DNA antibodies bind to vascular endothelial cells either directly as a result of cross-reactivity, or indirectly through immunoglobulin-bound DNA and DNA-binding proteins on the endothelial cell membrane. To determine whether these mechanisms apply in human systemic lupus erythematosus (SLE), and to identify endothelial cell membrane protein(s) that bind human anti-DNA antibodies, we examined, by Western blotting, the binding of human polyclonal anti-DNA antibodies (PoAb) isolated from eight patients with SLE to human umbilical vein endothelial cell membrane proteins. PoAbs bind to endothelial membrane proteins With M(r) 84,000 and 46,000, which correspond to the DNA-binding proteins previously reported. Such binding is diminished after removal of DNA by DNase treatment. In addition, PoAbs bind to membrane proteins with M(r) 180,000, 110,000, 68,000, 44,000, and 35,000-30,000. Such binding is unaffected by alterations in DNA concentration. Anti-dsDNA and anti-ssDNA PoAbs from individual patients exhibit identical binding patterns, as are PoAbs isolated during active disease or remission. The results show that human anti-DNA antibodies can bind to endothelial cells both indirectly via immunoglobulin-bound DNA, and directly due to cross-reactivity. These mechanisms of cellular binding by anti-DNA antibodies may depict pathogenetic steps in human SLE.en_US
dc.languageengen_US
dc.publisherAcademic Press. The Journal's web site is located at http://www.elsevier.com/locate/yjauten_US
dc.relation.ispartofJournal of Autoimmunityen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBlotting, Westernen_US
dc.subject.meshDna - Immunology - Metabolismen_US
dc.subject.meshDeoxyribonucleases - Metabolism - Pharmacologyen_US
dc.subject.meshEndothelium, Vascular - Immunology - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoblottingen_US
dc.subject.meshImmunoglobulin G - Blooden_US
dc.subject.meshLupus Erythematosus, Systemic - Blood - Immunologyen_US
dc.subject.meshMembrane Proteins - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshProtein Bindingen_US
dc.titleIdentification of endothelial cell membrane proteins that bind anti- DNA antibodies from patients with systemic lupus erythematosus by direct or indirect mechanismsen_US
dc.typeArticleen_US
dc.identifier.emailChan, TM:dtmchan@hku.hken_US
dc.identifier.authorityChan, TM=rp00394en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1006/jaut.1997.9998en_US
dc.identifier.pmid9376070-
dc.identifier.scopuseid_2-s2.0-0030880077en_US
dc.identifier.hkuros31522-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030880077&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume10en_US
dc.identifier.issue5en_US
dc.identifier.spage433en_US
dc.identifier.epage439en_US
dc.identifier.isiWOS:A1997YA16000002-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridChan, TM=7402687700en_US
dc.identifier.scopusauthoridCheng, IKP=7102537483en_US

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