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Article: Geographical distribution of hepatitis C virus genotypes in blood donors: An international collaborative survey

TitleGeographical distribution of hepatitis C virus genotypes in blood donors: An international collaborative survey
Authors
Issue Date1994
Citation
Journal Of Clinical Microbiology, 1994, v. 32 n. 4, p. 884-892 How to Cite?
AbstractThe frequency of infection with the six classified major genotypes of hepatitis C virus (HCV) was investigated in 447 infected volunteer blood donors from the following nine countries: Scotland, Finland, The Netherlands, Hungary, Australia, Egypt, Japan, Hong Kong, and Taiwan. Viral sequences in plasma from blood donors infected with HCV were amplified in the 5'-noncoding region and were typed by restriction fragment length polymorphism analysis. Electrophoresis of DNA fragments produced by cleavage with HaeIII-RsaI and ScrFI-HinfI allowed HCV types 1 (or 5), 2, 3, 4, and 6 to be identified. Further analysis with MvaI-HinfI allowed sequences of the type 5 genotype to be distinguished from sequences of type 1 genotype. Types 1, 2, and 3 accounted for almost all infections in donors from Scotland, Finland, The Netherlands, and Australia. Types 2 and 3 were not found in the eastern European country (Hungary), where all but one of the donors were infected with type 1. Donors from Japan and Taiwan were infected only with type 1 or 2, while types 1, 2, and 6 were found in those from Hong Kong. HCV infection among Egyptians was almost always by type 4. Donors infected with HCV type 1 showed broad serological reactivity with all four antigens of the second generation Chiron RIBA-2 assay (Chiron Corporation, Emeryville, Calif.), while infection with divergent HCV genotypes elicited antibodies mainly reactive to c22-3 and c33c. Reactivities with antibodies 5-1-1 and c100-3 were infrequent and were generally weak, irrespective of the geographical origin of the donor. Because the envelope region of HCV is even more variable than the NS-4 region, it is likely that vaccines based on these proteins need to be multivalent and perhaps specifically adapted for different geographical regions.
Persistent Identifierhttp://hdl.handle.net/10722/162042
ISSN
2015 Impact Factor: 3.631
2015 SCImago Journal Rankings: 2.151
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorSimmonds, Pen_US
dc.contributor.authorMcomish, Fen_US
dc.contributor.authorYap, PLen_US
dc.contributor.authorDow, BCen_US
dc.contributor.authorFollett, EACen_US
dc.contributor.authorSeed, Cen_US
dc.contributor.authorKeller, AJen_US
dc.contributor.authorCobain, TJen_US
dc.contributor.authorKrusius, Ten_US
dc.contributor.authorKolho, Een_US
dc.contributor.authorNaukkarinen, Ren_US
dc.contributor.authorLin, Cen_US
dc.contributor.authorLai, Cen_US
dc.contributor.authorLeong, Sen_US
dc.contributor.authorMedgyesi, GAen_US
dc.contributor.authorHejjas, Men_US
dc.contributor.authorKiyokawa, Hen_US
dc.contributor.authorFukada, Ken_US
dc.contributor.authorCuypers, Ten_US
dc.date.accessioned2012-09-05T05:16:51Z-
dc.date.available2012-09-05T05:16:51Z-
dc.date.issued1994en_US
dc.identifier.citationJournal Of Clinical Microbiology, 1994, v. 32 n. 4, p. 884-892en_US
dc.identifier.issn0095-1137en_US
dc.identifier.urihttp://hdl.handle.net/10722/162042-
dc.description.abstractThe frequency of infection with the six classified major genotypes of hepatitis C virus (HCV) was investigated in 447 infected volunteer blood donors from the following nine countries: Scotland, Finland, The Netherlands, Hungary, Australia, Egypt, Japan, Hong Kong, and Taiwan. Viral sequences in plasma from blood donors infected with HCV were amplified in the 5'-noncoding region and were typed by restriction fragment length polymorphism analysis. Electrophoresis of DNA fragments produced by cleavage with HaeIII-RsaI and ScrFI-HinfI allowed HCV types 1 (or 5), 2, 3, 4, and 6 to be identified. Further analysis with MvaI-HinfI allowed sequences of the type 5 genotype to be distinguished from sequences of type 1 genotype. Types 1, 2, and 3 accounted for almost all infections in donors from Scotland, Finland, The Netherlands, and Australia. Types 2 and 3 were not found in the eastern European country (Hungary), where all but one of the donors were infected with type 1. Donors from Japan and Taiwan were infected only with type 1 or 2, while types 1, 2, and 6 were found in those from Hong Kong. HCV infection among Egyptians was almost always by type 4. Donors infected with HCV type 1 showed broad serological reactivity with all four antigens of the second generation Chiron RIBA-2 assay (Chiron Corporation, Emeryville, Calif.), while infection with divergent HCV genotypes elicited antibodies mainly reactive to c22-3 and c33c. Reactivities with antibodies 5-1-1 and c100-3 were infrequent and were generally weak, irrespective of the geographical origin of the donor. Because the envelope region of HCV is even more variable than the NS-4 region, it is likely that vaccines based on these proteins need to be multivalent and perhaps specifically adapted for different geographical regions.en_US
dc.languageengen_US
dc.relation.ispartofJournal of Clinical Microbiologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshBlood Donorsen_US
dc.subject.meshBlood Transfusion - Adverse Effectsen_US
dc.subject.meshEgypt - Epidemiologyen_US
dc.subject.meshEurope - Epidemiologyen_US
dc.subject.meshFar East - Epidemiologyen_US
dc.subject.meshGenetic Variationen_US
dc.subject.meshGenotypeen_US
dc.subject.meshHepacivirus - Classification - Genetics - Isolation & Purificationen_US
dc.subject.meshHepatitis C - Epidemiology - Prevention & Controlen_US
dc.subject.meshHumansen_US
dc.subject.meshInternational Cooperationen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshPolymorphism, Restriction Fragment Lengthen_US
dc.subject.meshRna, Viral - Geneticsen_US
dc.titleGeographical distribution of hepatitis C virus genotypes in blood donors: An international collaborative surveyen_US
dc.typeArticleen_US
dc.identifier.emailLai, C:hrmelcl@hku.hken_US
dc.identifier.authorityLai, C=rp00314en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid7913097-
dc.identifier.scopuseid_2-s2.0-0028202422en_US
dc.identifier.volume32en_US
dc.identifier.issue4en_US
dc.identifier.spage884en_US
dc.identifier.epage892en_US
dc.identifier.isiWOS:A1994NB46900005-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridSimmonds, P=7102203090en_US
dc.identifier.scopusauthoridMcOmish, F=6603631027en_US
dc.identifier.scopusauthoridYap, PL=16938057500en_US
dc.identifier.scopusauthoridDow, BC=7004399846en_US
dc.identifier.scopusauthoridFollett, EAC=16941804300en_US
dc.identifier.scopusauthoridSeed, C=6602359324en_US
dc.identifier.scopusauthoridKeller, AJ=7401737648en_US
dc.identifier.scopusauthoridCobain, TJ=36966552800en_US
dc.identifier.scopusauthoridKrusius, T=7006105834en_US
dc.identifier.scopusauthoridKolho, E=6603018172en_US
dc.identifier.scopusauthoridNaukkarinen, R=6602423026en_US
dc.identifier.scopusauthoridLin, C=15034856400en_US
dc.identifier.scopusauthoridLai, C=7403086396en_US
dc.identifier.scopusauthoridLeong, S=54887049000en_US
dc.identifier.scopusauthoridMedgyesi, GA=35552456900en_US
dc.identifier.scopusauthoridHejjas, M=6602089412en_US
dc.identifier.scopusauthoridKiyokawa, H=7006511942en_US
dc.identifier.scopusauthoridFukada, K=55254895200en_US
dc.identifier.scopusauthoridCuypers, T=6602775228en_US

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