File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Prevalence of hepatitis C virus infection in hemodialysis patients: A longitudinal study comparing the results of RNA and antibody assays

TitlePrevalence of hepatitis C virus infection in hemodialysis patients: A longitudinal study comparing the results of RNA and antibody assays
Authors
Issue Date1993
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
Citation
Hepatology, 1993, v. 17 n. 1, p. 5-8 How to Cite?
AbstractWe longitudinally studied 51 patients from two hemodialysis centers to determine the prevalence of hepatitis C virus infection in hemodialysis patients. Serum samples were tested for antibody to HCV by first- and second- generation enzyme immunoassays and for hepatitis C virus RNA by nested polymerase chain reaction assay. Antibody to hepatitis C virus was detected in the initial serum samples by first-generation enzyme immunoassay and second-generation enzyme immunoassay in 6 (11.8%) and 11 (21.6%) patients, respectively. First-generation enzyme immunoassay had a false-positive rate of 33.3% and a false-negative rate of 63.6%. Hepatitis C virus RNA was found in eight second-generation enzyme immunoassay-positive patients (72.7%) and in one patient negative for antibody to hepatitis C virus (2.5%) giving an overall positivity rate of 17.6%. After 19 mo, antibody to hepatitis C virus was detected in 15 patients (29.4%) on second-generation enzyme immunoassay; hepatitis C virus RNA was found in 13 patients (25.5%). Hepatitis C virus markers persisted in all 12 patients with initial evidence of hepatitis C virus infection. Three patients acquired hepatitis C virus infection during the interim, giving a new infection rate of 4.9% per patient-year. Antibody to hepatitis C virus, hepatitis C virus RNA or both was detected in 55.6% of patients with biochemical changes suggestive of non-A, non-B hepatitis. Of the 15 antibody to hepatitis C virus, second-generation enzyme immunoassay- positive patients, 66.7% had persistently normal serum transaminase levels. In summary, hepatitis C virus infection is common among hemodialysis patients. First-generation enzyme immunoassay is an unreliable assay for antibody to hepatitis C virus in these patients. We found good correlation between the results of antibody to hepatitis C virus detection on second- generation enzyme immunoassay and of hepatitis C virus RNA by nested polymerase chain reaction and found that serum transaminase level is a poor indicator of hepatitis C virus infection in hemodialysis patients.
Persistent Identifierhttp://hdl.handle.net/10722/161988
ISSN
2015 Impact Factor: 11.711
2015 SCImago Journal Rankings: 4.752
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorTak Mao Chanen_US
dc.contributor.authorAnna Suk Fong Loken_US
dc.contributor.authorIgnatius Kum Po Chengen_US
dc.contributor.authorRachel Tzun Chanen_US
dc.date.accessioned2012-09-05T05:16:29Z-
dc.date.available2012-09-05T05:16:29Z-
dc.date.issued1993en_US
dc.identifier.citationHepatology, 1993, v. 17 n. 1, p. 5-8en_US
dc.identifier.issn0270-9139en_US
dc.identifier.urihttp://hdl.handle.net/10722/161988-
dc.description.abstractWe longitudinally studied 51 patients from two hemodialysis centers to determine the prevalence of hepatitis C virus infection in hemodialysis patients. Serum samples were tested for antibody to HCV by first- and second- generation enzyme immunoassays and for hepatitis C virus RNA by nested polymerase chain reaction assay. Antibody to hepatitis C virus was detected in the initial serum samples by first-generation enzyme immunoassay and second-generation enzyme immunoassay in 6 (11.8%) and 11 (21.6%) patients, respectively. First-generation enzyme immunoassay had a false-positive rate of 33.3% and a false-negative rate of 63.6%. Hepatitis C virus RNA was found in eight second-generation enzyme immunoassay-positive patients (72.7%) and in one patient negative for antibody to hepatitis C virus (2.5%) giving an overall positivity rate of 17.6%. After 19 mo, antibody to hepatitis C virus was detected in 15 patients (29.4%) on second-generation enzyme immunoassay; hepatitis C virus RNA was found in 13 patients (25.5%). Hepatitis C virus markers persisted in all 12 patients with initial evidence of hepatitis C virus infection. Three patients acquired hepatitis C virus infection during the interim, giving a new infection rate of 4.9% per patient-year. Antibody to hepatitis C virus, hepatitis C virus RNA or both was detected in 55.6% of patients with biochemical changes suggestive of non-A, non-B hepatitis. Of the 15 antibody to hepatitis C virus, second-generation enzyme immunoassay- positive patients, 66.7% had persistently normal serum transaminase levels. In summary, hepatitis C virus infection is common among hemodialysis patients. First-generation enzyme immunoassay is an unreliable assay for antibody to hepatitis C virus in these patients. We found good correlation between the results of antibody to hepatitis C virus detection on second- generation enzyme immunoassay and of hepatitis C virus RNA by nested polymerase chain reaction and found that serum transaminase level is a poor indicator of hepatitis C virus infection in hemodialysis patients.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/en_US
dc.relation.ispartofHepatologyen_US
dc.subject.meshAdulten_US
dc.subject.meshAlanine Transaminase - Blooden_US
dc.subject.meshCohort Studiesen_US
dc.subject.meshFemaleen_US
dc.subject.meshHepacivirus - Genetics - Immunologyen_US
dc.subject.meshHepatitis Antibodies - Analysisen_US
dc.subject.meshHepatitis C - Epidemiology - Immunology - Microbiologyen_US
dc.subject.meshHepatitis C Antibodiesen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunoenzyme Techniquesen_US
dc.subject.meshLongitudinal Studiesen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshPrevalenceen_US
dc.subject.meshRna, Viral - Analysisen_US
dc.subject.meshRenal Dialysisen_US
dc.titlePrevalence of hepatitis C virus infection in hemodialysis patients: A longitudinal study comparing the results of RNA and antibody assaysen_US
dc.typeArticleen_US
dc.identifier.emailTak Mao Chan:dtmchan@hku.hken_US
dc.identifier.authorityTak Mao Chan=rp00394en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0270-9139(93)90183-Nen_US
dc.identifier.pmid7678575-
dc.identifier.scopuseid_2-s2.0-0027392485en_US
dc.identifier.volume17en_US
dc.identifier.issue1en_US
dc.identifier.spage5en_US
dc.identifier.epage8en_US
dc.identifier.isiWOS:A1993KG67800002-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridTak Mao Chan=7402687700en_US
dc.identifier.scopusauthoridAnna Suk Fong Lok=35379868500en_US
dc.identifier.scopusauthoridIgnatius Kum Po Cheng=7409554683en_US
dc.identifier.scopusauthoridRachel Tzun Chan=7409884676en_US

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats