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Article: Antibody response to core, envelope and nonstructural hepatitis C virus antigens: Comparison of immunocompetent and immunosuppressed patients

TitleAntibody response to core, envelope and nonstructural hepatitis C virus antigens: Comparison of immunocompetent and immunosuppressed patients
Authors
Issue Date1993
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/
Citation
Hepatology, 1993, v. 18 n. 3, p. 497-502 How to Cite?
AbstractSome immunosuppressed patients with hepatitis C virus infection do not have detectable levels of antibody to hepatitis C virus on second-generation enzyme immunoassay. Antibodies to the envelope and nonstructural region 5 proteins have not been examined. Four groups of patients with hepatitis C virus infection were studied: (a) 20 immunocompetent patients, (b) 15 hemodialysis patients, (c) 17 kidney transplant recipients and (d) 3 acute leukemia patients who underwent bone marrow transplantation. Serum samples were tested for antibody to hepatitis C virus with a second-generation enzyme immunoassay and multiantigen enzyme immunoassays and for hepatitis C virus RNA with a nested polymerase chain reaction assay. All the immunocompetent patients reacted to C25, C22 and C33C; 90% reacted to nonstructural region 5 antigen and 80% reacted to C100-3. Only 55% reacted against yeast-derived e1 and e2 antigens, but all reacted against vaccinia virus-expressed N e1 and e2 antigens, indicating that the envelope epitopes are conformational and glycosylated. Sixty-five percent to 90% of dialysis and kidney transplant patients reacted to C25, C22 and N e1 and e2, but only 12% to 60% reacted to C100-3, C33C and nonstructural region 5 antigen. Diminution or loss of reactivity to hepatitis C virus antigens was observed after kidney and bone marrow transplantation, with C25 and N e1 and e2 less affected. Our data suggest that incorporation of C25 and N e1 and e2 antigens in the assay for antibody to hepatitis C virus would improve the detection of hepatitis C virus infection in immunosuppressed patients.
Persistent Identifierhttp://hdl.handle.net/10722/161964
ISSN
2015 Impact Factor: 11.711
2015 SCImago Journal Rankings: 4.752
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLok, ASFen_US
dc.contributor.authorChien, Den_US
dc.contributor.authorChoo, QLen_US
dc.contributor.authorChan, TMen_US
dc.contributor.authorChiu, EKWen_US
dc.contributor.authorCheng, IKPen_US
dc.contributor.authorHoughton, Men_US
dc.contributor.authorKuo, Gen_US
dc.date.accessioned2012-09-05T05:16:20Z-
dc.date.available2012-09-05T05:16:20Z-
dc.date.issued1993en_US
dc.identifier.citationHepatology, 1993, v. 18 n. 3, p. 497-502en_US
dc.identifier.issn0270-9139en_US
dc.identifier.urihttp://hdl.handle.net/10722/161964-
dc.description.abstractSome immunosuppressed patients with hepatitis C virus infection do not have detectable levels of antibody to hepatitis C virus on second-generation enzyme immunoassay. Antibodies to the envelope and nonstructural region 5 proteins have not been examined. Four groups of patients with hepatitis C virus infection were studied: (a) 20 immunocompetent patients, (b) 15 hemodialysis patients, (c) 17 kidney transplant recipients and (d) 3 acute leukemia patients who underwent bone marrow transplantation. Serum samples were tested for antibody to hepatitis C virus with a second-generation enzyme immunoassay and multiantigen enzyme immunoassays and for hepatitis C virus RNA with a nested polymerase chain reaction assay. All the immunocompetent patients reacted to C25, C22 and C33C; 90% reacted to nonstructural region 5 antigen and 80% reacted to C100-3. Only 55% reacted against yeast-derived e1 and e2 antigens, but all reacted against vaccinia virus-expressed N e1 and e2 antigens, indicating that the envelope epitopes are conformational and glycosylated. Sixty-five percent to 90% of dialysis and kidney transplant patients reacted to C25, C22 and N e1 and e2, but only 12% to 60% reacted to C100-3, C33C and nonstructural region 5 antigen. Diminution or loss of reactivity to hepatitis C virus antigens was observed after kidney and bone marrow transplantation, with C25 and N e1 and e2 less affected. Our data suggest that incorporation of C25 and N e1 and e2 antigens in the assay for antibody to hepatitis C virus would improve the detection of hepatitis C virus infection in immunosuppressed patients.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www.hepatology.org/en_US
dc.relation.ispartofHepatologyen_US
dc.subject.meshAntibody Formationen_US
dc.subject.meshAntigens, Viral - Immunologyen_US
dc.subject.meshBlood Transfusion - Adverse Effectsen_US
dc.subject.meshBone Marrow Transplantation - Immunologyen_US
dc.subject.meshHiv Seropositivityen_US
dc.subject.meshHepacivirus - Immunologyen_US
dc.subject.meshHepatitis Antibodies - Blooden_US
dc.subject.meshHepatitis C - Immunology - Transmissionen_US
dc.subject.meshHepatitis C Antibodiesen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunocompetenceen_US
dc.subject.meshImmunosuppressionen_US
dc.subject.meshKidney Transplantation - Immunologyen_US
dc.subject.meshLeukemia - Surgeryen_US
dc.subject.meshRenal Dialysisen_US
dc.subject.meshViral Core Proteins - Immunologyen_US
dc.subject.meshViral Envelope Proteins - Immunologyen_US
dc.subject.meshViral Nonstructural Proteins - Immunologyen_US
dc.titleAntibody response to core, envelope and nonstructural hepatitis C virus antigens: Comparison of immunocompetent and immunosuppressed patientsen_US
dc.typeArticleen_US
dc.identifier.emailChan, TM:dtmchan@hku.hken_US
dc.identifier.authorityChan, TM=rp00394en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/0270-9139(93)90347-Pen_US
dc.identifier.pmid7689528-
dc.identifier.scopuseid_2-s2.0-0027172231en_US
dc.identifier.volume18en_US
dc.identifier.issue3en_US
dc.identifier.spage497en_US
dc.identifier.epage502en_US
dc.identifier.isiWOS:A1993LV01800004-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLok, ASF=35379868500en_US
dc.identifier.scopusauthoridChien, D=7005297134en_US
dc.identifier.scopusauthoridChoo, QL=36501374700en_US
dc.identifier.scopusauthoridChan, TM=7402687700en_US
dc.identifier.scopusauthoridChiu, EKW=24827833600en_US
dc.identifier.scopusauthoridCheng, IKP=7102537483en_US
dc.identifier.scopusauthoridHoughton, M=7007018686en_US
dc.identifier.scopusauthoridKuo, G=16750292700en_US

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