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Article: Detection of supercoiled hepatitis B virus DNA and related forms by means of molecular hybridization to an oligonucleotide probe

TitleDetection of supercoiled hepatitis B virus DNA and related forms by means of molecular hybridization to an oligonucleotide probe
Authors
Issue Date1989
PublisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763
Citation
Journal Of Medical Virology, 1989, v. 29 n. 4, p. 284-288 How to Cite?
AbstractA novel assay for supercoiled and other fully double-stranded forms of hepatitis B virus (HBV) DNA in blood is presented that utilizes molecular hybridisation to a radiophosphorous-labeled oligonucleotide probe. The probe [5'-d(ACGTGCAGAGGTGAAGCGA)] is complementary to the S(+)-strand sequence furthest downstream, at the end of the gap. We examined the blood specimens from 137 healthy HBsAg-positive individuals, applying the probe to dots representing 2-3.5 ml serum or plasma. We found that supercoiled HBV is present in many HBV DNA-positiive blood specimens albeit in small quantities. Of the 104 specimens that were positive for HBV DNA of any form, 53 annealed to the probe. Serial specimens from the same subject taken over a period of months showed that the proportion of supercoil to other HBV DNA forms was variable. The presence of supercoil HBV DNA was not closely correlated with the level of serum HBV DNA polymerase. The supercoil is an HBV DNA form that can persist in the liver in the presence or absence of other replicative intermediates. This assay may enable further characterization of the status of HBV infection.
Persistent Identifierhttp://hdl.handle.net/10722/161809
ISSN
2015 Impact Factor: 1.998
2015 SCImago Journal Rankings: 1.015
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLin, HJen_US
dc.contributor.authorChung, HTen_US
dc.contributor.authorLai, CLen_US
dc.contributor.authorLeong, Sen_US
dc.contributor.authorTam, OSen_US
dc.date.accessioned2012-09-05T05:15:13Z-
dc.date.available2012-09-05T05:15:13Z-
dc.date.issued1989en_US
dc.identifier.citationJournal Of Medical Virology, 1989, v. 29 n. 4, p. 284-288en_US
dc.identifier.issn0146-6615en_US
dc.identifier.urihttp://hdl.handle.net/10722/161809-
dc.description.abstractA novel assay for supercoiled and other fully double-stranded forms of hepatitis B virus (HBV) DNA in blood is presented that utilizes molecular hybridisation to a radiophosphorous-labeled oligonucleotide probe. The probe [5'-d(ACGTGCAGAGGTGAAGCGA)] is complementary to the S(+)-strand sequence furthest downstream, at the end of the gap. We examined the blood specimens from 137 healthy HBsAg-positive individuals, applying the probe to dots representing 2-3.5 ml serum or plasma. We found that supercoiled HBV is present in many HBV DNA-positiive blood specimens albeit in small quantities. Of the 104 specimens that were positive for HBV DNA of any form, 53 annealed to the probe. Serial specimens from the same subject taken over a period of months showed that the proportion of supercoil to other HBV DNA forms was variable. The presence of supercoil HBV DNA was not closely correlated with the level of serum HBV DNA polymerase. The supercoil is an HBV DNA form that can persist in the liver in the presence or absence of other replicative intermediates. This assay may enable further characterization of the status of HBV infection.en_US
dc.languageengen_US
dc.publisherJohn Wiley & Sons, Inc. The Journal's web site is located at http://www3.interscience.wiley.com/cgi-bin/jhome/32763en_US
dc.relation.ispartofJournal of Medical Virologyen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshChild, Preschoolen_US
dc.subject.meshDna, Superhelical - Analysisen_US
dc.subject.meshDna, Viral - Blooden_US
dc.subject.meshDna-Directed Dna Polymerase - Blooden_US
dc.subject.meshElectrophoresis, Agar Gelen_US
dc.subject.meshHepatitis B Surface Antigens - Geneticsen_US
dc.subject.meshHepatitis B Virus - Geneticsen_US
dc.subject.meshHumansen_US
dc.subject.meshInfanten_US
dc.subject.meshOligonucleotide Probesen_US
dc.subject.meshScintillation Countingen_US
dc.titleDetection of supercoiled hepatitis B virus DNA and related forms by means of molecular hybridization to an oligonucleotide probeen_US
dc.typeArticleen_US
dc.identifier.emailLai, CL:hrmelcl@hku.hken_US
dc.identifier.authorityLai, CL=rp00314en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/jmv.1890290412en_US
dc.identifier.pmid2621454-
dc.identifier.scopuseid_2-s2.0-0024810927en_US
dc.identifier.volume29en_US
dc.identifier.issue4en_US
dc.identifier.spage284en_US
dc.identifier.epage288en_US
dc.identifier.isiWOS:A1989CE84200011-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLin, HJ=7405571292en_US
dc.identifier.scopusauthoridChung, HT=36797189000en_US
dc.identifier.scopusauthoridLai, CL=7403086396en_US
dc.identifier.scopusauthoridLeong, S=7201555322en_US
dc.identifier.scopusauthoridTam, OS=36794479300en_US

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