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Article: Vasoactive intestinal peptide in the anterior pituitary is increased in hypothyroidism

TitleVasoactive intestinal peptide in the anterior pituitary is increased in hypothyroidism
Authors
Issue Date1989
PublisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.org
Citation
Endocrinology, 1989, v. 124 n. 2, p. 1077-1084 How to Cite?
AbstractVasoactive intestinal peptide (VIP) and PRL have been reported to be colocalized in rat lactotropes. To determine whether induced hypothyroidism, known to reduce pituitary PRL concentration, also reduces pituitary concentration of VIP, rats were treated with antithyroid drugs for 3 weeks. Pituitary PRL concentration in male rats (μg/mg protein) was markedly reduced by this treatment (9.3 ± 1.0 vs. 2.3 ± 0.4 when extracted at pH 1.1, 17.9 ± 3.0 vs. 3.4 ± 0.4 when extracted at pH 7.4, 21.8 ± 3.3 vs. 6.7 ± 1.3 when extracted at pH 10.0). Contrary to expectation, pituitary VIP concentration was markedly increased in hypothyroidism; in males from 169.5 ± 20.3 to 834.0 ± 82.2 pg/mg protein, and in females (whose pituitary PRL had been similarly reduced) from 103.1/I ± 34 to 771.6 ± 100.9 pg/mg protein. Serum PRL was significantly reduced in hypothyroid males (7.4 ± 1.6 vs. 28.9 ± 12.2 ng/ml) whereas in females, serum PRL was not significantly altered (41.4 ± 11.6 vs 38.8 ± 14.3 ng/ml). The effect of hypothyroidism was reversed by administration of T 4 in physiological doses. The authenticity of pituitary immunoreactive VIP was further established by demonstrating chromatographic patterns by Sephadex G-50 gel exclusion and reverse phase HPLC separations identical to synthetic VIP. Immunohistochemically reactive VIP cells could not be demonstrated in normal pituitaries, but the marked increase in VIP in hypothyroid animals made it possible to visualize a population of VIP immunoreactive stellate cells which appear to be distinct from hypothyroid lactotropes and thyrotropes.
Persistent Identifierhttp://hdl.handle.net/10722/161799
ISSN
2015 Impact Factor: 4.159
2015 SCImago Journal Rankings: 2.363
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLam, KSLen_US
dc.contributor.authorLechan, RMen_US
dc.contributor.authorMinamitani, Nen_US
dc.contributor.authorSegerson, TPen_US
dc.contributor.authorReichlin, Sen_US
dc.date.accessioned2012-09-05T05:15:06Z-
dc.date.available2012-09-05T05:15:06Z-
dc.date.issued1989en_US
dc.identifier.citationEndocrinology, 1989, v. 124 n. 2, p. 1077-1084en_US
dc.identifier.issn0013-7227en_US
dc.identifier.urihttp://hdl.handle.net/10722/161799-
dc.description.abstractVasoactive intestinal peptide (VIP) and PRL have been reported to be colocalized in rat lactotropes. To determine whether induced hypothyroidism, known to reduce pituitary PRL concentration, also reduces pituitary concentration of VIP, rats were treated with antithyroid drugs for 3 weeks. Pituitary PRL concentration in male rats (μg/mg protein) was markedly reduced by this treatment (9.3 ± 1.0 vs. 2.3 ± 0.4 when extracted at pH 1.1, 17.9 ± 3.0 vs. 3.4 ± 0.4 when extracted at pH 7.4, 21.8 ± 3.3 vs. 6.7 ± 1.3 when extracted at pH 10.0). Contrary to expectation, pituitary VIP concentration was markedly increased in hypothyroidism; in males from 169.5 ± 20.3 to 834.0 ± 82.2 pg/mg protein, and in females (whose pituitary PRL had been similarly reduced) from 103.1/I ± 34 to 771.6 ± 100.9 pg/mg protein. Serum PRL was significantly reduced in hypothyroid males (7.4 ± 1.6 vs. 28.9 ± 12.2 ng/ml) whereas in females, serum PRL was not significantly altered (41.4 ± 11.6 vs 38.8 ± 14.3 ng/ml). The effect of hypothyroidism was reversed by administration of T 4 in physiological doses. The authenticity of pituitary immunoreactive VIP was further established by demonstrating chromatographic patterns by Sephadex G-50 gel exclusion and reverse phase HPLC separations identical to synthetic VIP. Immunohistochemically reactive VIP cells could not be demonstrated in normal pituitaries, but the marked increase in VIP in hypothyroid animals made it possible to visualize a population of VIP immunoreactive stellate cells which appear to be distinct from hypothyroid lactotropes and thyrotropes.en_US
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://endo.endojournals.orgen_US
dc.relation.ispartofEndocrinologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshFemaleen_US
dc.subject.meshHypothalamus - Metabolismen_US
dc.subject.meshHypothyroidism - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshPituitary Gland, Anterior - Drug Effects - Metabolismen_US
dc.subject.meshProlactin - Metabolismen_US
dc.subject.meshPropylthiouracil - Pharmacologyen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Strainsen_US
dc.subject.meshReference Valuesen_US
dc.subject.meshTriiodothyronine - Pharmacologyen_US
dc.subject.meshVasoactive Intestinal Peptide - Metabolismen_US
dc.titleVasoactive intestinal peptide in the anterior pituitary is increased in hypothyroidismen_US
dc.typeArticleen_US
dc.identifier.emailLam, KSL:ksllam@hku.hken_US
dc.identifier.authorityLam, KSL=rp00343en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/endo-124-2-1077-
dc.identifier.pmid2912685-
dc.identifier.scopuseid_2-s2.0-0024561404en_US
dc.identifier.volume124en_US
dc.identifier.issue2en_US
dc.identifier.spage1077en_US
dc.identifier.epage1084en_US
dc.identifier.isiWOS:A1989T063100071-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridLam, KSL=8082870600en_US
dc.identifier.scopusauthoridLechan, RM=7005636129en_US
dc.identifier.scopusauthoridMinamitani, N=6701500352en_US
dc.identifier.scopusauthoridSegerson, TP=55302295000en_US
dc.identifier.scopusauthoridReichlin, S=16186732300en_US

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