File Download

There are no files associated with this item.

  Links for fulltext
     (May Require Subscription)
  • Find via Find It@HKUL
Supplementary

Conference Paper: Decorin suppresses fibrotic processes in human peritoneal mesothelial cells through inhibition of PKC-alpha phosphorylation

TitleDecorin suppresses fibrotic processes in human peritoneal mesothelial cells through inhibition of PKC-alpha phosphorylation
Authors
KeywordsMedical sciences medical sciences
Urology and nephrology medical sciences
Experimental medicine, laboratory technique
Issue Date2012
PublisherMultimed, Inc. The Journal's web site is located at http://pdiconnect.com
Citation
The 14th Congress of the International Society for Peritoneal Dialysis (ISPD), Kuala Lumpur, Malaysia, 9-12 September 2012. In Peritoneal Dialysis International, 2012, v. 32 suppl. 3, p. S120 How to Cite?
AbstractOBJECTIVES: Mesothelial cell dysfunction contributes to peritoneal fibrosis during long-term peritoneal dialysis (PD). We previously reported that mesothelial cells synthesize decorin, a dermatan sulfate proteoglycan with anti-fibrotic properties. In this study, we investigated the mechanisms through which decorin regulates fibrogenesis in mesothelial cells in the setting of PD. METHODS: All experiments were conducted with human peritoneal mesothelial cells (HPMC), isolated from omental specimens by enzymatic digestion, at the second passage and growth arrested for 72 h. HPMC were stimulated with spent PD fluid, exogenous TGF-1 or IL-1 (10 ng/ml), in the presence or absence of TGF-1 neutralizing antibody (1 g/ml), IL-1 receptor antagonist (1 g/ml) or decorin (500 ng/ml) for 24 h. Cell morphology, its expression of fibronectin and -smooth muscle actin, and PKC-a activation was assessed. The role of decorin in fibrotic processes in HPMC was further investigated following gene silencing of decorin. RESULTS: Noninfected PDF induced epithelial-to-mesenchymal transition (EMT) and cell detachment in HPMC, and these phenotypic changes were exacerbated in cells exposed to infected PD fluid. Pre-incubation of HPMC with TGF-1 neutralizing antibody or IL-1 receptor antagonist inhibited PD fluid-induced EMT. Incubation of HPMC with exogenous decorin inhibited EMT induced by PD fluid, TGF-1 or IL-1. This was accompanied by decreased fibronectin synthesis and -smooth muscle actin expression. The effect of decorin was mediated through the suppression of PKC- phosphorylation. Knockdown of decorin expression in HPMC resulted in amplification of TGF-1-and IL-1-induced fibronectin synthesis and -smooth muscle actin expression. CONCLUSION: The data demonstrate that decorin ameliorates fibrotic processes in HPMC during PD through the suppression of PKC- signaling pathway.
DescriptionThis journal suppl. contain abstracts of the 14th Congress of the International Society for Peritoneal Dialysis 2012
Session: Basic Research on Biocompa tibility, Immunology, Inflammation and Fibrosis
Open Access Journal
Persistent Identifierhttp://hdl.handle.net/10722/160352
ISSN
2015 Impact Factor: 1.298
2015 SCImago Journal Rankings: 0.683

 

DC FieldValueLanguage
dc.contributor.authorChan, TMen_US
dc.contributor.authorChau, KMen_US
dc.contributor.authorYim, Aen_US
dc.contributor.authorZhang, Qen_US
dc.contributor.authorYung, Sen_US
dc.date.accessioned2012-08-16T06:08:11Z-
dc.date.available2012-08-16T06:08:11Z-
dc.date.issued2012en_US
dc.identifier.citationThe 14th Congress of the International Society for Peritoneal Dialysis (ISPD), Kuala Lumpur, Malaysia, 9-12 September 2012. In Peritoneal Dialysis International, 2012, v. 32 suppl. 3, p. S120en_US
dc.identifier.issn0896-8608-
dc.identifier.urihttp://hdl.handle.net/10722/160352-
dc.descriptionThis journal suppl. contain abstracts of the 14th Congress of the International Society for Peritoneal Dialysis 2012-
dc.descriptionSession: Basic Research on Biocompa tibility, Immunology, Inflammation and Fibrosis-
dc.descriptionOpen Access Journal-
dc.description.abstractOBJECTIVES: Mesothelial cell dysfunction contributes to peritoneal fibrosis during long-term peritoneal dialysis (PD). We previously reported that mesothelial cells synthesize decorin, a dermatan sulfate proteoglycan with anti-fibrotic properties. In this study, we investigated the mechanisms through which decorin regulates fibrogenesis in mesothelial cells in the setting of PD. METHODS: All experiments were conducted with human peritoneal mesothelial cells (HPMC), isolated from omental specimens by enzymatic digestion, at the second passage and growth arrested for 72 h. HPMC were stimulated with spent PD fluid, exogenous TGF-1 or IL-1 (10 ng/ml), in the presence or absence of TGF-1 neutralizing antibody (1 g/ml), IL-1 receptor antagonist (1 g/ml) or decorin (500 ng/ml) for 24 h. Cell morphology, its expression of fibronectin and -smooth muscle actin, and PKC-a activation was assessed. The role of decorin in fibrotic processes in HPMC was further investigated following gene silencing of decorin. RESULTS: Noninfected PDF induced epithelial-to-mesenchymal transition (EMT) and cell detachment in HPMC, and these phenotypic changes were exacerbated in cells exposed to infected PD fluid. Pre-incubation of HPMC with TGF-1 neutralizing antibody or IL-1 receptor antagonist inhibited PD fluid-induced EMT. Incubation of HPMC with exogenous decorin inhibited EMT induced by PD fluid, TGF-1 or IL-1. This was accompanied by decreased fibronectin synthesis and -smooth muscle actin expression. The effect of decorin was mediated through the suppression of PKC- phosphorylation. Knockdown of decorin expression in HPMC resulted in amplification of TGF-1-and IL-1-induced fibronectin synthesis and -smooth muscle actin expression. CONCLUSION: The data demonstrate that decorin ameliorates fibrotic processes in HPMC during PD through the suppression of PKC- signaling pathway.-
dc.languageengen_US
dc.publisherMultimed, Inc. The Journal's web site is located at http://pdiconnect.com-
dc.relation.ispartofPeritoneal Dialysis Internationalen_US
dc.subjectMedical sciences medical sciences-
dc.subjectUrology and nephrology medical sciences-
dc.subjectExperimental medicine, laboratory technique-
dc.titleDecorin suppresses fibrotic processes in human peritoneal mesothelial cells through inhibition of PKC-alpha phosphorylationen_US
dc.typeConference_Paperen_US
dc.identifier.emailChan, TM: dtmchan@hku.hken_US
dc.identifier.emailChau, KM: melchau@hkucc.hku.hken_US
dc.identifier.emailYim, A: anndyim@hku.hken_US
dc.identifier.emailZhang, Q: zhjhr@hkucc.hku.hken_US
dc.identifier.emailYung, S: ssyyung@hku.hken_US
dc.identifier.authorityChan, TM=rp00394en_US
dc.identifier.authorityYung, S=rp00455en_US
dc.identifier.hkuros205084en_US
dc.identifier.hkuros211198-
dc.identifier.volume32-
dc.identifier.issuesuppl. 3-
dc.identifier.spageS120-
dc.identifier.epageS120-
dc.publisher.placeCanada-
dc.customcontrol.immutablesml 20130614-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats