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Article: Role of miR-148a in hepatitis B associated hepatocellular carcinoma

TitleRole of miR-148a in hepatitis B associated hepatocellular carcinoma
Authors
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2012, v. 7 n. 4, article no. e35331 How to Cite?
AbstractHepatitis B virus encoded X antigen (HBx) is a trans-regulatory protein that alters the activity of selected transcription factors and cytoplasmic signal transduction pathways. HBx transcriptionally up-regulates the expression of a unique gene, URG11, which in turn transcriptionally up-regulates beta-catenin, thereby contributing importantly to hepatocarcinogenesis. HBx and URG11 also alter the expression of multiple microRNAs, and by miRNA array analysis, both were shown to promote the expression of miR-148a. Elevated miR-148a was also seen in HBx positive liver samples from infected patients. To study the function of miR-148a, anti-148a was introduced into HepG2 and Hep3B cells stably expressing HBx or stably over-expressing URG11. Anti-miR-148a suppressed cell proliferation, cell cycle progression, cell migration, anchorage independent growth in soft agar and subcutaneous tumor formation in SCID mice. Introduction of anti-miR-148a increased PTEN protein and mRNA expression, suggesting that PTEN was targeted by miR-148a. Anti-miR-148a failed to suppress PTEN expression when co-transfected with reporter gene mutants in the 3'UTR of PTEN mRNA. Introduction of anti-miR-148a also resulted in depressed Akt signaling by HBx and URG11, resulting in decreased expression of beta-catenin. Thus, miR-148a may play a central role in HBx/URG11 mediated HCC, and may be an early diagnostic marker and/or therapeutic target associated with this tumor type.
Persistent Identifierhttp://hdl.handle.net/10722/160061
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorYuan, Ken_US
dc.contributor.authorLian, Zen_US
dc.contributor.authorSun, Ben_US
dc.contributor.authorClayton, MMen_US
dc.contributor.authorNg, IOLen_US
dc.contributor.authorFeitelson, MAen_US
dc.date.accessioned2012-08-16T06:02:10Z-
dc.date.available2012-08-16T06:02:10Z-
dc.date.issued2012en_US
dc.identifier.citationPLoS One, 2012, v. 7 n. 4, article no. e35331en_US
dc.identifier.issn1932-6203en_US
dc.identifier.urihttp://hdl.handle.net/10722/160061-
dc.description.abstractHepatitis B virus encoded X antigen (HBx) is a trans-regulatory protein that alters the activity of selected transcription factors and cytoplasmic signal transduction pathways. HBx transcriptionally up-regulates the expression of a unique gene, URG11, which in turn transcriptionally up-regulates beta-catenin, thereby contributing importantly to hepatocarcinogenesis. HBx and URG11 also alter the expression of multiple microRNAs, and by miRNA array analysis, both were shown to promote the expression of miR-148a. Elevated miR-148a was also seen in HBx positive liver samples from infected patients. To study the function of miR-148a, anti-148a was introduced into HepG2 and Hep3B cells stably expressing HBx or stably over-expressing URG11. Anti-miR-148a suppressed cell proliferation, cell cycle progression, cell migration, anchorage independent growth in soft agar and subcutaneous tumor formation in SCID mice. Introduction of anti-miR-148a increased PTEN protein and mRNA expression, suggesting that PTEN was targeted by miR-148a. Anti-miR-148a failed to suppress PTEN expression when co-transfected with reporter gene mutants in the 3'UTR of PTEN mRNA. Introduction of anti-miR-148a also resulted in depressed Akt signaling by HBx and URG11, resulting in decreased expression of beta-catenin. Thus, miR-148a may play a central role in HBx/URG11 mediated HCC, and may be an early diagnostic marker and/or therapeutic target associated with this tumor type.-
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.mesh3' Untranslated Regions-
dc.subject.meshCarcinoma, Hepatocellular - diagnosis - virology-
dc.subject.meshHepatitis B - metabolism-
dc.subject.meshLiver Neoplasms - diagnosis - virology-
dc.subject.meshMicroRNAs - antagonists and inhibitors - metabolism-
dc.titleRole of miR-148a in hepatitis B associated hepatocellular carcinomaen_US
dc.typeArticleen_US
dc.identifier.openurlhttp://library.hku.hk:4550/resserv?sid=HKU:IR&issn=1932-6203 (Electronic) 1932-6203 (Linkin&volume=7&issue=4&spage=e35331&epage=&date=2012&atitle=Role+of+miR-148a+in+hepatitis+B+associated+hepatocellular+carcinomaen_US
dc.identifier.emailNg, IOL: iolng@hku.hken_US
dc.identifier.emailFeitelson, MA: feitelso@temple.edu-
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0035331-
dc.identifier.pmid22496917-
dc.identifier.pmcidPMC3322146-
dc.identifier.scopuseid_2-s2.0-84859499487-
dc.identifier.hkuros204790en_US
dc.identifier.volume7en_US
dc.identifier.issue4, article no. e35331en_US
dc.identifier.isiWOS:000305014500055-
dc.publisher.placeUnited States-

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