Article: CDK5RAP3 is a novel repressor of p14ARF in hepatocellular carcinoma cells

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TitleCDK5RAP3 is a novel repressor of p14ARF in hepatocellular carcinoma cells
AuthorsMak, GWY1
Lai, WL1
Zhou, Y1
Li, M3
Ng, IOL1 2
Ching, YP1 2
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
CitationPLoS One, 2012, v. 7 n. 7, article no. e42210 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0042210
AbstractCDK5 regulatory subunit associated protein 3 (CDK5RAP3) is a novel activator of PAK4 and processes important pro-metastatic function in hepatocarcinogenesis. However, it remains unclear if there are other mechanisms by which CDK5RAP3 promotes HCC metastasis. Here, we showed that in CDK5RAP3 stable knockdown SMMC-7721 HCC cells, p14(ARF) tumor suppressor was upregulated at protein and mRNA levels, and ectopic expression of CDK5RAP3 was found to repress the transcription of p14(ARF). Using chromatin immunoprecipitation assay, we demonstrated that CDK5RAP3 bound to p14(ARF) promoter in vivo. Furthermore, knockdown of p14(ARF) in CDK5RAP3 stable knockdown HCC cells reversed the suppression of HCC cell invasiveness mediated by knockdown of CDK5RAP3. Taken together, our findings provide the new evidence that overexpression of CDK5RAP3 promotes HCC metastasis via downregulation of p14(ARF).
ISSN1932-6203
2011 Impact Factor: 4.092
2011 SCImago Journal Rankings: 0.519
DOIhttp://dx.doi.org/10.1371/journal.pone.0042210
PubMed Central IDPMC3409131
DC Field
Value
dc.contributor.authorMak, GWY
dc.contributor.authorLai, WL
dc.contributor.authorZhou, Y
dc.contributor.authorLi, M
dc.contributor.authorNg, IOL
dc.contributor.authorChing, YP
dc.date.accessioned2012-08-16T05:47:31Z
dc.date.available2012-08-16T05:47:31Z
dc.date.issued2012
dc.description.abstractCDK5 regulatory subunit associated protein 3 (CDK5RAP3) is a novel activator of PAK4 and processes important pro-metastatic function in hepatocarcinogenesis. However, it remains unclear if there are other mechanisms by which CDK5RAP3 promotes HCC metastasis. Here, we showed that in CDK5RAP3 stable knockdown SMMC-7721 HCC cells, p14(ARF) tumor suppressor was upregulated at protein and mRNA levels, and ectopic expression of CDK5RAP3 was found to repress the transcription of p14(ARF). Using chromatin immunoprecipitation assay, we demonstrated that CDK5RAP3 bound to p14(ARF) promoter in vivo. Furthermore, knockdown of p14(ARF) in CDK5RAP3 stable knockdown HCC cells reversed the suppression of HCC cell invasiveness mediated by knockdown of CDK5RAP3. Taken together, our findings provide the new evidence that overexpression of CDK5RAP3 promotes HCC metastasis via downregulation of p14(ARF).
dc.description.naturepublished_or_final_version
dc.identifier.citationPLoS One, 2012, v. 7 n. 7, article no. e42210 [How to Cite?]
DOI: http://dx.doi.org/10.1371/journal.pone.0042210
dc.identifier.doihttp://dx.doi.org/10.1371/journal.pone.0042210
dc.identifier.hkuros202170
dc.identifier.issn1932-6203
2011 Impact Factor: 4.092
2011 SCImago Journal Rankings: 0.519
dc.identifier.issue7, article no. e42210
dc.identifier.pmcidPMC3409131
dc.identifier.pmid22860085
dc.identifier.scopuseid_2-s2.0-84864454197
dc.identifier.urihttp://hdl.handle.net/10722/159261
dc.identifier.volume7
dc.languageeng
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
dc.publisher.placeUnited States
dc.relation.ispartofPLoS One
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
dc.titleCDK5RAP3 is a novel repressor of p14ARF in hepatocellular carcinoma cells
dc.typeArticle
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
  3. Sun Yat-Sen University