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Article: CDK5RAP3 is a novel repressor of p14ARF in hepatocellular carcinoma cells

TitleCDK5RAP3 is a novel repressor of p14ARF in hepatocellular carcinoma cells
Authors
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
PLoS One, 2012, v. 7 n. 7, article no. e42210 How to Cite?
Abstract
CDK5 regulatory subunit associated protein 3 (CDK5RAP3) is a novel activator of PAK4 and processes important pro-metastatic function in hepatocarcinogenesis. However, it remains unclear if there are other mechanisms by which CDK5RAP3 promotes HCC metastasis. Here, we showed that in CDK5RAP3 stable knockdown SMMC-7721 HCC cells, p14(ARF) tumor suppressor was upregulated at protein and mRNA levels, and ectopic expression of CDK5RAP3 was found to repress the transcription of p14(ARF). Using chromatin immunoprecipitation assay, we demonstrated that CDK5RAP3 bound to p14(ARF) promoter in vivo. Furthermore, knockdown of p14(ARF) in CDK5RAP3 stable knockdown HCC cells reversed the suppression of HCC cell invasiveness mediated by knockdown of CDK5RAP3. Taken together, our findings provide the new evidence that overexpression of CDK5RAP3 promotes HCC metastasis via downregulation of p14(ARF).
Persistent Identifierhttp://hdl.handle.net/10722/159261
ISSN
2013 Impact Factor: 3.534
2013 SCImago Journal Rankings: 1.724
PubMed Central ID
ISI Accession Number ID

 

Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. The University of Hong Kong
  3. Sun Yat-Sen University
DC FieldValueLanguage
dc.contributor.authorMak, GWYen_US
dc.contributor.authorLai, WLen_US
dc.contributor.authorZhou, Yen_US
dc.contributor.authorLi, Men_US
dc.contributor.authorNg, IOLen_US
dc.contributor.authorChing, YPen_US
dc.date.accessioned2012-08-16T05:47:31Z-
dc.date.available2012-08-16T05:47:31Z-
dc.date.issued2012en_US
dc.identifier.citationPLoS One, 2012, v. 7 n. 7, article no. e42210en_US
dc.identifier.issn1932-6203-
dc.identifier.urihttp://hdl.handle.net/10722/159261-
dc.description.abstractCDK5 regulatory subunit associated protein 3 (CDK5RAP3) is a novel activator of PAK4 and processes important pro-metastatic function in hepatocarcinogenesis. However, it remains unclear if there are other mechanisms by which CDK5RAP3 promotes HCC metastasis. Here, we showed that in CDK5RAP3 stable knockdown SMMC-7721 HCC cells, p14(ARF) tumor suppressor was upregulated at protein and mRNA levels, and ectopic expression of CDK5RAP3 was found to repress the transcription of p14(ARF). Using chromatin immunoprecipitation assay, we demonstrated that CDK5RAP3 bound to p14(ARF) promoter in vivo. Furthermore, knockdown of p14(ARF) in CDK5RAP3 stable knockdown HCC cells reversed the suppression of HCC cell invasiveness mediated by knockdown of CDK5RAP3. Taken together, our findings provide the new evidence that overexpression of CDK5RAP3 promotes HCC metastasis via downregulation of p14(ARF).-
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action-
dc.relation.ispartofPLoS Oneen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.titleCDK5RAP3 is a novel repressor of p14ARF in hepatocellular carcinoma cellsen_US
dc.typeArticleen_US
dc.identifier.emailMak, GWY: gwymak@hku.hken_US
dc.identifier.emailLai, WL: bennywll@hku.hken_US
dc.identifier.emailZhou, Y: yzhou@hku.hken_US
dc.identifier.emailNg, IOL: iolng@hku.hken_US
dc.identifier.emailChing, YP: ypching@hku.hken_US
dc.identifier.authorityChing, YP=rp00469en_US
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0042210-
dc.identifier.pmid22860085-
dc.identifier.pmcidPMC3409131-
dc.identifier.scopuseid_2-s2.0-84864454197-
dc.identifier.hkuros202170en_US
dc.identifier.volume7-
dc.identifier.issue7, article no. e42210-
dc.identifier.isiWOS:000307045600074-
dc.publisher.placeUnited States-

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