Article: Comparative evaluation of a point-of-care immunochromatographic test SNAP 4Dx with molecular detection tests for vector-borne canine pathogens in Hong Kong

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TitleComparative evaluation of a point-of-care immunochromatographic test SNAP 4Dx with molecular detection tests for vector-borne canine pathogens in Hong Kong
AuthorsWong, SSY2
Teng, JLL2
Poon, RWS2
Choi, GKY2
Chan, KH2
Yeung, ML1
Hui, JJY3
Yuen, KY2
KeywordsAnaplasma Platys
Babesia
Dirofilaria Immitis
Ehrlichia
Wolbachia
Issue Date2011
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/vbz
CitationVector-Borne And Zoonotic Diseases, 2011, v. 11 n. 9, p. 1269-1277 [How to Cite?]
DOI: http://dx.doi.org/10.1089/vbz.2010.0265
AbstractThere are no comprehensive studies on the performance of commonly used point-of-care diagnostic enzyme immunoassay for common arthropod-borne canine pathogens. A comparative evaluation of an immunochromatographic test for these infections with a comprehensive polymerase chain reaction (PCR) test panel was performed on 100 pet dogs and 100 stray dogs without obvious clinical symptoms. Of the 162 positive test results from both immunochromatographic test and PCR, there was 85.2% concordance. The 24 discordant results between serology and PCR occurred in tests involving Ehrlichia canis (14) and Anaplasma platys (10), which may be related to the time of infection. No positive cases of borreliosis or rickettsiosis were detected. One important limitation of the immunochromatographic test was its lack of testing for babesiosis and hepatozoonosis. The former is the most prevalent arthropod-borne canine infection in our cohort (41%). Coinfections were found in 19% stray dogs and 6% of pet dogs with both tests (p<0.01). Seventeen and 8 samples from stray and pet dogs, respectively, were initially positive in the PCR test for Ehrlichia. However, on sequencing of the PCR amplicon, 10 from stray and 2 from pet dogs were found to be Wolbachia sequences instead, with 100% nucleotide identity to the 16S rRNA sequence of Wolbachia endosymbiont of Dirofilaria immitis. The presence of Wolbachia DNAemia (6%) correlated well with the molecular test and immunochromatographic antigen test for D. immitis. © Copyright 2011, Mary Ann Liebert, Inc.
ISSN1530-3667
2011 Impact Factor: 2.437
2011 SCImago Journal Rankings: 0.177
DOIhttp://dx.doi.org/10.1089/vbz.2010.0265
ISI Accession Number IDWOS:000294769900007
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorWong, SSY
dc.contributor.authorTeng, JLL
dc.contributor.authorPoon, RWS
dc.contributor.authorChoi, GKY
dc.contributor.authorChan, KH
dc.contributor.authorYeung, ML
dc.contributor.authorHui, JJY
dc.contributor.authorYuen, KY
dc.date.accessioned2012-08-08T08:51:53Z
dc.date.available2012-08-08T08:51:53Z
dc.date.issued2011
dc.description.abstractThere are no comprehensive studies on the performance of commonly used point-of-care diagnostic enzyme immunoassay for common arthropod-borne canine pathogens. A comparative evaluation of an immunochromatographic test for these infections with a comprehensive polymerase chain reaction (PCR) test panel was performed on 100 pet dogs and 100 stray dogs without obvious clinical symptoms. Of the 162 positive test results from both immunochromatographic test and PCR, there was 85.2% concordance. The 24 discordant results between serology and PCR occurred in tests involving Ehrlichia canis (14) and Anaplasma platys (10), which may be related to the time of infection. No positive cases of borreliosis or rickettsiosis were detected. One important limitation of the immunochromatographic test was its lack of testing for babesiosis and hepatozoonosis. The former is the most prevalent arthropod-borne canine infection in our cohort (41%). Coinfections were found in 19% stray dogs and 6% of pet dogs with both tests (p<0.01). Seventeen and 8 samples from stray and pet dogs, respectively, were initially positive in the PCR test for Ehrlichia. However, on sequencing of the PCR amplicon, 10 from stray and 2 from pet dogs were found to be Wolbachia sequences instead, with 100% nucleotide identity to the 16S rRNA sequence of Wolbachia endosymbiont of Dirofilaria immitis. The presence of Wolbachia DNAemia (6%) correlated well with the molecular test and immunochromatographic antigen test for D. immitis. © Copyright 2011, Mary Ann Liebert, Inc.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationVector-Borne And Zoonotic Diseases, 2011, v. 11 n. 9, p. 1269-1277 [How to Cite?]
DOI: http://dx.doi.org/10.1089/vbz.2010.0265
dc.identifier.doihttp://dx.doi.org/10.1089/vbz.2010.0265
dc.identifier.epage1277
dc.identifier.isiWOS:000294769900007
dc.identifier.issn1530-3667
2011 Impact Factor: 2.437
2011 SCImago Journal Rankings: 0.177
dc.identifier.issue9
dc.identifier.scopuseid_2-s2.0-80052323115
dc.identifier.spage1269
dc.identifier.urihttp://hdl.handle.net/10722/157642
dc.identifier.volume11
dc.languageeng
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/vbz
dc.publisher.placeUnited States
dc.relation.ispartofVector-Borne and Zoonotic Diseases
dc.relation.referencesReferences in Scopus
dc.subjectAnaplasma Platys
dc.subjectBabesia
dc.subjectDirofilaria Immitis
dc.subjectEhrlichia
dc.subjectWolbachia
dc.titleComparative evaluation of a point-of-care immunochromatographic test SNAP 4Dx with molecular detection tests for vector-borne canine pathogens in Hong Kong
dc.typeArticle
Author Affiliations
  1. Fisheries and Conservation Department
  2. The University of Hong Kong
  3. null