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Article: Comparative evaluation of a point-of-care immunochromatographic test SNAP 4Dx with molecular detection tests for vector-borne canine pathogens in Hong Kong

TitleComparative evaluation of a point-of-care immunochromatographic test SNAP 4Dx with molecular detection tests for vector-borne canine pathogens in Hong Kong
Authors
KeywordsAnaplasma Platys
Babesia
Dirofilaria Immitis
Ehrlichia
Wolbachia
Issue Date2011
PublisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/vbz
Citation
Vector-Borne And Zoonotic Diseases, 2011, v. 11 n. 9, p. 1269-1277 How to Cite?
Abstract
There are no comprehensive studies on the performance of commonly used point-of-care diagnostic enzyme immunoassay for common arthropod-borne canine pathogens. A comparative evaluation of an immunochromatographic test for these infections with a comprehensive polymerase chain reaction (PCR) test panel was performed on 100 pet dogs and 100 stray dogs without obvious clinical symptoms. Of the 162 positive test results from both immunochromatographic test and PCR, there was 85.2% concordance. The 24 discordant results between serology and PCR occurred in tests involving Ehrlichia canis (14) and Anaplasma platys (10), which may be related to the time of infection. No positive cases of borreliosis or rickettsiosis were detected. One important limitation of the immunochromatographic test was its lack of testing for babesiosis and hepatozoonosis. The former is the most prevalent arthropod-borne canine infection in our cohort (41%). Coinfections were found in 19% stray dogs and 6% of pet dogs with both tests (p<0.01). Seventeen and 8 samples from stray and pet dogs, respectively, were initially positive in the PCR test for Ehrlichia. However, on sequencing of the PCR amplicon, 10 from stray and 2 from pet dogs were found to be Wolbachia sequences instead, with 100% nucleotide identity to the 16S rRNA sequence of Wolbachia endosymbiont of Dirofilaria immitis. The presence of Wolbachia DNAemia (6%) correlated well with the molecular test and immunochromatographic antigen test for D. immitis. © Copyright 2011, Mary Ann Liebert, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/157642
ISSN
2013 Impact Factor: 2.531
2013 SCImago Journal Rankings: 1.172
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorWong, SSYen_US
dc.contributor.authorTeng, JLLen_US
dc.contributor.authorPoon, RWSen_US
dc.contributor.authorChoi, GKYen_US
dc.contributor.authorChan, KHen_US
dc.contributor.authorYeung, MLen_US
dc.contributor.authorHui, JJYen_US
dc.contributor.authorYuen, KYen_US
dc.date.accessioned2012-08-08T08:51:53Z-
dc.date.available2012-08-08T08:51:53Z-
dc.date.issued2011en_US
dc.identifier.citationVector-Borne And Zoonotic Diseases, 2011, v. 11 n. 9, p. 1269-1277en_US
dc.identifier.issn1530-3667en_US
dc.identifier.urihttp://hdl.handle.net/10722/157642-
dc.description.abstractThere are no comprehensive studies on the performance of commonly used point-of-care diagnostic enzyme immunoassay for common arthropod-borne canine pathogens. A comparative evaluation of an immunochromatographic test for these infections with a comprehensive polymerase chain reaction (PCR) test panel was performed on 100 pet dogs and 100 stray dogs without obvious clinical symptoms. Of the 162 positive test results from both immunochromatographic test and PCR, there was 85.2% concordance. The 24 discordant results between serology and PCR occurred in tests involving Ehrlichia canis (14) and Anaplasma platys (10), which may be related to the time of infection. No positive cases of borreliosis or rickettsiosis were detected. One important limitation of the immunochromatographic test was its lack of testing for babesiosis and hepatozoonosis. The former is the most prevalent arthropod-borne canine infection in our cohort (41%). Coinfections were found in 19% stray dogs and 6% of pet dogs with both tests (p<0.01). Seventeen and 8 samples from stray and pet dogs, respectively, were initially positive in the PCR test for Ehrlichia. However, on sequencing of the PCR amplicon, 10 from stray and 2 from pet dogs were found to be Wolbachia sequences instead, with 100% nucleotide identity to the 16S rRNA sequence of Wolbachia endosymbiont of Dirofilaria immitis. The presence of Wolbachia DNAemia (6%) correlated well with the molecular test and immunochromatographic antigen test for D. immitis. © Copyright 2011, Mary Ann Liebert, Inc.en_US
dc.languageengen_US
dc.publisherMary Ann Liebert, Inc Publishers. The Journal's web site is located at http://www.liebertpub.com/vbzen_US
dc.relation.ispartofVector-Borne and Zoonotic Diseasesen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.rightsThis is a copy of an article published in the [Vector-Borne And Zoonotic Diseases] © [2011] [copyright Mary Ann Liebert, Inc.]; [Vector-Borne And Zoonotic Diseases] is available online at: http://www.liebertonline.com.-
dc.subjectAnaplasma Platysen_US
dc.subjectBabesiaen_US
dc.subjectDirofilaria Immitisen_US
dc.subjectEhrlichiaen_US
dc.subjectWolbachiaen_US
dc.titleComparative evaluation of a point-of-care immunochromatographic test SNAP 4Dx with molecular detection tests for vector-borne canine pathogens in Hong Kongen_US
dc.typeArticleen_US
dc.identifier.emailWong, SSY:samsonsy@hkucc.hku.hken_US
dc.identifier.emailTeng, JLL:llteng@hku.hken_US
dc.identifier.emailYuen, KY:kyyuen@hkucc.hku.hken_US
dc.identifier.authorityWong, SSY=rp00395en_US
dc.identifier.authorityTeng, JLL=rp00277en_US
dc.identifier.authorityYuen, KY=rp00366en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1089/vbz.2010.0265en_US
dc.identifier.pmid21612526-
dc.identifier.scopuseid_2-s2.0-80052323115en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052323115&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume11en_US
dc.identifier.issue9en_US
dc.identifier.spage1269en_US
dc.identifier.epage1277en_US
dc.identifier.eissn1557-7759-
dc.identifier.isiWOS:000294769900007-
dc.publisher.placeUnited Statesen_US
dc.identifier.scopusauthoridWong, SSY=13310021400en_US
dc.identifier.scopusauthoridTeng, JLL=7202560229en_US
dc.identifier.scopusauthoridPoon, RWS=9334879200en_US
dc.identifier.scopusauthoridChoi, GKY=35423450200en_US
dc.identifier.scopusauthoridChan, KH=7406034307en_US
dc.identifier.scopusauthoridYeung, ML=50961890200en_US
dc.identifier.scopusauthoridHui, JJY=50961254900en_US
dc.identifier.scopusauthoridYuen, KY=36078079100en_US

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