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Article: Human epidermal growth factor excreted by recombinant Escherichia coli K-12 has the correct N-terminus and is fully bioactive

TitleHuman epidermal growth factor excreted by recombinant Escherichia coli K-12 has the correct N-terminus and is fully bioactive
Authors
KeywordsBioassay
Excretion
Fermentation
Hegf
Issue Date1999
PublisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbio
Citation
Process Biochemistry, 1999, v. 35 n. 1-2, p. 1-5 How to Cite?
AbstractThe high stability and productivity of recently developed Escherichia coli JM101 strains expressing human epidermal growth factor (hEGF) facilitated scale-up of hEGF production, and a protocol to purify hEGF from bacterial culture supernatant was developed, hEGF-containing supernatant from an induced hEGF-expressing recombinant E. coli culture was purified by: (A) QAE Sephadex A-25 ion-exchange chromatography; (B) Sephadex G-25 desalting; (C) SP-Sepharose cation-exchange chromatography; and (D) reverse-phase HPLC. The hEGF obtained was pure by HPLC and SDS-PAGE. The N-terminus of the purified hEGF was authentic. Commercial pure hEGF, and hEGF purified as described, were assessed for bioactivity, and yielded superimposable curves. The recovery of hEGF with this protocol was 30% of original, while the purity was 97-100%.
Persistent Identifierhttp://hdl.handle.net/10722/156510
ISSN
2015 Impact Factor: 2.529
2015 SCImago Journal Rankings: 0.937
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorHuang, RCen_US
dc.contributor.authorLam, Een_US
dc.contributor.authorChen, YHen_US
dc.contributor.authorHackett, Jen_US
dc.contributor.authorLam, TLen_US
dc.contributor.authorLiu, Den_US
dc.contributor.authorMa, MCen_US
dc.contributor.authorSiu, KLen_US
dc.contributor.authorSivakesava, Sen_US
dc.contributor.authorXu, ZNen_US
dc.contributor.authorWong, RSCen_US
dc.contributor.authorWong, WKRen_US
dc.date.accessioned2012-08-08T08:42:45Z-
dc.date.available2012-08-08T08:42:45Z-
dc.date.issued1999en_US
dc.identifier.citationProcess Biochemistry, 1999, v. 35 n. 1-2, p. 1-5en_US
dc.identifier.issn1359-5113en_US
dc.identifier.urihttp://hdl.handle.net/10722/156510-
dc.description.abstractThe high stability and productivity of recently developed Escherichia coli JM101 strains expressing human epidermal growth factor (hEGF) facilitated scale-up of hEGF production, and a protocol to purify hEGF from bacterial culture supernatant was developed, hEGF-containing supernatant from an induced hEGF-expressing recombinant E. coli culture was purified by: (A) QAE Sephadex A-25 ion-exchange chromatography; (B) Sephadex G-25 desalting; (C) SP-Sepharose cation-exchange chromatography; and (D) reverse-phase HPLC. The hEGF obtained was pure by HPLC and SDS-PAGE. The N-terminus of the purified hEGF was authentic. Commercial pure hEGF, and hEGF purified as described, were assessed for bioactivity, and yielded superimposable curves. The recovery of hEGF with this protocol was 30% of original, while the purity was 97-100%.en_US
dc.languageengen_US
dc.publisherElsevier Ltd. The Journal's web site is located at http://www.elsevier.com/locate/procbioen_US
dc.relation.ispartofProcess Biochemistryen_US
dc.subjectBioassayen_US
dc.subjectExcretionen_US
dc.subjectFermentationen_US
dc.subjectHegfen_US
dc.titleHuman epidermal growth factor excreted by recombinant Escherichia coli K-12 has the correct N-terminus and is fully bioactiveen_US
dc.typeArticleen_US
dc.identifier.emailChen, YH:yhchen@hkucc.hku.hken_US
dc.identifier.authorityChen, YH=rp00099en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1016/S0032-9592(99)00024-2en_US
dc.identifier.scopuseid_2-s2.0-0032716415en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032716415&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume35en_US
dc.identifier.issue1-2en_US
dc.identifier.spage1en_US
dc.identifier.epage5en_US
dc.identifier.isiWOS:000083520500001-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.scopusauthoridHuang, RC=7402950224en_US
dc.identifier.scopusauthoridLam, E=35959885400en_US
dc.identifier.scopusauthoridChen, YH=7601430448en_US
dc.identifier.scopusauthoridHackett, J=24302040000en_US
dc.identifier.scopusauthoridLam, TL=55046412000en_US
dc.identifier.scopusauthoridLiu, D=7410096329en_US
dc.identifier.scopusauthoridMa, MC=7202445069en_US
dc.identifier.scopusauthoridSiu, KL=36941388500en_US
dc.identifier.scopusauthoridSivakesava, S=6603079278en_US
dc.identifier.scopusauthoridXu, ZN=8416453800en_US
dc.identifier.scopusauthoridWong, RSC=7402126921en_US
dc.identifier.scopusauthoridWong, WKR=7403972071en_US

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