File Download
 
Links for fulltext
(May Require Subscription)
 
Supplementary

Article: Wnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: A microenvironment predisposed to tubal ectopic pregnancy
  • Basic View
  • Metadata View
  • XML View
TitleWnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: A microenvironment predisposed to tubal ectopic pregnancy
 
AuthorsKodithuwakku, SP1 2
Pang, RTK1
Ng, EHY1
Cheung, ANY1
Horne, AW3
Ho, PC1
Yeung, WSB1
Lee, KF1
 
KeywordsFallopian tube
olfactomedin-1
tubal ectopic pregnancy
Wnt signaling
 
Issue Date2012
 
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/labinvest/
 
CitationLaboratory Investigation, 2012, v. 92 n. 2, p. 256-264 [How to Cite?]
DOI: http://dx.doi.org/10.1038/labinvest.2011.148
 
AbstractEctopic pregnancy (EP) occurs when the embryo fails to transit to the uterus and attach to the luminal epithelium of the Fallopian tube (FT). Tubal EP is a common gynecological emergency and more than 95% of EP occurs in the ampullary region of the FT. In humans, Wnt activation and downregulation of olfactomedin-1 (Olfm-1) occur in the receptive endometrium and coincided with embryo implantation in vivo. Whether similar molecular changes happen in the FT leading to EP remains unclear. We hypothesized that activation of Wnt signaling downregulates Olfm-1 expression predisposes to EP. We investigated the spatiotemporal expression of Olfm-1 in FT from non-pregnant women and women with EP, and used a novel trophoblastic spheroid (embryo surrogate)-FT epithelial cell co-culture model (JAr and OE-E6/E7 cells) to study the role of Olfm-1 on spheroid attachment. Olfm-1 mRNA expression in the ampullary region of non-pregnant FT was higher (P0.05) in the follicular phase than in the luteal phase. Ampullary tubal Olfm-1 expression was lower in FT from women with EP compared to normal controls at the luteal phase (histological scoring (H-SCORE)1.30.2 vs 2.40.5; P0.05). Treatment of OE-E6/E7 with recombinant Olfm-1 (0.2-5 g/ml) suppressed spheroid attachment to OE-E6/E7 cells, while activation of Wnt-signaling pathway by Wnt3a or LiCl reduced endogenous Olfm-1 expression and increased spheroid attachment. Conversely, suppression of Olfm-1 expression by RNAi increased spheroid attachment to OE-E6/E7 cells. Taken together, Wnt activation suppresses Olfm-1 expression, and this may predispose a favorable microenvironment of the retained embryo in the FT, leading to EP in humans. © 2012 USCAP, Inc All rights reserved.
 
ISSN0023-6837
2013 Impact Factor: 3.828
 
DOIhttp://dx.doi.org/10.1038/labinvest.2011.148
 
PubMed Central IDPMC3272473
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorKodithuwakku, SP
 
dc.contributor.authorPang, RTK
 
dc.contributor.authorNg, EHY
 
dc.contributor.authorCheung, ANY
 
dc.contributor.authorHorne, AW
 
dc.contributor.authorHo, PC
 
dc.contributor.authorYeung, WSB
 
dc.contributor.authorLee, KF
 
dc.date.accessioned2012-07-16T09:52:57Z
 
dc.date.available2012-07-16T09:52:57Z
 
dc.date.issued2012
 
dc.description.abstractEctopic pregnancy (EP) occurs when the embryo fails to transit to the uterus and attach to the luminal epithelium of the Fallopian tube (FT). Tubal EP is a common gynecological emergency and more than 95% of EP occurs in the ampullary region of the FT. In humans, Wnt activation and downregulation of olfactomedin-1 (Olfm-1) occur in the receptive endometrium and coincided with embryo implantation in vivo. Whether similar molecular changes happen in the FT leading to EP remains unclear. We hypothesized that activation of Wnt signaling downregulates Olfm-1 expression predisposes to EP. We investigated the spatiotemporal expression of Olfm-1 in FT from non-pregnant women and women with EP, and used a novel trophoblastic spheroid (embryo surrogate)-FT epithelial cell co-culture model (JAr and OE-E6/E7 cells) to study the role of Olfm-1 on spheroid attachment. Olfm-1 mRNA expression in the ampullary region of non-pregnant FT was higher (P0.05) in the follicular phase than in the luteal phase. Ampullary tubal Olfm-1 expression was lower in FT from women with EP compared to normal controls at the luteal phase (histological scoring (H-SCORE)1.30.2 vs 2.40.5; P0.05). Treatment of OE-E6/E7 with recombinant Olfm-1 (0.2-5 g/ml) suppressed spheroid attachment to OE-E6/E7 cells, while activation of Wnt-signaling pathway by Wnt3a or LiCl reduced endogenous Olfm-1 expression and increased spheroid attachment. Conversely, suppression of Olfm-1 expression by RNAi increased spheroid attachment to OE-E6/E7 cells. Taken together, Wnt activation suppresses Olfm-1 expression, and this may predispose a favorable microenvironment of the retained embryo in the FT, leading to EP in humans. © 2012 USCAP, Inc All rights reserved.
 
dc.description.naturelink_to_OA_fulltext
 
dc.identifier.citationLaboratory Investigation, 2012, v. 92 n. 2, p. 256-264 [How to Cite?]
DOI: http://dx.doi.org/10.1038/labinvest.2011.148
 
dc.identifier.citeulike9866230
 
dc.identifier.doihttp://dx.doi.org/10.1038/labinvest.2011.148
 
dc.identifier.epage264
 
dc.identifier.hkuros201421
 
dc.identifier.hkuros211344
 
dc.identifier.hkuros227376
 
dc.identifier.issn0023-6837
2013 Impact Factor: 3.828
 
dc.identifier.issue2
 
dc.identifier.pmcidPMC3272473
 
dc.identifier.pmid21968811
 
dc.identifier.scopuseid_2-s2.0-84863063431
 
dc.identifier.spage256
 
dc.identifier.urihttp://hdl.handle.net/10722/152950
 
dc.identifier.volume92
 
dc.languageeng
 
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/labinvest/
 
dc.publisher.placeUnited Kingdom
 
dc.relation.ispartofLaboratory Investigation
 
dc.relation.referencesReferences in Scopus
 
dc.subject.meshExtracellular Matrix Proteins - physiology
 
dc.subject.meshFallopian Tubes - cytology - metabolism
 
dc.subject.meshGlycoproteins - physiology
 
dc.subject.meshPregnancy, Tubal - physiopathology
 
dc.subject.meshWnt Proteins - physiology
 
dc.subjectFallopian tube
 
dc.subjectolfactomedin-1
 
dc.subjecttubal ectopic pregnancy
 
dc.subjectWnt signaling
 
dc.titleWnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: A microenvironment predisposed to tubal ectopic pregnancy
 
dc.typeArticle
 
<?xml encoding="utf-8" version="1.0"?>
<item><contributor.author>Kodithuwakku, SP</contributor.author>
<contributor.author>Pang, RTK</contributor.author>
<contributor.author>Ng, EHY</contributor.author>
<contributor.author>Cheung, ANY</contributor.author>
<contributor.author>Horne, AW</contributor.author>
<contributor.author>Ho, PC</contributor.author>
<contributor.author>Yeung, WSB</contributor.author>
<contributor.author>Lee, KF</contributor.author>
<date.accessioned>2012-07-16T09:52:57Z</date.accessioned>
<date.available>2012-07-16T09:52:57Z</date.available>
<date.issued>2012</date.issued>
<identifier.citation>Laboratory Investigation, 2012, v. 92 n. 2, p. 256-264</identifier.citation>
<identifier.issn>0023-6837</identifier.issn>
<identifier.uri>http://hdl.handle.net/10722/152950</identifier.uri>
<description.abstract>Ectopic pregnancy (EP) occurs when the embryo fails to transit to the uterus and attach to the luminal epithelium of the Fallopian tube (FT). Tubal EP is a common gynecological emergency and more than 95% of EP occurs in the ampullary region of the FT. In humans, Wnt activation and downregulation of olfactomedin-1 (Olfm-1) occur in the receptive endometrium and coincided with embryo implantation in vivo. Whether similar molecular changes happen in the FT leading to EP remains unclear. We hypothesized that activation of Wnt signaling downregulates Olfm-1 expression predisposes to EP. We investigated the spatiotemporal expression of Olfm-1 in FT from non-pregnant women and women with EP, and used a novel trophoblastic spheroid (embryo surrogate)-FT epithelial cell co-culture model (JAr and OE-E6/E7 cells) to study the role of Olfm-1 on spheroid attachment. Olfm-1 mRNA expression in the ampullary region of non-pregnant FT was higher (P0.05) in the follicular phase than in the luteal phase. Ampullary tubal Olfm-1 expression was lower in FT from women with EP compared to normal controls at the luteal phase (histological scoring (H-SCORE)1.30.2 vs 2.40.5; P0.05). Treatment of OE-E6/E7 with recombinant Olfm-1 (0.2-5 g/ml) suppressed spheroid attachment to OE-E6/E7 cells, while activation of Wnt-signaling pathway by Wnt3a or LiCl reduced endogenous Olfm-1 expression and increased spheroid attachment. Conversely, suppression of Olfm-1 expression by RNAi increased spheroid attachment to OE-E6/E7 cells. Taken together, Wnt activation suppresses Olfm-1 expression, and this may predispose a favorable microenvironment of the retained embryo in the FT, leading to EP in humans. &#169; 2012 USCAP, Inc All rights reserved.</description.abstract>
<language>eng</language>
<publisher>Nature Publishing Group. The Journal&apos;s web site is located at http://www.nature.com/labinvest/</publisher>
<relation.ispartof>Laboratory Investigation</relation.ispartof>
<subject>Fallopian tube</subject>
<subject>olfactomedin-1</subject>
<subject>tubal ectopic pregnancy</subject>
<subject>Wnt signaling</subject>
<subject.mesh>Extracellular Matrix Proteins - physiology</subject.mesh>
<subject.mesh>Fallopian Tubes - cytology - metabolism</subject.mesh>
<subject.mesh>Glycoproteins - physiology</subject.mesh>
<subject.mesh>Pregnancy, Tubal - physiopathology</subject.mesh>
<subject.mesh>Wnt Proteins - physiology</subject.mesh>
<title>Wnt activation downregulates olfactomedin-1 in Fallopian tubal epithelial cells: A microenvironment predisposed to tubal ectopic pregnancy</title>
<type>Article</type>
<description.nature>link_to_OA_fulltext</description.nature>
<identifier.doi>10.1038/labinvest.2011.148</identifier.doi>
<identifier.pmid>21968811</identifier.pmid>
<identifier.pmcid>PMC3272473</identifier.pmcid>
<identifier.scopus>eid_2-s2.0-84863063431</identifier.scopus>
<identifier.hkuros>201421</identifier.hkuros>
<identifier.hkuros>211344</identifier.hkuros>
<identifier.hkuros>227376</identifier.hkuros>
<relation.references>http://www.scopus.com/mlt/select.url?eid=2-s2.0-84863063431&amp;selection=ref&amp;src=s&amp;origin=recordpage</relation.references>
<identifier.volume>92</identifier.volume>
<identifier.issue>2</identifier.issue>
<identifier.spage>256</identifier.spage>
<identifier.epage>264</identifier.epage>
<publisher.place>United Kingdom</publisher.place>
<identifier.citeulike>9866230</identifier.citeulike>
<bitstream.url>http://hub.hku.hk/bitstream/10722/152950/1/re01.htm</bitstream.url>
</item>
Author Affiliations
  1. The University of Hong Kong Li Ka Shing Faculty of Medicine
  2. University of Peradeniya
  3. University of Edinburgh