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Article: Ovarian stimulation modulates steroid receptor expression and spheroid attachment in peri-implantation endometria: Studies on natural and stimulated cycles

TitleOvarian stimulation modulates steroid receptor expression and spheroid attachment in peri-implantation endometria: Studies on natural and stimulated cycles
Authors
Keywordsendometrium
Steroid receptors
stimulated cycles
Issue Date2011
PublisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnstert
Citation
Fertility And Sterility, 2011, v. 96 n. 3, p. 764-768 How to Cite?
AbstractObjective: To compare the effect of high serum E 2 levels on endometrial steroid receptors in gonadotropin-stimulated cycles (hCG + 7) and natural cycles (LH + 7), and to study its effect on spheroid attachment. Design: Observational. Setting: University hospital. Patient(s): Infertile patient with normal menstrual cycles undergoing IVF treatment. Intervention(s): Gonadotropin stimulation and endometrial biopsy; trophoblast spheroid (embryo surrogate, Jeg-3)-endometrial cell (Ishikawa) coculture assay. Main Outcome Measure(s): Steroid receptor expression by quantitative polymerase chain reaction and immunohistochemistry; spheroid attachment rate. Result(s): Endometrial biopsies from natural (n = 12) and stimulated (n = 23) cycles were obtained. The expression of estrogen receptor α (ERα) but not ERβ or progesterone receptor (PR) transcript was significantly reduced in stimulated cycles compared with natural cycles. Glucocorticoid receptor (GR) transcript was significantly increased in the excessive responders of the stimulated cycle. There was no difference in ERα immunoreactivity in endometrial stroma, but a higher immunoreactivity was seen in endometrial glands of stimulated cycles. The endometrium of stimulated cycles had a lower expression of PR protein in glands, but a higher expression in stroma. Although no GR protein was detected in glands, GR protein expression was significantly up-regulated in stroma of the stimulated cycles. Endometrial cells treated with high steroid concentrations had a reduced spheroid attachment rate compared with the controls. Conclusion(s): High serum E 2 level affects the expression of steroid receptors in the endometrial cells and suppresses spheroid attachment. Copyright © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.
Persistent Identifierhttp://hdl.handle.net/10722/152938
ISSN
2015 Impact Factor: 4.426
2015 SCImago Journal Rankings: 2.156
ISI Accession Number ID
Funding AgencyGrant Number
Committee on Research and Conference Grants, University of Hong Kong
Funding Information:

Supported in part by grants from the Committee on Research and Conference Grants, University of Hong Kong.

References

 

DC FieldValueLanguage
dc.contributor.authorChai, Jen_HK
dc.contributor.authorLee, KFen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorHo, PCen_HK
dc.date.accessioned2012-07-16T09:52:50Z-
dc.date.available2012-07-16T09:52:50Z-
dc.date.issued2011en_HK
dc.identifier.citationFertility And Sterility, 2011, v. 96 n. 3, p. 764-768en_HK
dc.identifier.issn0015-0282en_HK
dc.identifier.urihttp://hdl.handle.net/10722/152938-
dc.description.abstractObjective: To compare the effect of high serum E 2 levels on endometrial steroid receptors in gonadotropin-stimulated cycles (hCG + 7) and natural cycles (LH + 7), and to study its effect on spheroid attachment. Design: Observational. Setting: University hospital. Patient(s): Infertile patient with normal menstrual cycles undergoing IVF treatment. Intervention(s): Gonadotropin stimulation and endometrial biopsy; trophoblast spheroid (embryo surrogate, Jeg-3)-endometrial cell (Ishikawa) coculture assay. Main Outcome Measure(s): Steroid receptor expression by quantitative polymerase chain reaction and immunohistochemistry; spheroid attachment rate. Result(s): Endometrial biopsies from natural (n = 12) and stimulated (n = 23) cycles were obtained. The expression of estrogen receptor α (ERα) but not ERβ or progesterone receptor (PR) transcript was significantly reduced in stimulated cycles compared with natural cycles. Glucocorticoid receptor (GR) transcript was significantly increased in the excessive responders of the stimulated cycle. There was no difference in ERα immunoreactivity in endometrial stroma, but a higher immunoreactivity was seen in endometrial glands of stimulated cycles. The endometrium of stimulated cycles had a lower expression of PR protein in glands, but a higher expression in stroma. Although no GR protein was detected in glands, GR protein expression was significantly up-regulated in stroma of the stimulated cycles. Endometrial cells treated with high steroid concentrations had a reduced spheroid attachment rate compared with the controls. Conclusion(s): High serum E 2 level affects the expression of steroid receptors in the endometrial cells and suppresses spheroid attachment. Copyright © 2011 American Society for Reproductive Medicine, Published by Elsevier Inc.en_HK
dc.languageengen_US
dc.publisherElsevier Inc. The Journal's web site is located at http://www.elsevier.com/locate/fertnsterten_HK
dc.relation.ispartofFertility and Sterilityen_HK
dc.subjectendometriumen_HK
dc.subjectSteroid receptorsen_HK
dc.subjectstimulated cyclesen_HK
dc.titleOvarian stimulation modulates steroid receptor expression and spheroid attachment in peri-implantation endometria: Studies on natural and stimulated cyclesen_HK
dc.typeArticleen_HK
dc.identifier.emailChai, J:jchai@hkucc.hku.hken_HK
dc.identifier.emailLee, KF:ckflee@hku.hken_HK
dc.identifier.emailNg, EHY:nghye@hkucc.hku.hken_HK
dc.identifier.emailYeung, WSB:wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailHo, PC:pcho@hku.hken_HK
dc.identifier.authorityChai, J=rp00241en_HK
dc.identifier.authorityLee, KF=rp00458en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.description.naturelink_to_subscribed_fulltext-
dc.identifier.doi10.1016/j.fertnstert.2011.06.015en_HK
dc.identifier.pmid21722890en_HK
dc.identifier.scopuseid_2-s2.0-80052268844en_HK
dc.identifier.hkuros200622en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-80052268844&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume96en_HK
dc.identifier.issue3en_HK
dc.identifier.spage764en_HK
dc.identifier.epage768en_HK
dc.identifier.eissn1556-5653-
dc.identifier.isiWOS:000294417000059-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridChai, J=35200414100en_HK
dc.identifier.scopusauthoridLee, KF=26643097500en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK

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