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Article: A garlic derivative, s-allylcysteine (sac), suppresses proliferation and metastasis of hepatocellular carcinoma

TitleA garlic derivative, s-allylcysteine (sac), suppresses proliferation and metastasis of hepatocellular carcinoma
Authors
Issue Date2012
PublisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.action
Citation
Plos One, 2012, v. 7 n. 2 How to Cite?
AbstractBackground: Hepatocellular carcinoma (HCC) is highly malignant and metastatic. Currently, there is no effective chemotherapy for patients with advanced HCC leading to an urgent need to seek for novel therapeutic options. We aimed to investigate the effect of a garlic derivative, S-allylcysteine (SAC), on the proliferation and metastasis of HCC. Methodology/Principal Findings: A series of in vitro experiments including MTT, colony-forming, wound-healing, invasion, apoptosis and cell cycle assays were performed to examine the anti-proliferative and anti-metastatic effects of SAC on a metastatic HCC cell line MHCC97L. The therapeutic values of SAC single and combined with cisplatin treatments were examined in an in vivo orthotopic xenograft liver tumor model. The result showed that the proliferation rate and colony-forming abilities of MHCC97L cells were suppressed by SAC together with significant suppression of the expressions of proliferation markers, Ki-67 and proliferating cell nuclear antigen (PCNA). Moreover, SAC hindered the migration and invasion of MHCC97L cells corresponding with up-regulation of E-cadherin and down-regulation of VEGF. Furthermore, SAC significantly induced apoptosis and necrosis of MHCC97L cells through suppressing Bcl-xL and Bcl-2 as well as activating caspase-3 and caspase-9. In addition, SAC could significantly induce the S phase arrest of MHCC97L cells together with down-regulation of cdc25c, cdc2 and cyclin B1. In vivo xenograft liver tumor model demonstrated that SAC single or combined with cisplatin treatment inhibited the progression and metastasis of HCC tumor. Conclusions/Significance: Our data demonstrate the anti-proliferative and anti-metastatic effects of SAC on HCC cells and suggest that SAC may be a potential therapeutic agent for the treatment of HCC patients. © 2012 Ng et al.
Persistent Identifierhttp://hdl.handle.net/10722/152871
ISSN
2015 Impact Factor: 3.057
2015 SCImago Journal Rankings: 1.395
PubMed Central ID
ISI Accession Number ID
Funding AgencyGrant Number
Small Project Funding
University of Hong Kong
Funding Information:

This study was supported by the Small Project Funding and Seed Funding from the University of Hong Kong. The funder had no role in study design, data collection and analysis, decision to publish, or preparation of the manuscript.

References

 

DC FieldValueLanguage
dc.contributor.authorNg, KTPen_HK
dc.contributor.authorGuo, DYen_HK
dc.contributor.authorCheng, Qen_HK
dc.contributor.authorGeng, Wen_HK
dc.contributor.authorLing, CCen_HK
dc.contributor.authorLi, CXen_HK
dc.contributor.authorLiu, XBen_HK
dc.contributor.authorMa, YYen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorPoon, RTPen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorMan, Ken_HK
dc.date.accessioned2012-07-16T09:51:03Z-
dc.date.available2012-07-16T09:51:03Z-
dc.date.issued2012en_HK
dc.identifier.citationPlos One, 2012, v. 7 n. 2en_HK
dc.identifier.issn1932-6203en_HK
dc.identifier.urihttp://hdl.handle.net/10722/152871-
dc.description.abstractBackground: Hepatocellular carcinoma (HCC) is highly malignant and metastatic. Currently, there is no effective chemotherapy for patients with advanced HCC leading to an urgent need to seek for novel therapeutic options. We aimed to investigate the effect of a garlic derivative, S-allylcysteine (SAC), on the proliferation and metastasis of HCC. Methodology/Principal Findings: A series of in vitro experiments including MTT, colony-forming, wound-healing, invasion, apoptosis and cell cycle assays were performed to examine the anti-proliferative and anti-metastatic effects of SAC on a metastatic HCC cell line MHCC97L. The therapeutic values of SAC single and combined with cisplatin treatments were examined in an in vivo orthotopic xenograft liver tumor model. The result showed that the proliferation rate and colony-forming abilities of MHCC97L cells were suppressed by SAC together with significant suppression of the expressions of proliferation markers, Ki-67 and proliferating cell nuclear antigen (PCNA). Moreover, SAC hindered the migration and invasion of MHCC97L cells corresponding with up-regulation of E-cadherin and down-regulation of VEGF. Furthermore, SAC significantly induced apoptosis and necrosis of MHCC97L cells through suppressing Bcl-xL and Bcl-2 as well as activating caspase-3 and caspase-9. In addition, SAC could significantly induce the S phase arrest of MHCC97L cells together with down-regulation of cdc25c, cdc2 and cyclin B1. In vivo xenograft liver tumor model demonstrated that SAC single or combined with cisplatin treatment inhibited the progression and metastasis of HCC tumor. Conclusions/Significance: Our data demonstrate the anti-proliferative and anti-metastatic effects of SAC on HCC cells and suggest that SAC may be a potential therapeutic agent for the treatment of HCC patients. © 2012 Ng et al.en_HK
dc.languageengen_US
dc.publisherPublic Library of Science. The Journal's web site is located at http://www.plosone.org/home.actionen_HK
dc.relation.ispartofPLoS ONEen_HK
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshCarcinoma, Hepatocellular - complications - drug therapy - metabolism-
dc.subject.meshCell Proliferation - drug effects-
dc.subject.meshCysteine - analogs and derivatives - pharmacology - therapeutic use-
dc.subject.meshGarlic - chemistry-
dc.subject.meshLiver Neoplasms - complications - drug therapy - metabolism-
dc.titleA garlic derivative, s-allylcysteine (sac), suppresses proliferation and metastasis of hepatocellular carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailNg, KTP: ledodes@hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.authorityNg, KTP=rp01720en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.description.naturepublished_or_final_version-
dc.identifier.doi10.1371/journal.pone.0031655en_HK
dc.identifier.pmid22389672-
dc.identifier.pmcidPMC3289621-
dc.identifier.scopuseid_2-s2.0-84863253114en_HK
dc.identifier.hkuros200782en_US
dc.identifier.hkuros206120-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84863253114&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume7en_HK
dc.identifier.issue2en_HK
dc.identifier.isiWOS:000302999600009-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridNg, KTP=7403178513en_HK
dc.identifier.scopusauthoridGuo, DY=36171425600en_HK
dc.identifier.scopusauthoridCheng, Q=16024087700en_HK
dc.identifier.scopusauthoridGeng, W=54412151800en_HK
dc.identifier.scopusauthoridLing, CC=55277226600en_HK
dc.identifier.scopusauthoridLi, CX=55277335900en_HK
dc.identifier.scopusauthoridLiu, XB=17435186900en_HK
dc.identifier.scopusauthoridMa, YY=55276973000en_HK
dc.identifier.scopusauthoridLo, CM=55261732500en_HK
dc.identifier.scopusauthoridPoon, RTP=55274361000en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK

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