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Article: Expression of adrenomedullin in human oviduct, its regulation by the hormonal cycle and contact with spermatozoa, and its effect on ciliary beat frequency of the oviductal epithelium

TitleExpression of adrenomedullin in human oviduct, its regulation by the hormonal cycle and contact with spermatozoa, and its effect on ciliary beat frequency of the oviductal epithelium
Authors
Issue Date2010
PublisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.org
Citation
Journal Of Clinical Endocrinology And Metabolism, 2010, v. 95 n. 9, p. E18-E25 How to Cite?
AbstractContext: Adrenomedullin (ADM) has been found expressed in the mouse oviduct and might play a role in reproduction. Objective: The objective of the study was to demonstrate the expression of ADM in the human oviduct, investigate its regulation by steroidal hormones and spermatozoa contact, and study its effect on ciliary beat frequency (CBF) in the human oviduct. Design, Setting, Patients, and Interventions: Oviducts from women undergoing hysterectomy for benign diseases in a university hospital were collected. The oviducts were treated with estradiol and/or progesterone to simulate different phases of the ovarian cycle. ADM expression was studied at the peptide and mRNA levels by immunohistochemistry and RT-PCR, respectively. CBF was measured after treatment with graded concentrations of ADM and its antagonists. Cells from OE-E6/E7, an immortalized oviductal cell line, as well as oviductal tissue were cocultured with and without direct contact with capacitated human spermatozoa to compare oviductal cell ADM expression levels. CBF was also analyzed in oviductal tissue after spermatozoa-oviduct coincubation. Results: ADM expression was the highest in the isthmic region and in a hormonal environment simulating the early luteal phase. CBF was increased by ADM in a dose-dependent manner, which wasblocked by ADM and calcitonin-gene-related peptide receptor antagonists. Direct contact with spermatozoa in coculture resulted in higher ADM expression in OE-E6/E7 cell line and oviductal tissue and higher CBF in oviductal epithelium. Conclusions: ADM expression in the human oviduct is hormone dependent and is up-regulated by sperm contact. ADM stimulates ciliary motility of the human oviduct. Copyright © 2010 by The Endocrine Society.
Persistent Identifierhttp://hdl.handle.net/10722/149747
ISSN
2015 Impact Factor: 5.531
2015 SCImago Journal Rankings: 2.940
ISI Accession Number ID
Funding AgencyGrant Number
Committee of Research and Conference
University of Hong Kong
Hong Kong Research Grant CouncilHKU7578/06M
Funding Information:

This work was supported by grants from the Committee of Research and Conference Grant and the University of Hong Kong, and Hong Kong Research Grant Council Grant HKU7578/06M.

References

 

DC FieldValueLanguage
dc.contributor.authorLi, HWRen_HK
dc.contributor.authorLiao, SBen_HK
dc.contributor.authorChiu, PCNen_HK
dc.contributor.authorTam, WWen_HK
dc.contributor.authorHo, JCen_HK
dc.contributor.authorNg, EHYen_HK
dc.contributor.authorHo, PCen_HK
dc.contributor.authorYeung, WSBen_HK
dc.contributor.authorTang, Fen_HK
dc.contributor.authorO, WSen_HK
dc.date.accessioned2012-06-26T05:57:56Z-
dc.date.available2012-06-26T05:57:56Z-
dc.date.issued2010en_HK
dc.identifier.citationJournal Of Clinical Endocrinology And Metabolism, 2010, v. 95 n. 9, p. E18-E25en_HK
dc.identifier.issn0021-972Xen_HK
dc.identifier.urihttp://hdl.handle.net/10722/149747-
dc.description.abstractContext: Adrenomedullin (ADM) has been found expressed in the mouse oviduct and might play a role in reproduction. Objective: The objective of the study was to demonstrate the expression of ADM in the human oviduct, investigate its regulation by steroidal hormones and spermatozoa contact, and study its effect on ciliary beat frequency (CBF) in the human oviduct. Design, Setting, Patients, and Interventions: Oviducts from women undergoing hysterectomy for benign diseases in a university hospital were collected. The oviducts were treated with estradiol and/or progesterone to simulate different phases of the ovarian cycle. ADM expression was studied at the peptide and mRNA levels by immunohistochemistry and RT-PCR, respectively. CBF was measured after treatment with graded concentrations of ADM and its antagonists. Cells from OE-E6/E7, an immortalized oviductal cell line, as well as oviductal tissue were cocultured with and without direct contact with capacitated human spermatozoa to compare oviductal cell ADM expression levels. CBF was also analyzed in oviductal tissue after spermatozoa-oviduct coincubation. Results: ADM expression was the highest in the isthmic region and in a hormonal environment simulating the early luteal phase. CBF was increased by ADM in a dose-dependent manner, which wasblocked by ADM and calcitonin-gene-related peptide receptor antagonists. Direct contact with spermatozoa in coculture resulted in higher ADM expression in OE-E6/E7 cell line and oviductal tissue and higher CBF in oviductal epithelium. Conclusions: ADM expression in the human oviduct is hormone dependent and is up-regulated by sperm contact. ADM stimulates ciliary motility of the human oviduct. Copyright © 2010 by The Endocrine Society.en_HK
dc.languageengen_US
dc.publisherThe Endocrine Society. The Journal's web site is located at http://jcem.endojournals.orgen_HK
dc.relation.ispartofJournal of Clinical Endocrinology and Metabolismen_HK
dc.subject.meshAdrenomedullin - Genetics - Metabolismen_US
dc.subject.meshCalcitonin Receptor-Like Proteinen_US
dc.subject.meshCell Communication - Physiologyen_US
dc.subject.meshCell Lineen_US
dc.subject.meshCilia - Metabolism - Physiologyen_US
dc.subject.meshEpithelium - Metabolism - Physiologyen_US
dc.subject.meshFallopian Tubes - Metabolismen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation - Drug Effectsen_US
dc.subject.meshHormones - Metabolism - Pharmacologyen_US
dc.subject.meshHumansen_US
dc.subject.meshIntracellular Signaling Peptides And Proteins - Genetics - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMembrane Proteins - Genetics - Metabolismen_US
dc.subject.meshMenstrual Cycle - Genetics - Metabolism - Physiologyen_US
dc.subject.meshModels, Biologicalen_US
dc.subject.meshReceptor Activity-Modifying Proteinsen_US
dc.subject.meshReceptors, Calcitonin - Genetics - Metabolismen_US
dc.subject.meshSpermatozoa - Metabolism - Physiologyen_US
dc.titleExpression of adrenomedullin in human oviduct, its regulation by the hormonal cycle and contact with spermatozoa, and its effect on ciliary beat frequency of the oviductal epitheliumen_HK
dc.typeArticleen_HK
dc.identifier.emailChiu, PCN: pchiucn@hku.hken_HK
dc.identifier.emailHo, JC: jhocm@hku.hken_HK
dc.identifier.emailNg, EHY: nghye@hkucc.hku.hken_HK
dc.identifier.emailHo, PC: pcho@hku.hken_HK
dc.identifier.emailYeung, WSB: wsbyeung@hkucc.hku.hken_HK
dc.identifier.emailTang, F: ftang@hkucc.hku.hken_HK
dc.identifier.emailO, WS: owaisum@hkucc.hku.hken_HK
dc.identifier.authorityChiu, PCN=rp00424en_HK
dc.identifier.authorityHo, JC=rp00258en_HK
dc.identifier.authorityNg, EHY=rp00426en_HK
dc.identifier.authorityHo, PC=rp00325en_HK
dc.identifier.authorityYeung, WSB=rp00331en_HK
dc.identifier.authorityTang, F=rp00327en_HK
dc.identifier.authorityO, WS=rp00315en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1210/jc.2010-0273en_HK
dc.identifier.pmid20534761-
dc.identifier.scopuseid_2-s2.0-77956584679en_HK
dc.identifier.hkuros182871-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956584679&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume95en_HK
dc.identifier.issue9en_HK
dc.identifier.spageE18en_HK
dc.identifier.epageE25en_HK
dc.identifier.eissn1945-7197-
dc.identifier.isiWOS:000281640300003-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLi, HWR=36652816700en_HK
dc.identifier.scopusauthoridLiao, SB=36170881600en_HK
dc.identifier.scopusauthoridChiu, PCN=25959969200en_HK
dc.identifier.scopusauthoridTam, WW=36653271400en_HK
dc.identifier.scopusauthoridHo, JC=7402649981en_HK
dc.identifier.scopusauthoridNg, EHY=35238184300en_HK
dc.identifier.scopusauthoridHo, PC=7402211440en_HK
dc.identifier.scopusauthoridYeung, WSB=7102370745en_HK
dc.identifier.scopusauthoridTang, F=7201979770en_HK
dc.identifier.scopusauthoridO, WS=6701729369en_HK

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