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Article: Clinical correlation of nuclear survivin in esophageal squamous cell carcinoma

TitleClinical correlation of nuclear survivin in esophageal squamous cell carcinoma
Authors
KeywordsBiomarker
Esophageal squamous cell carcinoma
Nodal metastasis
Nuclear survivin
Pathological stage
Issue Date2012
PublisherHumana Press, Inc. The Journal's web site is located at http://www.humanapress.com/JournalDetail.pasp?journal=14
Citation
Medical Oncology, 2012, v. 29 n. 5, p. 3009-3016 How to Cite?
AbstractTo examine the correlation of survivin (both total and nuclear survivin) with clinicopathological parameters of esophageal squamous cell carcinoma (ESCC) patients. Tumors and non-tumor tissues near the proximal resection margins were resected from ESCC patients undergone esophagectomy. Quantitative polymerase chain reaction (qPCR) was performed to detect survivin mRNA expression level in the 10 paired tumor and adjacent non-tumor tissues. To confirm with the clinical situation, survivin mRNA and protein expression were measured by qPCR and immunoblot, respectively, in 5 ESCC cell lines and a non-neoplastic esophageal epithelial cell line. Immunohistochemistry was employed to reveal the cellular localization of survivin in tumor tissues isolated from the 64 ESCC patients undergone surgery alone. Up-regulation of survivin mRNA and protein was found in 5 ESCC lines (HKESC-1, HKESC-2, HKESC-3, HKESC-4, and SLMT-1) when compared to a non-neoplastic esophageal epithelial cell line NE-1. In particular, HKESC-3, HKESC-4, and SLMT-1 cells demonstrated ~50-fold increase in survivin mRNA. High level of survivin mRNA in tumor tissues when compared to non-tumor tissues was found in 70 % (7 of 10) of clinical cases. The increase in expression ranged from ~twofold to ~16-fold. Immunohistochemistry results showed that survivin was found at the cell nuclei in all specimens examined. Nuclear expression of survivin was inversely associated with the likelihood of developing nodal metastasis (p = 0.021) and significantly associated with early-stage ESCC (p = 0.039). Nuclear survivin could serve as a marker for indicating disease status in ESCC patients. © 2012 The Author(s).
Persistent Identifierhttp://hdl.handle.net/10722/148693
ISSN
2015 Impact Factor: 2.486
2015 SCImago Journal Rankings: 0.837
PubMed Central ID
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorHui, MKCen_HK
dc.contributor.authorLai, KKYen_HK
dc.contributor.authorChan, KWen_HK
dc.contributor.authorLuk, JMen_HK
dc.contributor.authorLee, NPen_HK
dc.contributor.authorChung, Yen_HK
dc.contributor.authorCheung, LCMen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorTang, JCen_HK
dc.contributor.authorLaw, Sen_HK
dc.date.accessioned2012-05-29T06:14:43Z-
dc.date.available2012-05-29T06:14:43Z-
dc.date.issued2012en_HK
dc.identifier.citationMedical Oncology, 2012, v. 29 n. 5, p. 3009-3016en_HK
dc.identifier.issn1357-0560en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148693-
dc.description.abstractTo examine the correlation of survivin (both total and nuclear survivin) with clinicopathological parameters of esophageal squamous cell carcinoma (ESCC) patients. Tumors and non-tumor tissues near the proximal resection margins were resected from ESCC patients undergone esophagectomy. Quantitative polymerase chain reaction (qPCR) was performed to detect survivin mRNA expression level in the 10 paired tumor and adjacent non-tumor tissues. To confirm with the clinical situation, survivin mRNA and protein expression were measured by qPCR and immunoblot, respectively, in 5 ESCC cell lines and a non-neoplastic esophageal epithelial cell line. Immunohistochemistry was employed to reveal the cellular localization of survivin in tumor tissues isolated from the 64 ESCC patients undergone surgery alone. Up-regulation of survivin mRNA and protein was found in 5 ESCC lines (HKESC-1, HKESC-2, HKESC-3, HKESC-4, and SLMT-1) when compared to a non-neoplastic esophageal epithelial cell line NE-1. In particular, HKESC-3, HKESC-4, and SLMT-1 cells demonstrated ~50-fold increase in survivin mRNA. High level of survivin mRNA in tumor tissues when compared to non-tumor tissues was found in 70 % (7 of 10) of clinical cases. The increase in expression ranged from ~twofold to ~16-fold. Immunohistochemistry results showed that survivin was found at the cell nuclei in all specimens examined. Nuclear expression of survivin was inversely associated with the likelihood of developing nodal metastasis (p = 0.021) and significantly associated with early-stage ESCC (p = 0.039). Nuclear survivin could serve as a marker for indicating disease status in ESCC patients. © 2012 The Author(s).en_HK
dc.languageengen_US
dc.publisherHumana Press, Inc. The Journal's web site is located at http://www.humanapress.com/JournalDetail.pasp?journal=14en_HK
dc.relation.ispartofMedical Oncologyen_HK
dc.rightsThe original publication is available at www.springerlink.com-
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subjectBiomarkeren_HK
dc.subjectEsophageal squamous cell carcinomaen_HK
dc.subjectNodal metastasisen_HK
dc.subjectNuclear survivinen_HK
dc.subjectPathological stageen_HK
dc.titleClinical correlation of nuclear survivin in esophageal squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailChan, KW: hrmtckw@hku.hken_HK
dc.identifier.emailLuk, JM: jmluk@hkucc.hku.hken_HK
dc.identifier.emailLee, NP: nikkilee@hku.hken_HK
dc.identifier.emailSrivastava, G: gopesh@pathology.hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hkucc.hku.hken_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.authorityChan, KW=rp00330en_HK
dc.identifier.authorityLuk, JM=rp00349en_HK
dc.identifier.authorityLee, NP=rp00263en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1007/s12032-012-0225-9en_HK
dc.identifier.pmid22528514-
dc.identifier.pmcidPMC3505527-
dc.identifier.scopuseid_2-s2.0-84880298437en_HK
dc.identifier.hkuros208597-
dc.identifier.hkuros212757-
dc.identifier.spage3009en_HK
dc.identifier.epage3016en_HK
dc.identifier.isiWOS:000311513800001-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridHui, MKC=8644138500en_HK
dc.identifier.scopusauthoridLai, KKY=54400333100en_HK
dc.identifier.scopusauthoridChan, KW=16444133100en_HK
dc.identifier.scopusauthoridLuk, JM=7006777791en_HK
dc.identifier.scopusauthoridLee, NP=7402722690en_HK
dc.identifier.scopusauthoridChung, Y=22833625500en_HK
dc.identifier.scopusauthoridCheung, LCM=21740536900en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridTang, JC=14056850300en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.citeulike10632044-

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