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Article: Enhanced HMGB1 expression may contribute to Th17 cells activation in rheumatoid arthritis

TitleEnhanced HMGB1 expression may contribute to Th17 cells activation in rheumatoid arthritis
Authors
Issue Date2012
PublisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/cdi/
Citation
Clinical & Developmental Immunology, 2012, v. 2012, article no. 295081 How to Cite?
Abstract
Rheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORgammat, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORgammat, and IL-17 in patients were higher than that in health control (P < 0.05), especially in active RA patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P < 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients.
Persistent Identifierhttp://hdl.handle.net/10722/148675
ISSN
2013 Impact Factor: 2.934
PubMed Central ID
ISI Accession Number ID
References

 

Author Affiliations
  1. Jiangsu University
  2. Suzhou Municipal Hospital
DC FieldValueLanguage
dc.contributor.authorShi, Yen_US
dc.contributor.authorSandoghchian Shotorbani, Sen_US
dc.contributor.authorSu, Zen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorTong, Jen_US
dc.contributor.authorZheng, Den_US
dc.contributor.authorChen, Jen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorXu, Yen_US
dc.contributor.authorJiao, Zen_US
dc.contributor.authorWang, Sen_US
dc.contributor.authorLu, Len_US
dc.contributor.authorHuang, Xen_US
dc.contributor.authorXu, Hen_US
dc.date.accessioned2012-05-29T06:14:36Z-
dc.date.available2012-05-29T06:14:36Z-
dc.date.issued2012en_US
dc.identifier.citationClinical & Developmental Immunology, 2012, v. 2012, article no. 295081en_US
dc.identifier.issn1740-2522en_US
dc.identifier.urihttp://hdl.handle.net/10722/148675-
dc.description.abstractRheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORgammat, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORgammat, and IL-17 in patients were higher than that in health control (P < 0.05), especially in active RA patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P < 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients.en_US
dc.languageengen_US
dc.publisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/cdi/en_US
dc.relation.ispartofClinical & Developmental Immunologyen_US
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License-
dc.subject.meshArthritis, Rheumatoid - genetics - immunology - metabolism-
dc.subject.meshCD4-Positive T-Lymphocytes - immunology-
dc.subject.meshHMGB1 Protein - genetics - metabolism-
dc.subject.meshLymphocyte Activation - immunology-
dc.subject.meshTh17 Cells - immunology-
dc.titleEnhanced HMGB1 expression may contribute to Th17 cells activation in rheumatoid arthritisen_US
dc.typeArticleen_US
dc.identifier.emailSu, Z: szl30@yeah.neten_US
dc.identifier.emailLu, L: liweilu@hkucc.hku.hk-
dc.identifier.emailXu, H: xuhx@ujs.edu.cn-
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturepublished_or_final_versionen_US
dc.identifier.doi10.1155/2012/295081en_US
dc.identifier.pmid22110531-
dc.identifier.pmcidPMC3205666-
dc.identifier.scopuseid_2-s2.0-84855173538en_US
dc.identifier.hkuros208181-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-84855173538&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume2012, article no. 295081en_US
dc.identifier.isiWOS:000308591700001-
dc.publisher.placeUnited Statesen_US

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