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Article: Enhanced HMGB1 expression may contribute to Th17 cells activation in rheumatoid arthritis
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TitleEnhanced HMGB1 expression may contribute to Th17 cells activation in rheumatoid arthritis
 
AuthorsShi, Y1 2
Sandoghchian Shotorbani, S1
Su, Z1
Liu, Y1
Tong, J1
Zheng, D1
Chen, J1
Liu, Y1
Xu, Y1
Jiao, Z1
Wang, S1
Lu, L1
Huang, X1
Xu, H1
 
Issue Date2012
 
PublisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/cdi/
 
CitationClinical & Developmental Immunology, 2012, v. 2012, article no. 295081 [How to Cite?]
DOI: http://dx.doi.org/10.1155/2012/295081
 
AbstractRheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORgammat, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORgammat, and IL-17 in patients were higher than that in health control (P < 0.05), especially in active RA patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P < 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients.
 
ISSN1740-2522
2013 Impact Factor: 2.934
 
DOIhttp://dx.doi.org/10.1155/2012/295081
 
PubMed Central IDPMC3205666
 
ISI Accession Number IDWOS:000308591700001
 
ReferencesReferences in Scopus
 
DC FieldValue
dc.contributor.authorShi, Y
 
dc.contributor.authorSandoghchian Shotorbani, S
 
dc.contributor.authorSu, Z
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorTong, J
 
dc.contributor.authorZheng, D
 
dc.contributor.authorChen, J
 
dc.contributor.authorLiu, Y
 
dc.contributor.authorXu, Y
 
dc.contributor.authorJiao, Z
 
dc.contributor.authorWang, S
 
dc.contributor.authorLu, L
 
dc.contributor.authorHuang, X
 
dc.contributor.authorXu, H
 
dc.date.accessioned2012-05-29T06:14:36Z
 
dc.date.available2012-05-29T06:14:36Z
 
dc.date.issued2012
 
dc.description.abstractRheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORgammat, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORgammat, and IL-17 in patients were higher than that in health control (P < 0.05), especially in active RA patients (P < 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P < 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients.
 
dc.description.naturepublished_or_final_version
 
dc.identifier.citationClinical & Developmental Immunology, 2012, v. 2012, article no. 295081 [How to Cite?]
DOI: http://dx.doi.org/10.1155/2012/295081
 
dc.identifier.doihttp://dx.doi.org/10.1155/2012/295081
 
dc.identifier.hkuros208181
 
dc.identifier.isiWOS:000308591700001
 
dc.identifier.issn1740-2522
2013 Impact Factor: 2.934
 
dc.identifier.pmcidPMC3205666
 
dc.identifier.pmid22110531
 
dc.identifier.scopuseid_2-s2.0-84855173538
 
dc.identifier.urihttp://hdl.handle.net/10722/148675
 
dc.identifier.volume2012, article no. 295081
 
dc.languageeng
 
dc.publisherHindawi Publishing Corporation. The Journal's web site is located at http://www.hindawi.com/journals/cdi/
 
dc.publisher.placeUnited States
 
dc.relation.ispartofClinical & Developmental Immunology
 
dc.relation.referencesReferences in Scopus
 
dc.rightsCreative Commons: Attribution 3.0 Hong Kong License
 
dc.subject.meshArthritis, Rheumatoid - genetics - immunology - metabolism
 
dc.subject.meshCD4-Positive T-Lymphocytes - immunology
 
dc.subject.meshHMGB1 Protein - genetics - metabolism
 
dc.subject.meshLymphocyte Activation - immunology
 
dc.subject.meshTh17 Cells - immunology
 
dc.titleEnhanced HMGB1 expression may contribute to Th17 cells activation in rheumatoid arthritis
 
dc.typeArticle
 
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<contributor.author>Tong, J</contributor.author>
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<contributor.author>Chen, J</contributor.author>
<contributor.author>Liu, Y</contributor.author>
<contributor.author>Xu, Y</contributor.author>
<contributor.author>Jiao, Z</contributor.author>
<contributor.author>Wang, S</contributor.author>
<contributor.author>Lu, L</contributor.author>
<contributor.author>Huang, X</contributor.author>
<contributor.author>Xu, H</contributor.author>
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<description.abstract>Rheumatoid arthritis(RA) is a common autoimmune disease associated with Th17 cells, but what about the effect of high-mobility group box chromosomal protein 1 (HMGB1) and the relationship between Th17-associated factors and HMGB1 in RA remains unknown. In the present study, we investigated the mRNA levels of HMGB1, RORgammat, and IL-17 in peripheral blood mononuclear cells (PBMCs) from patients with rheumatoid arthritis by quantitative real-time PCR (RT-qPCR), and the concentrations of HMGB1, IL-17, and IL-23 in plasma were detected by ELISA. And then, the effect of HMGB1 on Th17 cells differentiation was analyzed in vitro. Our clinical studies showed that the mRNAs of HMGB1, RORgammat, and IL-17 in patients were higher than that in health control (P &lt; 0.05), especially in active RA patients (P &lt; 0.05). The plasma HMGB1, IL-17, and IL-23 in RA patients were also higher than that in health control (P &lt; 0.05); there was a positive correlation between the expression levels of HMGB1 and the amount of CRP, ERS, and RF in plasma. In vitro, the IL-17-produced CD4(+)T cells were increased with 100 ng/mL rHMGB1 for 12h, which indicated that the increased HMGB1 might contribute to Th17 cells activation in RA patients.</description.abstract>
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Author Affiliations
  1. Jiangsu University
  2. Suzhou Municipal Hospital