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Article: Overexpression of the parkinson disease protein DJ-1 and its regulator PTEN in gestational trophoblastic disease

TitleOverexpression of the parkinson disease protein DJ-1 and its regulator PTEN in gestational trophoblastic disease
Authors
KeywordsDJ-1
Gestational trophoblastic disease
PTEN
Issue Date2010
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.intjgynpathology.com
Citation
International Journal Of Gynecological Pathology, 2010, v. 29 n. 5, p. 468-475 How to Cite?
AbstractDJ-1 is found to be important in human neurodegenerative diseases and cancers by regulating oxidative damage and apoptosis. DJ-1 is also a regulator of PTEN, a frequently mutated tumor suppressor gene in cancers. In this study, we investigated the expression of DJ-1 and PTEN in normal placentas and gestational trophoblastic disease in relation to apoptotic indices and p53 status. A total of 95 trophoblastic samples were retrieved for immunohistochemical study whereas 79 trophoblastic samples, 3 normal trophoblastic and 2 choriocarcinoma cell lines were collected for quantitative real time reverse transcription polymerase chain reaction detection. There was a significant correlation between DJ-1 and PTEN immunostaining indices in the trophoblastic samples (P=0.013). Significantly higher DJ-1 and PTEN immunoreactivity indices were found in the complete mole (P<0.01) and choricarcinoma (P<0.01) compared with the first trimester placenta. Quantitative real time reverse transcription polymerase chain reaction also detected significantly higher messenger ribonucleic acid expressions of DJ-1 and PTEN in hydatidiform moles (P<0.05) and choriocarcinomas (P<0.05) compared with the first trimester placentas. A significant negative correlation was found between DJ-1 and the apoptosis resistant gene Bcl-2 (P=0.031), whereas a positive correlation was shown between PTEN and wild-type p53 (P=0.019). Significant correlations between PTEN and embryonic stem cell transcription factors, Stat3 and Nanog, were also displayed (P=0.001, 0.015). Our findings showed, for the first time, overexpression of DJ-1 at both transcriptional and protein levels in gestational trophoblastic disease. Overexpressed DJ-1 may play a role in regulating apoptotic activities of trophoblasts in relation to PTEN and p53. © 2010 International Society of Gynecological Pathologists.
Persistent Identifierhttp://hdl.handle.net/10722/148628
ISSN
2015 Impact Factor: 1.437
2015 SCImago Journal Rankings: 0.723
ISI Accession Number ID
Funding AgencyGrant Number
Hong Kong Research Grant CouncilHKU 7503/06M
University of Hong Kong Committee
Funding Information:

Supported by research grants from the Hong Kong Research Grant Council Grant (HKU 7503/06M) and the University of Hong Kong Committee for Research and Conference Grant.

References
Grants

 

DC FieldValueLanguage
dc.contributor.authorZhang, HJen_HK
dc.contributor.authorSiu, MKYen_HK
dc.contributor.authorJiang, LLen_HK
dc.contributor.authorMak, VCYen_HK
dc.contributor.authorNgan, HYSen_HK
dc.contributor.authorCheung, ANYen_HK
dc.date.accessioned2012-05-29T06:14:12Z-
dc.date.available2012-05-29T06:14:12Z-
dc.date.issued2010en_HK
dc.identifier.citationInternational Journal Of Gynecological Pathology, 2010, v. 29 n. 5, p. 468-475en_HK
dc.identifier.issn0277-1691en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148628-
dc.description.abstractDJ-1 is found to be important in human neurodegenerative diseases and cancers by regulating oxidative damage and apoptosis. DJ-1 is also a regulator of PTEN, a frequently mutated tumor suppressor gene in cancers. In this study, we investigated the expression of DJ-1 and PTEN in normal placentas and gestational trophoblastic disease in relation to apoptotic indices and p53 status. A total of 95 trophoblastic samples were retrieved for immunohistochemical study whereas 79 trophoblastic samples, 3 normal trophoblastic and 2 choriocarcinoma cell lines were collected for quantitative real time reverse transcription polymerase chain reaction detection. There was a significant correlation between DJ-1 and PTEN immunostaining indices in the trophoblastic samples (P=0.013). Significantly higher DJ-1 and PTEN immunoreactivity indices were found in the complete mole (P<0.01) and choricarcinoma (P<0.01) compared with the first trimester placenta. Quantitative real time reverse transcription polymerase chain reaction also detected significantly higher messenger ribonucleic acid expressions of DJ-1 and PTEN in hydatidiform moles (P<0.05) and choriocarcinomas (P<0.05) compared with the first trimester placentas. A significant negative correlation was found between DJ-1 and the apoptosis resistant gene Bcl-2 (P=0.031), whereas a positive correlation was shown between PTEN and wild-type p53 (P=0.019). Significant correlations between PTEN and embryonic stem cell transcription factors, Stat3 and Nanog, were also displayed (P=0.001, 0.015). Our findings showed, for the first time, overexpression of DJ-1 at both transcriptional and protein levels in gestational trophoblastic disease. Overexpressed DJ-1 may play a role in regulating apoptotic activities of trophoblasts in relation to PTEN and p53. © 2010 International Society of Gynecological Pathologists.en_HK
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.intjgynpathology.comen_HK
dc.relation.ispartofInternational Journal of Gynecological Pathologyen_HK
dc.subjectDJ-1en_HK
dc.subjectGestational trophoblastic diseaseen_HK
dc.subjectPTENen_HK
dc.subject.meshApoptosis - Physiologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshGestational Trophoblastic Disease - Genetics - Metabolism - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIntracellular Signaling Peptides And Proteins - Geneticsen_US
dc.subject.meshOncogene Proteins - Biosynthesis - Geneticsen_US
dc.subject.meshPten Phosphohydrolase - Biosynthesis - Geneticsen_US
dc.subject.meshPlacenta - Metabolism - Pathologyen_US
dc.subject.meshPregnancyen_US
dc.subject.meshReverse Transcriptase Polymerase Chain Reactionen_US
dc.subject.meshUterine Neoplasms - Genetics - Metabolism - Pathologyen_US
dc.titleOverexpression of the parkinson disease protein DJ-1 and its regulator PTEN in gestational trophoblastic diseaseen_HK
dc.typeArticleen_HK
dc.identifier.emailSiu, MKY: mkysiu@hkucc.hku.hken_HK
dc.identifier.emailNgan, HYS: hysngan@hkucc.hku.hken_HK
dc.identifier.emailCheung, ANY: anycheun@hkucc.hku.hken_HK
dc.identifier.authoritySiu, MKY=rp00275en_HK
dc.identifier.authorityNgan, HYS=rp00346en_HK
dc.identifier.authorityCheung, ANY=rp00542en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/PGP.0b013e3181de3068en_HK
dc.identifier.pmid20736773-
dc.identifier.scopuseid_2-s2.0-77956224325en_HK
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77956224325&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume29en_HK
dc.identifier.issue5en_HK
dc.identifier.spage468en_HK
dc.identifier.epage475en_HK
dc.identifier.isiWOS:000281521600012-
dc.publisher.placeUnited Statesen_HK
dc.relation.projectAkt and p21-activated kinase signaling pathways in gestational trophoblastic disease-
dc.identifier.scopusauthoridZhang, HJ=8597830900en_HK
dc.identifier.scopusauthoridSiu, MKY=24924018400en_HK
dc.identifier.scopusauthoridJiang, LL=36801738800en_HK
dc.identifier.scopusauthoridMak, VCY=36508943200en_HK
dc.identifier.scopusauthoridNgan, HYS=34571944100en_HK
dc.identifier.scopusauthoridCheung, ANY=54927484100en_HK

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