Article: Differentiation imbalance of Th1/Th17 in peripheral blood mononuclear cells might contribute to pathogenesis of Hashimoto's thyroiditis

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TitleDifferentiation imbalance of Th1/Th17 in peripheral blood mononuclear cells might contribute to pathogenesis of Hashimoto's thyroiditis
AuthorsShi, Y1
Wang, H1
Su, Z1
Chen, J1
Xue, Y1
Wang, S1
Xue, Y1
He, Z1
Yang, H1
Zhou, C1
Kong, F1
Liu, Y1
Yang, P1
Lu, L2
Shao, Q1
Huang, X1
Xu, H1
Issue Date2010
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/SJI
CitationScandinavian Journal Of Immunology, 2010, v. 72 n. 3, p. 250-255 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1365-3083.2010.02425.x
AbstractT helper 17(Th17) cell is a new subset of CD4+ T cells that produce a proinflammatory cytokine interleukin-17 (IL-17). Th17 cells have recently been shown to play a critical role in many autoimmune diseases that had previously been thought to be Th1 dominant. Although Hashimoto's thyroiditis (HT) was thought to be a Th1-type disease, the contributions of Th17 cells to the pathogenesis remain unclear. In this study, we investigated the expression levels of Th1/Th17 cell-associated factors in peripheral blood mononuclear cells (PBMC) and plasma from patients with HT by quantitative real-time polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). Our results showed that the expression levels of Th1 cells-related T-bet and interferon-γ (IFN-γ) mRNA in PBMC from HT significantly decreased. However, the mRNA of Th17 coherent retinoic acid-related orphan nuclear receptor gamma t (RORγt) and IL-17 in patients with HT increased. In addition, a negative correlation between T-bet and RORγt mRNA expression was found in patients with HT, and the similar phenomena also appeared on the levels of mRNA and plasma concentration between IFN-γ and IL-17. It suggested that Th17 cells rather than Th1 cells predominated among patients suffering from HT, and Th17 cells might be involved in the pathogenesis of HT. © 2010 Blackwell Publishing Ltd.
ISSN0300-9475
2011 Impact Factor: 2.23
2011 SCImago Journal Rankings: 0.229
DOIhttp://dx.doi.org/10.1111/j.1365-3083.2010.02425.x
ReferencesReferences in Scopus
DC Field
Value
dc.contributor.authorShi, Y
dc.contributor.authorWang, H
dc.contributor.authorSu, Z
dc.contributor.authorChen, J
dc.contributor.authorXue, Y
dc.contributor.authorWang, S
dc.contributor.authorXue, Y
dc.contributor.authorHe, Z
dc.contributor.authorYang, H
dc.contributor.authorZhou, C
dc.contributor.authorKong, F
dc.contributor.authorLiu, Y
dc.contributor.authorYang, P
dc.contributor.authorLu, L
dc.contributor.authorShao, Q
dc.contributor.authorHuang, X
dc.contributor.authorXu, H
dc.date.accessioned2012-05-29T06:14:12Z
dc.date.available2012-05-29T06:14:12Z
dc.date.issued2010
dc.description.abstractT helper 17(Th17) cell is a new subset of CD4+ T cells that produce a proinflammatory cytokine interleukin-17 (IL-17). Th17 cells have recently been shown to play a critical role in many autoimmune diseases that had previously been thought to be Th1 dominant. Although Hashimoto's thyroiditis (HT) was thought to be a Th1-type disease, the contributions of Th17 cells to the pathogenesis remain unclear. In this study, we investigated the expression levels of Th1/Th17 cell-associated factors in peripheral blood mononuclear cells (PBMC) and plasma from patients with HT by quantitative real-time polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). Our results showed that the expression levels of Th1 cells-related T-bet and interferon-γ (IFN-γ) mRNA in PBMC from HT significantly decreased. However, the mRNA of Th17 coherent retinoic acid-related orphan nuclear receptor gamma t (RORγt) and IL-17 in patients with HT increased. In addition, a negative correlation between T-bet and RORγt mRNA expression was found in patients with HT, and the similar phenomena also appeared on the levels of mRNA and plasma concentration between IFN-γ and IL-17. It suggested that Th17 cells rather than Th1 cells predominated among patients suffering from HT, and Th17 cells might be involved in the pathogenesis of HT. © 2010 Blackwell Publishing Ltd.
dc.description.natureLink_to_subscribed_fulltext
dc.identifier.citationScandinavian Journal Of Immunology, 2010, v. 72 n. 3, p. 250-255 [How to Cite?]
DOI: http://dx.doi.org/10.1111/j.1365-3083.2010.02425.x
dc.identifier.citeulike7616476
dc.identifier.doihttp://dx.doi.org/10.1111/j.1365-3083.2010.02425.x
dc.identifier.epage255
dc.identifier.hkuros176946
dc.identifier.isiWOS:000280638800012
Funding AgencyGrant Number
National Natural Science Foundation of China30871193
30972748
Natural Science Foundation of Jiangsu Province Educational Commission09KJB310001
Innovation Fund for candidate of doctor in Jiangsu ProvinceCX09B_217Z
Health Department Foundation of Jiangsu ProvinceH200952
Funding Information:

This work was supported by the National Natural Science Foundation of China (Grant NO. 30871193, 30972748, respectively); Natural Science Foundation of Jiangsu Province Educational Commission (Grant No. 09KJB310001); Innovation Fund for candidate of doctor in Jiangsu Province (Grant NO. CX09B_217Z); Health Department Foundation of Jiangsu Province (Grant NO.H200952).

dc.identifier.issn0300-9475
2011 Impact Factor: 2.23
2011 SCImago Journal Rankings: 0.229
dc.identifier.issue3
dc.identifier.pmid20696023
dc.identifier.scopuseid_2-s2.0-77955298749
dc.identifier.spage250
dc.identifier.urihttp://hdl.handle.net/10722/148626
dc.identifier.volume72
dc.languageeng
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/SJI
dc.publisher.placeUnited Kingdom
dc.relation.ispartofScandinavian Journal of Immunology
dc.relation.referencesReferences in Scopus
dc.subject.meshAdult
dc.subject.meshCell Differentiation - Immunology
dc.subject.meshFemale
dc.subject.meshGene Expression - Genetics
dc.subject.meshHashimoto Disease - Blood - Etiology - Immunology - Pathology
dc.subject.meshHumans
dc.subject.meshInterferon-Gamma - Blood - Genetics
dc.subject.meshInterleukin-17 - Blood - Genetics - Metabolism
dc.subject.meshInterleukin-23 Subunit P19 - Blood - Genetics
dc.subject.meshInterleukin-6 - Blood - Genetics
dc.subject.meshLeukocytes, Mononuclear - Cytology - Immunology - Metabolism
dc.subject.meshMale
dc.subject.meshMiddle Aged
dc.subject.meshNuclear Receptor Subfamily 1, Group F, Member 3 - Genetics
dc.subject.meshT-Box Domain Proteins - Genetics
dc.subject.meshT-Lymphocytes, Helper-Inducer - Cytology - Immunology - Metabolism - Pathology
dc.subject.meshTh1 Cells - Cytology - Immunology - Metabolism - Pathology
dc.subject.meshYoung Adult
dc.titleDifferentiation imbalance of Th1/Th17 in peripheral blood mononuclear cells might contribute to pathogenesis of Hashimoto's thyroiditis
dc.typeArticle
Author Affiliations
  1. Jiangsu University
  2. The University of Hong Kong