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Article: Differentiation imbalance of Th1/Th17 in peripheral blood mononuclear cells might contribute to pathogenesis of Hashimoto's thyroiditis

TitleDifferentiation imbalance of Th1/Th17 in peripheral blood mononuclear cells might contribute to pathogenesis of Hashimoto's thyroiditis
Authors
Issue Date2010
PublisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/SJI
Citation
Scandinavian Journal Of Immunology, 2010, v. 72 n. 3, p. 250-255 How to Cite?
AbstractT helper 17(Th17) cell is a new subset of CD4+ T cells that produce a proinflammatory cytokine interleukin-17 (IL-17). Th17 cells have recently been shown to play a critical role in many autoimmune diseases that had previously been thought to be Th1 dominant. Although Hashimoto's thyroiditis (HT) was thought to be a Th1-type disease, the contributions of Th17 cells to the pathogenesis remain unclear. In this study, we investigated the expression levels of Th1/Th17 cell-associated factors in peripheral blood mononuclear cells (PBMC) and plasma from patients with HT by quantitative real-time polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). Our results showed that the expression levels of Th1 cells-related T-bet and interferon-γ (IFN-γ) mRNA in PBMC from HT significantly decreased. However, the mRNA of Th17 coherent retinoic acid-related orphan nuclear receptor gamma t (RORγt) and IL-17 in patients with HT increased. In addition, a negative correlation between T-bet and RORγt mRNA expression was found in patients with HT, and the similar phenomena also appeared on the levels of mRNA and plasma concentration between IFN-γ and IL-17. It suggested that Th17 cells rather than Th1 cells predominated among patients suffering from HT, and Th17 cells might be involved in the pathogenesis of HT. © 2010 Blackwell Publishing Ltd.
Persistent Identifierhttp://hdl.handle.net/10722/148626
ISSN
2014 Impact Factor: 1.739
2014 SCImago Journal Rankings: 0.746
ISI Accession Number ID
Funding AgencyGrant Number
National Natural Science Foundation of China30871193
30972748
Natural Science Foundation of Jiangsu Province Educational Commission09KJB310001
Innovation Fund for candidate of doctor in Jiangsu ProvinceCX09B_217Z
Health Department Foundation of Jiangsu ProvinceH200952
Funding Information:

This work was supported by the National Natural Science Foundation of China (Grant NO. 30871193, 30972748, respectively); Natural Science Foundation of Jiangsu Province Educational Commission (Grant No. 09KJB310001); Innovation Fund for candidate of doctor in Jiangsu Province (Grant NO. CX09B_217Z); Health Department Foundation of Jiangsu Province (Grant NO.H200952).

References

 

DC FieldValueLanguage
dc.contributor.authorShi, Yen_US
dc.contributor.authorWang, Hen_US
dc.contributor.authorSu, Zen_US
dc.contributor.authorChen, Jen_US
dc.contributor.authorXue, Yen_US
dc.contributor.authorWang, Sen_US
dc.contributor.authorXue, Yen_US
dc.contributor.authorHe, Zen_US
dc.contributor.authorYang, Hen_US
dc.contributor.authorZhou, Cen_US
dc.contributor.authorKong, Fen_US
dc.contributor.authorLiu, Yen_US
dc.contributor.authorYang, Pen_US
dc.contributor.authorLu, Len_US
dc.contributor.authorShao, Qen_US
dc.contributor.authorHuang, Xen_US
dc.contributor.authorXu, Hen_US
dc.date.accessioned2012-05-29T06:14:12Z-
dc.date.available2012-05-29T06:14:12Z-
dc.date.issued2010en_US
dc.identifier.citationScandinavian Journal Of Immunology, 2010, v. 72 n. 3, p. 250-255en_US
dc.identifier.issn0300-9475en_US
dc.identifier.urihttp://hdl.handle.net/10722/148626-
dc.description.abstractT helper 17(Th17) cell is a new subset of CD4+ T cells that produce a proinflammatory cytokine interleukin-17 (IL-17). Th17 cells have recently been shown to play a critical role in many autoimmune diseases that had previously been thought to be Th1 dominant. Although Hashimoto's thyroiditis (HT) was thought to be a Th1-type disease, the contributions of Th17 cells to the pathogenesis remain unclear. In this study, we investigated the expression levels of Th1/Th17 cell-associated factors in peripheral blood mononuclear cells (PBMC) and plasma from patients with HT by quantitative real-time polymerase chain reaction (RT-qPCR) and enzyme-linked immunosorbent assay (ELISA). Our results showed that the expression levels of Th1 cells-related T-bet and interferon-γ (IFN-γ) mRNA in PBMC from HT significantly decreased. However, the mRNA of Th17 coherent retinoic acid-related orphan nuclear receptor gamma t (RORγt) and IL-17 in patients with HT increased. In addition, a negative correlation between T-bet and RORγt mRNA expression was found in patients with HT, and the similar phenomena also appeared on the levels of mRNA and plasma concentration between IFN-γ and IL-17. It suggested that Th17 cells rather than Th1 cells predominated among patients suffering from HT, and Th17 cells might be involved in the pathogenesis of HT. © 2010 Blackwell Publishing Ltd.en_US
dc.languageengen_US
dc.publisherBlackwell Publishing Ltd. The Journal's web site is located at http://www.blackwellpublishing.com/journals/SJIen_US
dc.relation.ispartofScandinavian Journal of Immunologyen_US
dc.subject.meshAdulten_US
dc.subject.meshCell Differentiation - Immunologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression - Geneticsen_US
dc.subject.meshHashimoto Disease - Blood - Etiology - Immunology - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshInterferon-Gamma - Blood - Geneticsen_US
dc.subject.meshInterleukin-17 - Blood - Genetics - Metabolismen_US
dc.subject.meshInterleukin-23 Subunit P19 - Blood - Geneticsen_US
dc.subject.meshInterleukin-6 - Blood - Geneticsen_US
dc.subject.meshLeukocytes, Mononuclear - Cytology - Immunology - Metabolismen_US
dc.subject.meshMaleen_US
dc.subject.meshMiddle Ageden_US
dc.subject.meshNuclear Receptor Subfamily 1, Group F, Member 3 - Geneticsen_US
dc.subject.meshT-Box Domain Proteins - Geneticsen_US
dc.subject.meshT-Lymphocytes, Helper-Inducer - Cytology - Immunology - Metabolism - Pathologyen_US
dc.subject.meshTh1 Cells - Cytology - Immunology - Metabolism - Pathologyen_US
dc.subject.meshYoung Adulten_US
dc.titleDifferentiation imbalance of Th1/Th17 in peripheral blood mononuclear cells might contribute to pathogenesis of Hashimoto's thyroiditisen_US
dc.typeArticleen_US
dc.identifier.emailLu, L:liweilu@hkucc.hku.hken_US
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1111/j.1365-3083.2010.02425.xen_US
dc.identifier.pmid20696023-
dc.identifier.scopuseid_2-s2.0-77955298749en_US
dc.identifier.hkuros176946-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-77955298749&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume72en_US
dc.identifier.issue3en_US
dc.identifier.spage250en_US
dc.identifier.epage255en_US
dc.identifier.isiWOS:000280638800012-
dc.publisher.placeUnited Kingdomen_US
dc.identifier.citeulike7616476-

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