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Article: Maintenance of surrogate light chain expression induces developmental delay in early B cell compartment

TitleMaintenance of surrogate light chain expression induces developmental delay in early B cell compartment
Authors
Issue Date2007
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
Journal Of Immunology, 2007, v. 179 n. 8, p. 4996-5005 How to Cite?
AbstractThe production of a mature B cell requires passage through a number of developmental checkpoints. The pre-BCR plays a critical role in passage through the pro-B cell/pre-B cell checkpoint, and thus plays a central role in regulating the differentiation of a B cell. Due to the significance of this receptor, it is imperative that pre-BCR expression and function are precisely regulated. In this study, we have investigated a system in which the regulation of the pre-BCR is altered. We have found that continued expression of components of the pre-BCR (λ5) resulted in a delay in the kinetics of B cell maturation. Pro-B cells from normal mouse bone marrow retrovirally infected with λ5 exhibited a delay in differentiation. As compared with wild-type cells at the same time point, there is a reduction in the presence of cell surface markers that indicate developmental progression, and there is a 6- to 16-fold decrease in the production of Ig-positive cells in B cell maturation assays. The capacity to alter B cell progression by modifying and extending pre-BCR expression argues that the receptor and its associated signals play a unique role in directing developmental outcomes. Copyright © 2007 by The American Association of Immunologists, Inc.
Persistent Identifierhttp://hdl.handle.net/10722/148544
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMartin, DAen_US
dc.contributor.authorLu, Len_US
dc.contributor.authorCascalho, Men_US
dc.contributor.authorWu, GEen_US
dc.date.accessioned2012-05-29T06:13:38Z-
dc.date.available2012-05-29T06:13:38Z-
dc.date.issued2007en_US
dc.identifier.citationJournal Of Immunology, 2007, v. 179 n. 8, p. 4996-5005en_US
dc.identifier.issn0022-1767en_US
dc.identifier.urihttp://hdl.handle.net/10722/148544-
dc.description.abstractThe production of a mature B cell requires passage through a number of developmental checkpoints. The pre-BCR plays a critical role in passage through the pro-B cell/pre-B cell checkpoint, and thus plays a central role in regulating the differentiation of a B cell. Due to the significance of this receptor, it is imperative that pre-BCR expression and function are precisely regulated. In this study, we have investigated a system in which the regulation of the pre-BCR is altered. We have found that continued expression of components of the pre-BCR (λ5) resulted in a delay in the kinetics of B cell maturation. Pro-B cells from normal mouse bone marrow retrovirally infected with λ5 exhibited a delay in differentiation. As compared with wild-type cells at the same time point, there is a reduction in the presence of cell surface markers that indicate developmental progression, and there is a 6- to 16-fold decrease in the production of Ig-positive cells in B cell maturation assays. The capacity to alter B cell progression by modifying and extending pre-BCR expression argues that the receptor and its associated signals play a unique role in directing developmental outcomes. Copyright © 2007 by The American Association of Immunologists, Inc.en_US
dc.languageengen_US
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.orgen_US
dc.relation.ispartofJournal of Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshB-Lymphocyte Subsets - Immunology - Metabolism - Pathologyen_US
dc.subject.meshCell Differentiation - Genetics - Immunologyen_US
dc.subject.meshCell Line, Transformeden_US
dc.subject.meshCell Proliferationen_US
dc.subject.meshGene Expression Profilingen_US
dc.subject.meshGrowth Inhibitors - Biosynthesis - Genetics - Physiologyen_US
dc.subject.meshImmunoglobulin Light Chains, Surrogate - Biosynthesis - Genetics - Physiologyen_US
dc.subject.meshImmunophenotypingen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C57blen_US
dc.subject.meshMice, Knockouten_US
dc.subject.meshMice, Transgenicen_US
dc.subject.meshPre-B Cell Receptors - Biosynthesis - Genetics - Physiologyen_US
dc.titleMaintenance of surrogate light chain expression induces developmental delay in early B cell compartmenten_US
dc.typeArticleen_US
dc.identifier.emailLu, L:liweilu@hkucc.hku.hken_US
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid17911584en_US
dc.identifier.scopuseid_2-s2.0-38449121738en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-38449121738&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume179en_US
dc.identifier.issue8en_US
dc.identifier.spage4996en_US
dc.identifier.epage5005en_US
dc.identifier.isiWOS:000250099400006-
dc.publisher.placeUnited Statesen_US

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