File Download
  Links for fulltext
     (May Require Subscription)
Supplementary

Article: Identification of a novel tumor transforming gene GAEC1 at 7q22 which encodes a nuclear protein and is frequently amplified and overexpressed in esophageal squamous cell carcinoma.

TitleIdentification of a novel tumor transforming gene GAEC1 at 7q22 which encodes a nuclear protein and is frequently amplified and overexpressed in esophageal squamous cell carcinoma.
Authors
Issue Date2007
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2007, v. 26 n. 40, p. 5877-5888 How to Cite?
AbstractBy comparative DNA fingerprinting, we identified a 357-bp DNA fragment frequently amplified in esophageal squamous cell carcinomas (ESCC). This fragment overlaps with an expressed sequence tag mapped to 7q22. Further 5' and 3'-rapid amplification of cDNA ends revealed that it is part of a novel, single-exon gene with full-length mRNA of 2052 bp and encodes a nuclear protein of 109 amino acids ( approximately 15 kDa). This gene, designated as gene amplified in esophageal cancer 1 (GAEC1), was located within a 1-2 Mb amplicon at 7q22.1 identified by high-resolution 1 Mb array-comparative genomic hybridization in 6/10 ESCC cell lines. GAEC1 was ubiquitously expressed in normal tissues including esophageal and gastrointestinal organs; with amplification and overexpression in 6/10 (60%) ESCC cell lines and 34/99 (34%) primary tumors. Overexpression of GAEC1 in 3T3 mouse fibroblasts caused foci formation and colony formation in soft agar, comparable to H-ras and injection of GAEC1-transfected 3T3 cells into athymic nude mice formed undifferentiated sarcoma in vivo, indicating that GAEC1 is a transforming oncogene. Although no significant correlation was observed between GAEC1 amplification and clinicopathological parameters and prognosis, our study demonstrated that overexpressed GAEC1 has tumorigenic potential and suggest that overexpressed GAEC1 may play an important role in ESCC pathogenesis.
Persistent Identifierhttp://hdl.handle.net/10722/148529
ISSN
2015 Impact Factor: 7.932
2015 SCImago Journal Rankings: 4.047
ISI Accession Number ID

 

DC FieldValueLanguage
dc.contributor.authorLaw, FBen_HK
dc.contributor.authorChen, YWen_HK
dc.contributor.authorWong, KYen_HK
dc.contributor.authorYing, Jen_HK
dc.contributor.authorTao, Qen_HK
dc.contributor.authorLangford, Cen_HK
dc.contributor.authorLee, PYen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorCheung, RWen_HK
dc.contributor.authorChui, CHen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLam, KYen_HK
dc.contributor.authorWong, Jen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorTang, JCen_HK
dc.date.accessioned2012-05-29T06:13:32Z-
dc.date.available2012-05-29T06:13:32Z-
dc.date.issued2007en_HK
dc.identifier.citationOncogene, 2007, v. 26 n. 40, p. 5877-5888en_HK
dc.identifier.issn0950-9232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148529-
dc.description.abstractBy comparative DNA fingerprinting, we identified a 357-bp DNA fragment frequently amplified in esophageal squamous cell carcinomas (ESCC). This fragment overlaps with an expressed sequence tag mapped to 7q22. Further 5' and 3'-rapid amplification of cDNA ends revealed that it is part of a novel, single-exon gene with full-length mRNA of 2052 bp and encodes a nuclear protein of 109 amino acids ( approximately 15 kDa). This gene, designated as gene amplified in esophageal cancer 1 (GAEC1), was located within a 1-2 Mb amplicon at 7q22.1 identified by high-resolution 1 Mb array-comparative genomic hybridization in 6/10 ESCC cell lines. GAEC1 was ubiquitously expressed in normal tissues including esophageal and gastrointestinal organs; with amplification and overexpression in 6/10 (60%) ESCC cell lines and 34/99 (34%) primary tumors. Overexpression of GAEC1 in 3T3 mouse fibroblasts caused foci formation and colony formation in soft agar, comparable to H-ras and injection of GAEC1-transfected 3T3 cells into athymic nude mice formed undifferentiated sarcoma in vivo, indicating that GAEC1 is a transforming oncogene. Although no significant correlation was observed between GAEC1 amplification and clinicopathological parameters and prognosis, our study demonstrated that overexpressed GAEC1 has tumorigenic potential and suggest that overexpressed GAEC1 may play an important role in ESCC pathogenesis.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_HK
dc.relation.ispartofOncogeneen_HK
dc.subject.meshAmino Acid Sequenceen_US
dc.subject.meshAnimalsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCarcinoma, Squamous Cell - Genetics - Metabolismen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCell Nucleus - Metabolismen_US
dc.subject.meshChromosomes, Human, Pair 7en_US
dc.subject.meshEsophageal Neoplasms - Genetics - Metabolismen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshModels, Geneticen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.subject.meshNeoplasm Transplantationen_US
dc.subject.meshNuclear Proteins - Biosynthesis - Geneticsen_US
dc.titleIdentification of a novel tumor transforming gene GAEC1 at 7q22 which encodes a nuclear protein and is frequently amplified and overexpressed in esophageal squamous cell carcinoma.en_HK
dc.typeArticleen_HK
dc.identifier.emailLaw, S: slaw@hku.hken_HK
dc.identifier.emailTsao, SW: gswtsao@hku.hken_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.emailSrivastava, G: sgopesh@hkucc.hku.hken_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.description.naturelink_to_OA_fulltexten_US
dc.identifier.doi10.1038/sj.onc.1210390en_HK
dc.identifier.pmid17384685-
dc.identifier.scopuseid_2-s2.0-35148824989en_HK
dc.identifier.hkuros135307-
dc.identifier.volume26en_HK
dc.identifier.issue40en_HK
dc.identifier.spage5877en_HK
dc.identifier.epage5888en_HK
dc.identifier.isiWOS:000249123100005-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLaw, FB=12792449100en_HK
dc.identifier.scopusauthoridChen, YW=7601441119en_HK
dc.identifier.scopusauthoridWong, KY=7404758500en_HK
dc.identifier.scopusauthoridYing, J=12645439800en_HK
dc.identifier.scopusauthoridTao, Q=7102578359en_HK
dc.identifier.scopusauthoridLangford, C=7102621963en_HK
dc.identifier.scopusauthoridLee, PY=8731985700en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridCheung, RW=35081731300en_HK
dc.identifier.scopusauthoridChui, CH=7102093724en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLam, KY=7403657165en_HK
dc.identifier.scopusauthoridWong, J=8049324500en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridTang, JC=14056850300en_HK
dc.identifier.citeulike1190203-

Export via OAI-PMH Interface in XML Formats


OR


Export to Other Non-XML Formats