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Article: Identification and Characterization of Tumorigenic Liver Cancer Stem/Progenitor Cells

TitleIdentification and Characterization of Tumorigenic Liver Cancer Stem/Progenitor Cells
Authors
Issue Date2007
PublisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastro
Citation
Gastroenterology, 2007, v. 132 n. 7, p. 2542-2556 How to Cite?
AbstractBackground & Aims: Recent efforts in stem cell biology suggest that tumors are organized in a hierarchy of heterogeneous cell populations and that the capability to maintain tumor formation/growth specifically resides in a small population of cells called cancer stem cells (CSCs). The aim of this study is to identify, isolate, and characterize the CSC population that drives and maintains hepatocellular carcinoma (HCC) growth and metastasis. Methods: Normal stem cells involved in liver regeneration were identified using a severe partial hepatectomy model. Purified HCC cells, with or without expression of the identified normal stem cell phenotype, were evaluated, based on their tumorigenic potential and exhibition of defined stem/progenitor cell-like properties, to determine whether liver CSCs can be or partly be identified by this surface marker. Results: We report the identification and isolation of a population of CSCs expressing a CD133 surface phenotype from human liver cell lines. CD133+ cells possess a greater colony-forming efficiency, higher proliferative output, and greater ability to form tumor in vivo. These cells are endowed with characteristics similar to those of progenitor cells including the expression of "stemness" genes, the ability to self-renew, and the ability to differentiate into nonhepatocyte-like lineages. Furthermore, CD133 is found to represent only a minority of the tumor cell population in human HCC specimens. Conclusions: We report the identification of a CSC population in HCC characterized by their CD133 phenotype. The identification of tumorigenic liver CSCs could provide new insight into the HCC tumorigenic process and possibly bear great therapeutic implications. © 2007 AGA Institute.
Persistent Identifierhttp://hdl.handle.net/10722/148513
ISSN
2015 Impact Factor: 18.187
2015 SCImago Journal Rankings: 7.170
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorMa, Sen_HK
dc.contributor.authorChan, Ken_HK
dc.contributor.authorHu, Len_HK
dc.contributor.authorLee, TKen_HK
dc.contributor.authorWo, JYen_HK
dc.contributor.authorNg, IOen_HK
dc.contributor.authorZheng, Ben_HK
dc.contributor.authorGuan, Xen_HK
dc.date.accessioned2012-05-29T06:13:25Z-
dc.date.available2012-05-29T06:13:25Z-
dc.date.issued2007en_HK
dc.identifier.citationGastroenterology, 2007, v. 132 n. 7, p. 2542-2556en_HK
dc.identifier.issn0016-5085en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148513-
dc.description.abstractBackground & Aims: Recent efforts in stem cell biology suggest that tumors are organized in a hierarchy of heterogeneous cell populations and that the capability to maintain tumor formation/growth specifically resides in a small population of cells called cancer stem cells (CSCs). The aim of this study is to identify, isolate, and characterize the CSC population that drives and maintains hepatocellular carcinoma (HCC) growth and metastasis. Methods: Normal stem cells involved in liver regeneration were identified using a severe partial hepatectomy model. Purified HCC cells, with or without expression of the identified normal stem cell phenotype, were evaluated, based on their tumorigenic potential and exhibition of defined stem/progenitor cell-like properties, to determine whether liver CSCs can be or partly be identified by this surface marker. Results: We report the identification and isolation of a population of CSCs expressing a CD133 surface phenotype from human liver cell lines. CD133+ cells possess a greater colony-forming efficiency, higher proliferative output, and greater ability to form tumor in vivo. These cells are endowed with characteristics similar to those of progenitor cells including the expression of "stemness" genes, the ability to self-renew, and the ability to differentiate into nonhepatocyte-like lineages. Furthermore, CD133 is found to represent only a minority of the tumor cell population in human HCC specimens. Conclusions: We report the identification of a CSC population in HCC characterized by their CD133 phenotype. The identification of tumorigenic liver CSCs could provide new insight into the HCC tumorigenic process and possibly bear great therapeutic implications. © 2007 AGA Institute.en_HK
dc.languageengen_US
dc.publisherWB Saunders Co. The Journal's web site is located at http://www.elsevier.com/locate/gastroen_HK
dc.relation.ispartofGastroenterologyen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshAntigens, Cd - Metabolismen_US
dc.subject.meshAntigens, Differentiation - Metabolismen_US
dc.subject.meshCarcinogenicity Testsen_US
dc.subject.meshCarcinoma, Hepatocellular - Metabolism - Pathologyen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Divisionen_US
dc.subject.meshCell Line, Tumoren_US
dc.subject.meshCell Separationen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshGlycoproteins - Metabolismen_US
dc.subject.meshHumansen_US
dc.subject.meshLiver - Metabolism - Pathology - Physiopathologyen_US
dc.subject.meshLiver Neoplasms - Metabolism - Pathologyen_US
dc.subject.meshLiver Regenerationen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshMice, Sciden_US
dc.subject.meshMuscle Developmenten_US
dc.subject.meshNeoplasm Transplantationen_US
dc.subject.meshNeovascularization, Pathologicen_US
dc.subject.meshPeptides - Metabolismen_US
dc.subject.meshPhenotypeen_US
dc.subject.meshStem Cells - Metabolism - Pathologyen_US
dc.subject.meshTransplantation, Heterologousen_US
dc.titleIdentification and Characterization of Tumorigenic Liver Cancer Stem/Progenitor Cellsen_HK
dc.typeArticleen_HK
dc.identifier.emailMa, S:sma@pathology.hku.hken_HK
dc.identifier.emailChan, K:hrmtckw@hku.hken_HK
dc.identifier.emailLee, TK:tkwlee@hkucc.hku.hken_HK
dc.identifier.emailNg, IO:iolng@hkucc.hku.hken_HK
dc.identifier.emailZheng, B:bzheng@hkucc.hku.hken_HK
dc.identifier.emailGuan, X:xyguan@hkucc.hku.hken_HK
dc.identifier.authorityMa, S=rp00506en_HK
dc.identifier.authorityChan, K=rp00330en_HK
dc.identifier.authorityLee, TK=rp00447en_HK
dc.identifier.authorityNg, IO=rp00335en_HK
dc.identifier.authorityZheng, B=rp00353en_HK
dc.identifier.authorityGuan, X=rp00454en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1053/j.gastro.2007.04.025en_HK
dc.identifier.pmid17570225-
dc.identifier.scopuseid_2-s2.0-34249980343en_HK
dc.identifier.hkuros129934-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-34249980343&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume132en_HK
dc.identifier.issue7en_HK
dc.identifier.spage2542en_HK
dc.identifier.epage2556en_HK
dc.identifier.eissn1528-0012-
dc.identifier.isiWOS:000247146900031-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridMa, S=16444895800en_HK
dc.identifier.scopusauthoridChan, K=16444133100en_HK
dc.identifier.scopusauthoridHu, L=34770075600en_HK
dc.identifier.scopusauthoridLee, TK=7501439435en_HK
dc.identifier.scopusauthoridWo, JY=7003466728en_HK
dc.identifier.scopusauthoridNg, IO=7102753722en_HK
dc.identifier.scopusauthoridZheng, B=7201780588en_HK
dc.identifier.scopusauthoridGuan, X=7201463221en_HK

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