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Article: Identification of a tumor suppressive critical region mapping to 3p14.2 in esophageal squamous cell carcinoma and studies of a candidate tumor suppressor gene, ADAMTS9

TitleIdentification of a tumor suppressive critical region mapping to 3p14.2 in esophageal squamous cell carcinoma and studies of a candidate tumor suppressor gene, ADAMTS9
Authors
KeywordsADAMTS9
Chromosome 3
Esophageal carcinoma
Microcell-mediated chromosome transfer
Tumor suppressor gene
Issue Date2007
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2007, v. 26 n. 1, p. 148-157 How to Cite?
AbstractA gene critical to esophageal cancer has been identified. Functional studies using microcell-mediated chromosome transfer of intact and truncated donor chromosomes 3 into an esophageal cancer cell line and nude mouse tumorigenicity assays were used to identify a 1.61 Mb tumor suppressive critical region (CR) mapping to chromosome 3p14.2. This CR is bounded by D3S1600 and D3S1285 microsatellite markers. One candidate tumor suppressor gene, ADAMTS9, maps to this CR. Further studies showed normal expression levels of this gene in tumor-suppressed microcell hybrids, levels that were much higher than observed in the recipient cells. Complete loss or downregulation of ADAMTS9 gene expression was found in 15 out of 16 esophageal carcinoma cell lines. Promoter hypermethylation was detected in the cell lines that do not express this gene. Re-expression of ADAMTS9 was observed after demethylation drug treatment, confirming that hypermethylation is involved in gene downregulation. Downregulation of ADAMTS9 was also found in 43.5 and 47.6% of primary esophageal tumor tissues from Hong Kong and from the high-risk region of Henan, respectively. Thus, this study identifies and provides functional evidence for a CR associated with tumor suppression on 3p14.2 and provides the first evidence that ADAMTS9, mapping to this region, may contribute to esophageal cancer development. © 2007 Nature Publishing Group All rights reserved.
Persistent Identifierhttp://hdl.handle.net/10722/148498
ISSN
2015 Impact Factor: 7.932
2015 SCImago Journal Rankings: 4.047
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLo, PHYen_HK
dc.contributor.authorLeung, ACCen_HK
dc.contributor.authorKwok, CYCen_HK
dc.contributor.authorCheung, WSYen_HK
dc.contributor.authorKo, JMYen_HK
dc.contributor.authorYang, LCen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorWang, LDen_HK
dc.contributor.authorLi, Jen_HK
dc.contributor.authorStanbridge, EJen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorTang, JCOen_HK
dc.contributor.authorTsao, SWen_HK
dc.contributor.authorLung, MLen_HK
dc.date.accessioned2012-05-29T06:13:19Z-
dc.date.available2012-05-29T06:13:19Z-
dc.date.issued2007en_HK
dc.identifier.citationOncogene, 2007, v. 26 n. 1, p. 148-157en_HK
dc.identifier.issn0950-9232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148498-
dc.description.abstractA gene critical to esophageal cancer has been identified. Functional studies using microcell-mediated chromosome transfer of intact and truncated donor chromosomes 3 into an esophageal cancer cell line and nude mouse tumorigenicity assays were used to identify a 1.61 Mb tumor suppressive critical region (CR) mapping to chromosome 3p14.2. This CR is bounded by D3S1600 and D3S1285 microsatellite markers. One candidate tumor suppressor gene, ADAMTS9, maps to this CR. Further studies showed normal expression levels of this gene in tumor-suppressed microcell hybrids, levels that were much higher than observed in the recipient cells. Complete loss or downregulation of ADAMTS9 gene expression was found in 15 out of 16 esophageal carcinoma cell lines. Promoter hypermethylation was detected in the cell lines that do not express this gene. Re-expression of ADAMTS9 was observed after demethylation drug treatment, confirming that hypermethylation is involved in gene downregulation. Downregulation of ADAMTS9 was also found in 43.5 and 47.6% of primary esophageal tumor tissues from Hong Kong and from the high-risk region of Henan, respectively. Thus, this study identifies and provides functional evidence for a CR associated with tumor suppression on 3p14.2 and provides the first evidence that ADAMTS9, mapping to this region, may contribute to esophageal cancer development. © 2007 Nature Publishing Group All rights reserved.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_HK
dc.relation.ispartofOncogeneen_HK
dc.subjectADAMTS9en_HK
dc.subjectChromosome 3en_HK
dc.subjectEsophageal carcinomaen_HK
dc.subjectMicrocell-mediated chromosome transferen_HK
dc.subjectTumor suppressor geneen_HK
dc.subject.meshAdam Proteins - Geneticsen_US
dc.subject.meshBase Sequenceen_US
dc.subject.meshCarcinoma, Squamous Cell - Geneticsen_US
dc.subject.meshChromosome Mappingen_US
dc.subject.meshChromosomes, Human, Pair 3en_US
dc.subject.meshDnaen_US
dc.subject.meshDna Methylationen_US
dc.subject.meshEsophageal Neoplasms - Geneticsen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshGenes, Tumor Suppressoren_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshMolecular Sequence Dataen_US
dc.titleIdentification of a tumor suppressive critical region mapping to 3p14.2 in esophageal squamous cell carcinoma and studies of a candidate tumor suppressor gene, ADAMTS9en_HK
dc.typeArticleen_HK
dc.identifier.emailLaw, S:slaw@hku.hken_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.emailTsao, SW:gswtsao@hkucc.hku.hken_HK
dc.identifier.emailLung, ML:mlilung@hku.hken_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authorityTsao, SW=rp00399en_HK
dc.identifier.authorityLung, ML=rp00300en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.onc.1209767en_HK
dc.identifier.pmid16799631-
dc.identifier.scopuseid_2-s2.0-33846046177en_HK
dc.identifier.hkuros140863-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-33846046177&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume26en_HK
dc.identifier.issue1en_HK
dc.identifier.spage148en_HK
dc.identifier.epage157en_HK
dc.identifier.isiWOS:000243236500015-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridLo, PHY=36762664000en_HK
dc.identifier.scopusauthoridLeung, ACC=15760220500en_HK
dc.identifier.scopusauthoridKwok, CYC=15760014400en_HK
dc.identifier.scopusauthoridCheung, WSY=15759017200en_HK
dc.identifier.scopusauthoridKo, JMY=35725559400en_HK
dc.identifier.scopusauthoridYang, LC=7406280398en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridWang, LD=12242861000en_HK
dc.identifier.scopusauthoridLi, J=27168754300en_HK
dc.identifier.scopusauthoridStanbridge, EJ=7103249410en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridTang, JCO=14056850300en_HK
dc.identifier.scopusauthoridTsao, SW=7102813116en_HK
dc.identifier.scopusauthoridLung, ML=7006411788en_HK
dc.identifier.citeulike714396-

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