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Article: Tumor suppressive role of a 2.4 Mb 9q33-q34 critical region and DEC1 in esophageal squamous cell carcinoma

TitleTumor suppressive role of a 2.4 Mb 9q33-q34 critical region and DEC1 in esophageal squamous cell carcinoma
Authors
KeywordsChromosome 9
Deleted in esophageal cancer 1
Esophageal carcinoma
Microcell-mediated chromosome transfer
Tumor suppressor gene
Issue Date2005
PublisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/onc
Citation
Oncogene, 2005, v. 24 n. 4, p. 697-705 How to Cite?
AbstractThe key genes involved in the development of esophageal squamous cell carcinoma (ESCC) remain to be elucidated. Previous studies indicate extensive genomic alterations occur on chromosome 9 in ESCC. Using a monochromosome transfer approach, this study provides functional evidence and narrows down the critical region (CR) responsible for chromosome 9 tumor suppressing activity to a 2.4 Mb region mapping to 9q33-q34 between markers D9S1798 and D9S61. Interestingly, a high prevalence of allelic loss in this CR is also observed in primary ESCC tumors by microsatellite typing. Allelic loss is found in 30/34 (88%) tumors and the loss of heterozygosity (LOH) frequency ranges from 67 to 86%. Absent to low expression of a 9q32 candidate tumor suppressor gene (TSG), DEC1 (deleted in esophageal cancer 1), is detected in four Asian ESCC cell lines. Stably expressing DEC1 transfectants provide functional evidence for inhibition of tumor growth in nude mice and DEC1 expression is decreased in tumor segregants arising after long-term selection in vivo. There is 74% LOH in the DEC1 region of ESCC primary tumors. This study provides the first functional evidence for the presence of critical tumor suppressive regions on 9q33-q34. DEC1 is a candidate TSG that may be involved in ESCC development.
Persistent Identifierhttp://hdl.handle.net/10722/148413
ISSN
2015 Impact Factor: 7.932
2015 SCImago Journal Rankings: 4.047
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorYang, Len_HK
dc.contributor.authorLeung, ACCen_HK
dc.contributor.authorKo, JMYen_HK
dc.contributor.authorLo, PHYen_HK
dc.contributor.authorTang, JCOen_HK
dc.contributor.authorSrivastava, Gen_HK
dc.contributor.authorOshimura, Men_HK
dc.contributor.authorStanbridge, EJen_HK
dc.contributor.authorDaigo, Yen_HK
dc.contributor.authorNakamura, Yen_HK
dc.contributor.authorTang, CMCen_HK
dc.contributor.authorLau, KWen_HK
dc.contributor.authorLaw, Sen_HK
dc.contributor.authorLung, MLen_HK
dc.date.accessioned2012-05-29T06:12:49Z-
dc.date.available2012-05-29T06:12:49Z-
dc.date.issued2005en_HK
dc.identifier.citationOncogene, 2005, v. 24 n. 4, p. 697-705en_HK
dc.identifier.issn0950-9232en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148413-
dc.description.abstractThe key genes involved in the development of esophageal squamous cell carcinoma (ESCC) remain to be elucidated. Previous studies indicate extensive genomic alterations occur on chromosome 9 in ESCC. Using a monochromosome transfer approach, this study provides functional evidence and narrows down the critical region (CR) responsible for chromosome 9 tumor suppressing activity to a 2.4 Mb region mapping to 9q33-q34 between markers D9S1798 and D9S61. Interestingly, a high prevalence of allelic loss in this CR is also observed in primary ESCC tumors by microsatellite typing. Allelic loss is found in 30/34 (88%) tumors and the loss of heterozygosity (LOH) frequency ranges from 67 to 86%. Absent to low expression of a 9q32 candidate tumor suppressor gene (TSG), DEC1 (deleted in esophageal cancer 1), is detected in four Asian ESCC cell lines. Stably expressing DEC1 transfectants provide functional evidence for inhibition of tumor growth in nude mice and DEC1 expression is decreased in tumor segregants arising after long-term selection in vivo. There is 74% LOH in the DEC1 region of ESCC primary tumors. This study provides the first functional evidence for the presence of critical tumor suppressive regions on 9q33-q34. DEC1 is a candidate TSG that may be involved in ESCC development.en_HK
dc.languageengen_US
dc.publisherNature Publishing Group. The Journal's web site is located at http://www.nature.com/oncen_HK
dc.relation.ispartofOncogeneen_HK
dc.subjectChromosome 9en_HK
dc.subjectDeleted in esophageal cancer 1en_HK
dc.subjectEsophageal carcinomaen_HK
dc.subjectMicrocell-mediated chromosome transferen_HK
dc.subjectTumor suppressor geneen_HK
dc.subject.meshAnimalsen_US
dc.subject.meshCarcinogenicity Testsen_US
dc.subject.meshCarcinoma, Squamous Cell - Genetics - Metabolism - Pathologyen_US
dc.subject.meshChromosome Deletionen_US
dc.subject.meshChromosomes, Human, Pair 18 - Geneticsen_US
dc.subject.meshChromosomes, Human, Pair 9 - Geneticsen_US
dc.subject.meshDna, Complementary - Geneticsen_US
dc.subject.meshEsophageal Neoplasms - Genetics - Metabolism - Pathologyen_US
dc.subject.meshFemaleen_US
dc.subject.meshGene Expression Regulation, Neoplasticen_US
dc.subject.meshHumansen_US
dc.subject.meshIn Situ Hybridization, Fluorescenceen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Nudeen_US
dc.subject.meshNeoplasm Transplantationen_US
dc.subject.meshTumor Cells, Cultureden_US
dc.subject.meshTumor Suppressor Proteins - Genetics - Metabolismen_US
dc.titleTumor suppressive role of a 2.4 Mb 9q33-q34 critical region and DEC1 in esophageal squamous cell carcinomaen_HK
dc.typeArticleen_HK
dc.identifier.emailSrivastava, G:gopesh@pathology.hku.hken_HK
dc.identifier.emailLaw, S:slaw@hku.hken_HK
dc.identifier.emailLung, ML:mlilung@hku.hken_HK
dc.identifier.authoritySrivastava, G=rp00365en_HK
dc.identifier.authorityLaw, S=rp00437en_HK
dc.identifier.authorityLung, ML=rp00300en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1038/sj.onc.1208179en_HK
dc.identifier.pmid15580306-
dc.identifier.scopuseid_2-s2.0-19944430844en_HK
dc.identifier.hkuros99974-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-19944430844&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume24en_HK
dc.identifier.issue4en_HK
dc.identifier.spage697en_HK
dc.identifier.epage705en_HK
dc.identifier.isiWOS:000226420400017-
dc.publisher.placeUnited Kingdomen_HK
dc.identifier.scopusauthoridYang, L=14827615000en_HK
dc.identifier.scopusauthoridLeung, ACC=15760220500en_HK
dc.identifier.scopusauthoridKo, JMY=35725559400en_HK
dc.identifier.scopusauthoridLo, PHY=36762664000en_HK
dc.identifier.scopusauthoridTang, JCO=14056850300en_HK
dc.identifier.scopusauthoridSrivastava, G=7202242238en_HK
dc.identifier.scopusauthoridOshimura, M=7102847689en_HK
dc.identifier.scopusauthoridStanbridge, EJ=7103249410en_HK
dc.identifier.scopusauthoridDaigo, Y=7004496677en_HK
dc.identifier.scopusauthoridNakamura, Y=36013862000en_HK
dc.identifier.scopusauthoridTang, CMC=8274797200en_HK
dc.identifier.scopusauthoridLau, KW=35080643000en_HK
dc.identifier.scopusauthoridLaw, S=7202241293en_HK
dc.identifier.scopusauthoridLung, ML=7006411788en_HK
dc.identifier.citeulike2155-

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