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Article: Distinct intragraft response pattern in relation to graft size in liver transplantation

TitleDistinct intragraft response pattern in relation to graft size in liver transplantation
Authors
Issue Date2003
PublisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.transplantjournal.com
Citation
Transplantation, 2003, v. 75 n. 5, p. 673-678 How to Cite?
AbstractBackground. The molecular mechanism of small-forsize graft injury remains unclear. The aim of this study is to investigate the gene expression pattern of acute phase response in relation to graft size in a rat-liver transplantation model. Methods. A rat orthotopic liver transplantation model using 30%, 50%, and whole grafts was used. The graft survival rates and liver morphology were compared among the three groups. Two transcription factors, nuclear factor (NF)-κB (p65) and early growth response (Egr-1), and their downstream genes were compared. Results. According to the graft size, the rats were grouped as follows: group 1 (n=20), 32% (24-47%); group 2 (n=10), 56% (50-65%); and group 3 (n=10), 104% (89-120%). The 7-day survival rates were 20% (P=0.039 vs. group 2, P=0.000 vs. group 3), 60%, and 100% in groups 1, 2, and 3, respectively. Dilation of hepatic sinusoids and vacuolization of hepatocytes were observed in group 1. Up-regulation of Egr-1 and endothelin (ET)-1 and over-expression of nitric oxide synthase (iNOS) was found in group 1, but heme oxygenase (HO)-1 and A20 were down-regulated. At 24 hours after reperfusion, the intragraft protein level of heat-shock protein (Hsp)-70 was significantly lower in group 1 than that in group 3 (12.4 vs. 17.0 ng/mL, P=0.04). More apoptotic nuclei were found in group 1. Conclusions. Small-for-size graft injury was related to early over-expression of Egr-1 associated with upregulation of ET-1 and deterioration of intracellular homeostasis reflected by down-regulation of Hsps and A20.
Persistent Identifierhttp://hdl.handle.net/10722/148329
ISSN
2015 Impact Factor: 3.69
2015 SCImago Journal Rankings: 1.699
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLiang, TBen_HK
dc.contributor.authorMan, Ken_HK
dc.contributor.authorLee, TKWen_HK
dc.contributor.authorTsui, SHTen_HK
dc.contributor.authorLo, CMen_HK
dc.contributor.authorXu, Xen_HK
dc.contributor.authorZheng, SSen_HK
dc.contributor.authorFan, STen_HK
dc.contributor.authorWong, Jen_HK
dc.date.accessioned2012-05-29T06:12:15Z-
dc.date.available2012-05-29T06:12:15Z-
dc.date.issued2003en_HK
dc.identifier.citationTransplantation, 2003, v. 75 n. 5, p. 673-678en_HK
dc.identifier.issn0041-1337en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148329-
dc.description.abstractBackground. The molecular mechanism of small-forsize graft injury remains unclear. The aim of this study is to investigate the gene expression pattern of acute phase response in relation to graft size in a rat-liver transplantation model. Methods. A rat orthotopic liver transplantation model using 30%, 50%, and whole grafts was used. The graft survival rates and liver morphology were compared among the three groups. Two transcription factors, nuclear factor (NF)-κB (p65) and early growth response (Egr-1), and their downstream genes were compared. Results. According to the graft size, the rats were grouped as follows: group 1 (n=20), 32% (24-47%); group 2 (n=10), 56% (50-65%); and group 3 (n=10), 104% (89-120%). The 7-day survival rates were 20% (P=0.039 vs. group 2, P=0.000 vs. group 3), 60%, and 100% in groups 1, 2, and 3, respectively. Dilation of hepatic sinusoids and vacuolization of hepatocytes were observed in group 1. Up-regulation of Egr-1 and endothelin (ET)-1 and over-expression of nitric oxide synthase (iNOS) was found in group 1, but heme oxygenase (HO)-1 and A20 were down-regulated. At 24 hours after reperfusion, the intragraft protein level of heat-shock protein (Hsp)-70 was significantly lower in group 1 than that in group 3 (12.4 vs. 17.0 ng/mL, P=0.04). More apoptotic nuclei were found in group 1. Conclusions. Small-for-size graft injury was related to early over-expression of Egr-1 associated with upregulation of ET-1 and deterioration of intracellular homeostasis reflected by down-regulation of Hsps and A20.en_HK
dc.languageengen_US
dc.publisherLippincott Williams & Wilkins. The Journal's web site is located at http://www.transplantjournal.comen_HK
dc.relation.ispartofTransplantationen_HK
dc.rightsTransplantation. Copyright © Lippincott Williams & Wilkins.-
dc.subject.meshAcute-Phase Reactionen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshDna-Binding Proteins - Metabolismen_US
dc.subject.meshEarly Growth Response Protein 1en_US
dc.subject.meshEndothelin-1 - Geneticsen_US
dc.subject.meshGene Expressionen_US
dc.subject.meshHsp70 Heat-Shock Proteins - Geneticsen_US
dc.subject.meshHeme Oxygenase (Decyclizing) - Geneticsen_US
dc.subject.meshHeme Oxygenase-1en_US
dc.subject.meshImmediate-Early Proteinsen_US
dc.subject.meshLiver - Anatomy & Histology - Pathology - Physiopathologyen_US
dc.subject.meshLiver Transplantationen_US
dc.subject.meshMaleen_US
dc.subject.meshNf-Kappa B - Metabolismen_US
dc.subject.meshNitric Oxide Synthase - Metabolismen_US
dc.subject.meshNitric Oxide Synthase Type Iien_US
dc.subject.meshOrgan Sizeen_US
dc.subject.meshProteins - Geneticsen_US
dc.subject.meshRatsen_US
dc.subject.meshRats, Inbred Lewen_US
dc.subject.meshSurvival Analysisen_US
dc.subject.meshTranscription Factors - Metabolismen_US
dc.subject.meshVasoconstriction - Physiologyen_US
dc.titleDistinct intragraft response pattern in relation to graft size in liver transplantationen_HK
dc.typeArticleen_HK
dc.identifier.emailMan, K: kwanman@hku.hken_HK
dc.identifier.emailLee, TKW: tkwlee@hkucc.hku.hken_HK
dc.identifier.emailLo, CM: chungmlo@hkucc.hku.hken_HK
dc.identifier.emailFan, ST: stfan@hku.hken_HK
dc.identifier.emailWong, J: jwong@hkucc.hku.hken_HK
dc.identifier.authorityMan, K=rp00417en_HK
dc.identifier.authorityLee, TKW=rp00447en_HK
dc.identifier.authorityLo, CM=rp00412en_HK
dc.identifier.authorityFan, ST=rp00355en_HK
dc.identifier.authorityWong, J=rp00322en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1097/01.TP.0000048490.24429.89en_HK
dc.identifier.pmid12640308-
dc.identifier.scopuseid_2-s2.0-0037444070en_HK
dc.identifier.hkuros76917-
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0037444070&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume75en_HK
dc.identifier.issue5en_HK
dc.identifier.spage673en_HK
dc.identifier.epage678en_HK
dc.identifier.isiWOS:000181687600017-
dc.publisher.placeUnited Statesen_HK
dc.identifier.scopusauthoridLiang, TB=7202019213en_HK
dc.identifier.scopusauthoridMan, K=7101754072en_HK
dc.identifier.scopusauthoridLee, TKW=7501439435en_HK
dc.identifier.scopusauthoridTsui, SHT=36887263200en_HK
dc.identifier.scopusauthoridLo, CM=7401771672en_HK
dc.identifier.scopusauthoridXu, X=7405294857en_HK
dc.identifier.scopusauthoridZheng, SS=7403146419en_HK
dc.identifier.scopusauthoridFan, ST=7402678224en_HK
dc.identifier.scopusauthoridWong, J=8049324500en_HK

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