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Article: Regulation of cell survival during B lymphopoiesis: Suppressed apoptosis of pro-B cells in P53-deficient mouse bone marrow

TitleRegulation of cell survival during B lymphopoiesis: Suppressed apoptosis of pro-B cells in P53-deficient mouse bone marrow
Authors
Issue Date1999
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de
Citation
European Journal Of Immunology, 1999, v. 29 n. 8, p. 2484-2490 How to Cite?
AbstractB cell development in mouse bone marrow (BM) is subject to quality controls that eliminate aberrant cells by apoptosis, but the intrinsic cellular mechanisms that mediate this negative B cell selection remain unclear. The p53 tumor suppressor transduces signals resulting in apoptosis and cell cycle arrest in cells that sustain DNA damage. Faulty V(D)J recombination in scid lymphocyte precursors activates a p53-dependent DNA damage checkpoint. In the present study, we have examined whether p53 is involved in apoptotic selection of normally developing B cells in BM. Double immunofluorescence labeling and flow cytometry were used to quantitate phenotypically defined B cell populations and their apoptotic rates in BM of homozygous p53-deficient mice. B220+ μ- and terminal deoxynucleotidyl transferase (TdT)+ pro-B cells were increased in both incidence and absolute number to controls. In contrast, pre-B cells were only slightly increased and the sIgM+ B lymphocyte compartment remained essentially normal. The incidence of apoptosis among p53(-/-) pro-B cells was greatly reduced, both ex vivo and in short-term culture, whereas, apoptosis of pre-B cells and B lymphocytes was not significantly different from normal. The results indicate that p53 is actively involved as an apoptosis inducer at an early quality control checkpoint in B lymphopoiesis.
Persistent Identifierhttp://hdl.handle.net/10722/148138
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Len_US
dc.contributor.authorLejtenyi, Den_US
dc.contributor.authorOsmond, DGen_US
dc.date.accessioned2012-05-29T06:11:04Z-
dc.date.available2012-05-29T06:11:04Z-
dc.date.issued1999en_US
dc.identifier.citationEuropean Journal Of Immunology, 1999, v. 29 n. 8, p. 2484-2490en_US
dc.identifier.issn0014-2980en_US
dc.identifier.urihttp://hdl.handle.net/10722/148138-
dc.description.abstractB cell development in mouse bone marrow (BM) is subject to quality controls that eliminate aberrant cells by apoptosis, but the intrinsic cellular mechanisms that mediate this negative B cell selection remain unclear. The p53 tumor suppressor transduces signals resulting in apoptosis and cell cycle arrest in cells that sustain DNA damage. Faulty V(D)J recombination in scid lymphocyte precursors activates a p53-dependent DNA damage checkpoint. In the present study, we have examined whether p53 is involved in apoptotic selection of normally developing B cells in BM. Double immunofluorescence labeling and flow cytometry were used to quantitate phenotypically defined B cell populations and their apoptotic rates in BM of homozygous p53-deficient mice. B220+ μ- and terminal deoxynucleotidyl transferase (TdT)+ pro-B cells were increased in both incidence and absolute number to controls. In contrast, pre-B cells were only slightly increased and the sIgM+ B lymphocyte compartment remained essentially normal. The incidence of apoptosis among p53(-/-) pro-B cells was greatly reduced, both ex vivo and in short-term culture, whereas, apoptosis of pre-B cells and B lymphocytes was not significantly different from normal. The results indicate that p53 is actively involved as an apoptosis inducer at an early quality control checkpoint in B lymphopoiesis.en_US
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.deen_US
dc.relation.ispartofEuropean Journal of Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshB-Lymphocytes - Cytology - Immunology - Metabolismen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Survivalen_US
dc.subject.meshGenes, P53en_US
dc.subject.meshHematopoietic Stem Cells - Cytology - Immunology - Metabolismen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Knockouten_US
dc.subject.meshTumor Suppressor Protein P53 - Deficiency - Geneticsen_US
dc.titleRegulation of cell survival during B lymphopoiesis: Suppressed apoptosis of pro-B cells in P53-deficient mouse bone marrowen_US
dc.typeArticleen_US
dc.identifier.emailLu, L:liweilu@hkucc.hku.hken_US
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1521-4141(199908)29:08<2484::AID-IMMU2484>3.0.CO;2-Ben_US
dc.identifier.pmid10458762en_US
dc.identifier.scopuseid_2-s2.0-0032773042en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0032773042&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume29en_US
dc.identifier.issue8en_US
dc.identifier.spage2484en_US
dc.identifier.epage2490en_US
dc.identifier.isiWOS:000082042500015-
dc.publisher.placeGermanyen_US

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