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Article: Two models of murine B lymphopoiesis: A correlation

TitleTwo models of murine B lymphopoiesis: A correlation
Authors
Issue Date1998
PublisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.de
Citation
European Journal Of Immunology, 1998, v. 28 n. 6, p. 1755-1761 How to Cite?
AbstractDuring B cell genesis in mouse bone marrow (BM), precursor B cells pass through a series of developmental stages that have been defined by changes in expression of various marker molecules. The use of dissimilar phenotypic criteria in different laboratories, however, has led to the formulation of disparate models of B lymphopoiesis not fully reconciled with one another. We have directly compared two such models, one based on expression of intracellular μ heavy chain of IgM (cμ) and terminal deoxynucleotidyl transferase (TdT), the other monitoring cell surface leukosialin (CD43), heat-stable antigen (HSA; CD24) and the ectopeptidase BP-1. Each model uses cell surface B220 glycoprotein (CD45RA) to denote the B cell lineage. We have examined the cellular composition of four sorted BM fractions by immunofluorescent labeling of CD43, HSA and BP-1, using immunofluorescence microscopy of cytocentrifuged fractions to quantitate precursor B cell populations expressing either cμ or TdT. The results reveal a range of B cell differentiation stages within individual sorted BM fractions, providing a cross-reference between these two analytical methods and contributing to a unified model of B cell development in mouse BM.
Persistent Identifierhttp://hdl.handle.net/10722/148112
ISSN
2015 Impact Factor: 4.179
2015 SCImago Journal Rankings: 2.568
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Len_US
dc.contributor.authorSmithson, Gen_US
dc.contributor.authorKincade, PWen_US
dc.contributor.authorOsmond, DGen_US
dc.date.accessioned2012-05-29T06:10:54Z-
dc.date.available2012-05-29T06:10:54Z-
dc.date.issued1998en_US
dc.identifier.citationEuropean Journal Of Immunology, 1998, v. 28 n. 6, p. 1755-1761en_US
dc.identifier.issn0014-2980en_US
dc.identifier.urihttp://hdl.handle.net/10722/148112-
dc.description.abstractDuring B cell genesis in mouse bone marrow (BM), precursor B cells pass through a series of developmental stages that have been defined by changes in expression of various marker molecules. The use of dissimilar phenotypic criteria in different laboratories, however, has led to the formulation of disparate models of B lymphopoiesis not fully reconciled with one another. We have directly compared two such models, one based on expression of intracellular μ heavy chain of IgM (cμ) and terminal deoxynucleotidyl transferase (TdT), the other monitoring cell surface leukosialin (CD43), heat-stable antigen (HSA; CD24) and the ectopeptidase BP-1. Each model uses cell surface B220 glycoprotein (CD45RA) to denote the B cell lineage. We have examined the cellular composition of four sorted BM fractions by immunofluorescent labeling of CD43, HSA and BP-1, using immunofluorescence microscopy of cytocentrifuged fractions to quantitate precursor B cell populations expressing either cμ or TdT. The results reveal a range of B cell differentiation stages within individual sorted BM fractions, providing a cross-reference between these two analytical methods and contributing to a unified model of B cell development in mouse BM.en_US
dc.languageengen_US
dc.publisherWiley - V C H Verlag GmbH & Co KGaA. The Journal's web site is located at http://www.eji.deen_US
dc.relation.ispartofEuropean Journal of Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshB-Lymphocytes - Cytology - Metabolismen_US
dc.subject.meshBone Marrow Cellsen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCell Separationen_US
dc.subject.meshDna Nucleotidylexotransferase - Biosynthesisen_US
dc.subject.meshFlow Cytometryen_US
dc.subject.meshImmunoglobulin Mu-Chains - Biosynthesisen_US
dc.subject.meshImmunophenotypingen_US
dc.subject.meshLeukopoiesisen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred Balb Cen_US
dc.subject.meshMicroscopy, Fluorescenceen_US
dc.subject.meshModels, Immunologicalen_US
dc.titleTwo models of murine B lymphopoiesis: A correlationen_US
dc.typeArticleen_US
dc.identifier.emailLu, L:liweilu@hkucc.hku.hken_US
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1002/(SICI)1521-4141(199806)28:06<1755::AID-IMMU1755>3.0.CO;2-3en_US
dc.identifier.pmid9645356-
dc.identifier.scopuseid_2-s2.0-0031841659en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031841659&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume28en_US
dc.identifier.issue6en_US
dc.identifier.spage1755en_US
dc.identifier.epage1761en_US
dc.identifier.isiWOS:000074258400003-
dc.publisher.placeGermanyen_US

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