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Article: Apoptosis during B lymphopoiesis in mouse bone marrow

TitleApoptosis during B lymphopoiesis in mouse bone marrow
Authors
Issue Date1997
PublisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.org
Citation
Journal Of Immunology, 1997, v. 158 n. 11, p. 5136-5145 How to Cite?
AbstractTo evaluate the magnitude of cell death and the critical stages at which it occurs during B lymphopoiesis in mouse bone marrow (BM), we have examined the kinetics of apoptosis at defined stages of B cell differentiation. FACS-sorted B220+ BM cells exhibited a low incidence of morphologically apoptotic cells by electron microscopy. In freshly prepared BM suspensions, the incidence of hypodiploid cells detected by multiparameter flow cytometry was greater among large dividing B220+ surface lgM- (slgM-) precursor B cells and slgMlow immature B lymphocytes than among terminal deoxynucleotidyl transferase+ (TdT+) pro-B cells, small nondividing B220+slgM- precursors, and surface lgD+ mature B lymphocytes. During short-term culture, apoptotic cells, identified by both DNA content and in situ DNA strand break labeling, increased linearly with time without macrophage ingestion, providing an assay for the rate of entry into apoptosis. B220+ B lineage cells accumulated in apoptosis more rapidly than cells of other lineages. The apoptotic rate was greater among B220+slgM- precursor cells than slgM+ B cells, and was highest among B220+μ- pro-B cells. Coculture with stromal cells reduced the apoptotic rate of B220+slgM- precursors to a greater extent than that of slgM+ B lymphocytes. The results lead to estimates of the actual number of B lineage cells undergoing apoptosis per unit time in successive differentiation compartments. The findings indicate that, although influenced by local microenvironmental factors, apoptotic cell death occurs most markedly at two developmental stages associated with lg heavy chain gene rearrangement and Ag receptor expression, respectively.
Persistent Identifierhttp://hdl.handle.net/10722/148094
ISSN
2015 Impact Factor: 4.985
2015 SCImago Journal Rankings: 3.549
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLu, Len_US
dc.contributor.authorOsmond, DGen_US
dc.date.accessioned2012-05-29T06:10:48Z-
dc.date.available2012-05-29T06:10:48Z-
dc.date.issued1997en_US
dc.identifier.citationJournal Of Immunology, 1997, v. 158 n. 11, p. 5136-5145en_US
dc.identifier.issn0022-1767en_US
dc.identifier.urihttp://hdl.handle.net/10722/148094-
dc.description.abstractTo evaluate the magnitude of cell death and the critical stages at which it occurs during B lymphopoiesis in mouse bone marrow (BM), we have examined the kinetics of apoptosis at defined stages of B cell differentiation. FACS-sorted B220+ BM cells exhibited a low incidence of morphologically apoptotic cells by electron microscopy. In freshly prepared BM suspensions, the incidence of hypodiploid cells detected by multiparameter flow cytometry was greater among large dividing B220+ surface lgM- (slgM-) precursor B cells and slgMlow immature B lymphocytes than among terminal deoxynucleotidyl transferase+ (TdT+) pro-B cells, small nondividing B220+slgM- precursors, and surface lgD+ mature B lymphocytes. During short-term culture, apoptotic cells, identified by both DNA content and in situ DNA strand break labeling, increased linearly with time without macrophage ingestion, providing an assay for the rate of entry into apoptosis. B220+ B lineage cells accumulated in apoptosis more rapidly than cells of other lineages. The apoptotic rate was greater among B220+slgM- precursor cells than slgM+ B cells, and was highest among B220+μ- pro-B cells. Coculture with stromal cells reduced the apoptotic rate of B220+slgM- precursors to a greater extent than that of slgM+ B lymphocytes. The results lead to estimates of the actual number of B lineage cells undergoing apoptosis per unit time in successive differentiation compartments. The findings indicate that, although influenced by local microenvironmental factors, apoptotic cell death occurs most markedly at two developmental stages associated with lg heavy chain gene rearrangement and Ag receptor expression, respectively.en_US
dc.languageengen_US
dc.publisherAmerican Association of Immunologists. The Journal's web site is located at http://www.jimmunol.orgen_US
dc.relation.ispartofJournal of Immunologyen_US
dc.subject.meshAnimalsen_US
dc.subject.meshApoptosisen_US
dc.subject.meshB-Lymphocytes - Immunology - Pathologyen_US
dc.subject.meshBone Marrow - Immunology - Pathologyen_US
dc.subject.meshCell Differentiationen_US
dc.subject.meshCells, Cultureden_US
dc.subject.meshCoculture Techniquesen_US
dc.subject.meshMaleen_US
dc.subject.meshMiceen_US
dc.subject.meshMice, Inbred C3hen_US
dc.subject.meshStromal Cells - Pathologyen_US
dc.titleApoptosis during B lymphopoiesis in mouse bone marrowen_US
dc.typeArticleen_US
dc.identifier.emailLu, L:liweilu@hkucc.hku.hken_US
dc.identifier.authorityLu, L=rp00477en_US
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.pmid9164929-
dc.identifier.scopuseid_2-s2.0-0031156933en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0031156933&selection=ref&src=s&origin=recordpageen_US
dc.identifier.volume158en_US
dc.identifier.issue11en_US
dc.identifier.spage5136en_US
dc.identifier.epage5145en_US
dc.identifier.isiWOS:A1997XA06300013-
dc.publisher.placeUnited Statesen_US

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