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Article: Monocyte chemoattractant protein-1 expression and macrophage infiltration in gliomas

TitleMonocyte chemoattractant protein-1 expression and macrophage infiltration in gliomas
Authors
KeywordsGlioma
Macrophage
Microglia
Monocyte chemoattractant protein-1
Issue Date1997
PublisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00401/index.htm
Citation
Acta Neuropathologica, 1997, v. 93 n. 5, p. 518-527 How to Cite?
AbstractWhile the number of reports on macrophage infiltration of gliomas is increasing, the extent and mechanisms of macrophage recruitment remain unclear. To investigate whether monocyte chemoattractant protein-1 (MCP-1) plays a role in this process, in situ hybridisation (ISH) was performed for 22 glioblastomas (GBM), 1 anaplastic astrocytoma (AA) and 4 grade II fibrillary astrocytomas (AII) and reverse transcription-polymerase chain reaction was performed in 13 GBM, 1 AA and 3 AII. High levels of MCP-1 mRNA were detectable in most GBM, while a lower level was detected in AII. Many tumour-associated macrophages (TAM) could be demonstrated by immunohistochemistry (IHC) in most GBM, while the AII contained a lower number of TAM. The positive correlation between the MCP-1 level and abundance of TAM suggested that MCP-1 has a role in TAM recruitment. By combining ISH and IHC, high levels of MCP-1 mRNA were shown both in tumour cells and TAM. Along tumour borders, reactive astrocytes and microglia also expressed MCP-1. In areas with T lymphocyte infiltration, larger numbers of MCP-1-positive cells with an enhanced level of expression could be identified. We propose that the mechanism of macrophage recruitment is, at least partly, effected by constitutive expression and T cell-mediated up-regulation of MCP-1 in tumour cells and TAM. The production of MCP-1 by TAM establishes a positive amplification circuit for macrophage recruitment in gliomas.
Persistent Identifierhttp://hdl.handle.net/10722/148087
ISSN
2015 Impact Factor: 11.36
2015 SCImago Journal Rankings: 6.610
ISI Accession Number ID
References

 

DC FieldValueLanguage
dc.contributor.authorLeung, SYen_HK
dc.contributor.authorWong, MPen_HK
dc.contributor.authorChung, LPen_HK
dc.contributor.authorChan, ASYen_HK
dc.contributor.authorYuen, STen_HK
dc.date.accessioned2012-05-29T06:10:46Z-
dc.date.available2012-05-29T06:10:46Z-
dc.date.issued1997en_HK
dc.identifier.citationActa Neuropathologica, 1997, v. 93 n. 5, p. 518-527en_HK
dc.identifier.issn0001-6322en_HK
dc.identifier.urihttp://hdl.handle.net/10722/148087-
dc.description.abstractWhile the number of reports on macrophage infiltration of gliomas is increasing, the extent and mechanisms of macrophage recruitment remain unclear. To investigate whether monocyte chemoattractant protein-1 (MCP-1) plays a role in this process, in situ hybridisation (ISH) was performed for 22 glioblastomas (GBM), 1 anaplastic astrocytoma (AA) and 4 grade II fibrillary astrocytomas (AII) and reverse transcription-polymerase chain reaction was performed in 13 GBM, 1 AA and 3 AII. High levels of MCP-1 mRNA were detectable in most GBM, while a lower level was detected in AII. Many tumour-associated macrophages (TAM) could be demonstrated by immunohistochemistry (IHC) in most GBM, while the AII contained a lower number of TAM. The positive correlation between the MCP-1 level and abundance of TAM suggested that MCP-1 has a role in TAM recruitment. By combining ISH and IHC, high levels of MCP-1 mRNA were shown both in tumour cells and TAM. Along tumour borders, reactive astrocytes and microglia also expressed MCP-1. In areas with T lymphocyte infiltration, larger numbers of MCP-1-positive cells with an enhanced level of expression could be identified. We propose that the mechanism of macrophage recruitment is, at least partly, effected by constitutive expression and T cell-mediated up-regulation of MCP-1 in tumour cells and TAM. The production of MCP-1 by TAM establishes a positive amplification circuit for macrophage recruitment in gliomas.en_HK
dc.languageengen_US
dc.publisherSpringer Verlag. The Journal's web site is located at http://link.springer.de/link/service/journals/00401/index.htmen_HK
dc.relation.ispartofActa Neuropathologicaen_HK
dc.subjectGliomaen_HK
dc.subjectMacrophageen_HK
dc.subjectMicrogliaen_HK
dc.subjectMonocyte chemoattractant protein-1en_HK
dc.subject.meshAstrocytoma - Metabolism - Pathologyen_US
dc.subject.meshBrain Neoplasms - Metabolism - Pathologyen_US
dc.subject.meshChemokine Ccl2 - Biosynthesisen_US
dc.subject.meshGlioblastoma - Metabolism - Pathologyen_US
dc.subject.meshGlioma - Metabolism - Pathologyen_US
dc.subject.meshHumansen_US
dc.subject.meshImmunohistochemistryen_US
dc.subject.meshIn Situ Hybridizationen_US
dc.subject.meshMacrophages - Physiologyen_US
dc.subject.meshPolymerase Chain Reactionen_US
dc.subject.meshRna, Messenger - Biosynthesisen_US
dc.subject.meshT-Lymphocytes - Physiologyen_US
dc.titleMonocyte chemoattractant protein-1 expression and macrophage infiltration in gliomasen_HK
dc.typeArticleen_HK
dc.identifier.emailLeung, SY: suetyi@hku.hken_HK
dc.identifier.emailWong, MP: mwpik@hkucc.hku.hken_HK
dc.identifier.emailChung, LP: lpchung@hkucc.hku.hken_HK
dc.identifier.authorityLeung, SY=rp00359en_HK
dc.identifier.authorityWong, MP=rp00348en_HK
dc.identifier.authorityChung, LP=rp00249en_HK
dc.description.naturelink_to_subscribed_fulltexten_US
dc.identifier.doi10.1007/s004010050647en_HK
dc.identifier.pmid9144591-
dc.identifier.scopuseid_2-s2.0-0030951515en_HK
dc.identifier.hkuros26134en_US
dc.relation.referenceshttp://www.scopus.com/mlt/select.url?eid=2-s2.0-0030951515&selection=ref&src=s&origin=recordpageen_HK
dc.identifier.volume93en_HK
dc.identifier.issue5en_HK
dc.identifier.spage518en_HK
dc.identifier.epage527en_HK
dc.identifier.isiWOS:A1997WW29400012-
dc.publisher.placeGermanyen_HK
dc.identifier.scopusauthoridLeung, SY=7202044886en_HK
dc.identifier.scopusauthoridWong, MP=7403907887en_HK
dc.identifier.scopusauthoridChung, LP=24315879100en_HK
dc.identifier.scopusauthoridChan, ASY=55246984000en_HK
dc.identifier.scopusauthoridYuen, ST=7103160927en_HK

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